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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> tRNAs
Overview
Product name
Anti-EEF1A2 antibody
See all EEF1A2 products (3) ...
Description
Rabbit polyclonal to EEF1A2
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Cow, Pig, Zebrafish
Immunogen
Synthetic peptide: VIDCHTAHIA CKFAELKEKI DRRSGKKLED NPKSLKSGDA AIVEMVPGKP, corresponding to a region within internal sequence amino acids 360 - 409 of Human EEF1A2 (NP_001949)
VIDCHTAHIA CKFAELKEKI DRRSGKKLED NPKSLKSGDA AIVEMVPGKP
Positive control
Fetal brain lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Transcription factors
Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> tRNAs
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Western blot - EEF1A2 antibody (ab82912)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab82912 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
ELISA
Application notes
ELISA: Use at an assay dependent dilution.
ELISA titre using peptide based assay: 1/12500
WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 50 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.
Tissue specificity
Brain, heart, and skeletal muscle.
Sequence similarities
Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ05639
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- eEF1A-2 antibody
- Eef1a2 antibody
- EEF1AL antibody
see all
Database links
- Entrez Gene: 515233 Cow
- Entrez Gene: 1917 Human
- Entrez Gene: 13628 Mouse
- Entrez Gene: 24799 Rat
- Omim: 602959 Human
- SwissProt: Q32PH8 Cow
- SwissProt: Q05639 Human
- SwissProt: P62631 Mouse
see all
Anti-EEF1A2 antibody images:
Western blot - EEF1A2 antibody (ab82912)
Anti-EEF1A2 antibody (ab82912) at 1 µg/ml (in 5% skim milk / PBS buffer) + fetal brain lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 50 kDa
Observed band size : 50 kDa
Gel concentration 12%
References for Anti-EEF1A2 antibody (ab82912)
ab82912 has not yet been referenced specifically in any publications.
Publishing research using ab82912? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"