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Western blot - EEF1A2 antibody (ab82912)
(enlarge)
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Product Name
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EEF1A2 antibody
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to EEF1A2
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Immunogen
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Synthetic peptide: VIDCHTAHIA CKFAELKEKI DRRSGKKLED NPKSLKSGDA AIVEMVPGKP, corresponding to a region within internal sequence amino acids 360 - 409 of Human EEF1A2 (NP_001949)
BLAST 'VIDCHTAHIA CKFAELKEKI DRRSGKKLED NPKSLKSGDA AIVEMVPGKP ' with  or 
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Reacts with
(species key)
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Hu, Ms, Rat
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Tested applications
(see key)
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ELISA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: Use at an assay dependent dilution.
ELISA titre using peptide based assay: 1/12500
WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 50 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Fetal brain lysate
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Cellular localization
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Nuclear
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Research areas
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Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Transcription factors
Chromatin and Nuclear Signaling >> DNA / RNA >> Translation >> tRNAs
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Relevance
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EEF1A2 is responsible for the GTP dependent enzymatic delivery of aminoacyl tRNAs to the A-site of ribosomes during protein biosynthesis. This isoform (alpha 2) is expressed in brain, heart and skeletal muscle, and the other isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas. This gene may be critical in the development of ovarian cancer.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: None
Constituents: 2% Sucrose, PBS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this EEF1A2 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for EEF1A2 antibody images, references, products related to ab82912 and other tools.
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EEF1A2 antibody images:
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 Western blot - EEF1A2 antibody (ab82912)
EEF1A2 antibody (ab82912) at 1 µg/ml (in 5% skim milk / PBS buffer) + fetal brain lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 50 kDa
Observed band size : 50 kDa
Gel concentration 12%
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Search PubMed (MEDLINE) for references to EEF1A2
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EEF1A2 antibody - more information
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Customer reviews (feedback) regarding EEF1A2 antibody |
Customer FAQs regarding EEF1A2 antibody |
Protocols for EEF1A2 antibody |
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EEF1A2 antibody - related products:
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EEF1A2 antibody - other tools:
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Contact Abcam with a Technical Enquiry about EEF1A2 antibody |
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