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Anti-EEF2 antibody [EP723Y] (ab40762)

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Overview

Product name

Anti-EEF2 antibody [EP723Y]
See all EEF2 products (13) ...

Description

Rabbit monoclonal [EP723Y] to EEF2

Tested applications

WB, IHC-P, ICC/IFmore details

Cross reactivity

Reacts with

Mouse, Rat, Human

Immunogen

A synthetic peptide corresponding to residues near the N-terminus of human eEF2 was used as immunogen.

Positive control

293 cell lysate, human lymphoma (IHC-P).

General notes

Produced under U.S. Patent No. 5,675,063.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%

Purity

Tissue culture supernatant

Clonality

Monoclonal

Clone number

EP723Y

Isotype

IgG

Research areas

Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> Regulation

  • Western blot - EEF2 antibody [EP723Y] (ab40762)Western blot - EEF2 antibody [EP723Y] (ab40762) image (enlarge)

  • Immunohistochemistry (Paraffin-embedded sections) - EEF2 antibody [EP723Y] (ab40762)Immunohistochemistry (Paraffin-embedded sections) - EEF2 antibody [EP723Y] (ab40762) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab40762 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

ICC/IF: 1/50.
IHC-P: 1/50 - 1/100.

WB: 1/1000. Detects a band of approximately 95 kDa (predicted molecular weight: 95 kDa).

Is unsuitable for FACS or IP.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.

Sequence similarities

Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily.

Post-translational
modifications

Phosphorylation by EF-2 kinase completely inactivates EF-2.
Diphthamide is 2-[3-carboxyamido-3-(trimethyl-ammonio)propyl]histidine. Diphthamide can be ADP-ribosylated by diphtheria toxin and by Pseudomonas exotoxin A, thus arresting protein synthesis.
ISGylated.

Cellular localization

Cytoplasm.

Target information above from: UniProt accessionP13639 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • EEF 2 antibody
  • Eef2 antibody
  • EF-2 antibody
  • EF2 antibody
  • EF2_HUMAN antibody
  • Elongation factor 2 antibody
  • Eukaryotic translation elongation factor 2 antibody
  • Polypeptidyl tRNA translocase antibody
see all

Database links

see all

Anti-EEF2 antibody [EP723Y] images:

  Western blot - EEF2 antibody [EP723Y] (ab40762)

Western blot - EEF2 antibody [EP723Y] (ab40762)

Anti-EEF2 antibody [EP723Y] (ab40762) at 1/1000 dilution + HEK293 cell lysate at 10 µg

Predicted band size : 95 kDa
Observed band size : 95 kDa

  Immunohistochemistry (Paraffin-embedded sections) - EEF2 antibody [EP723Y] (ab40762)

Immunohistochemistry (Paraffin-embedded sections) - EEF2 antibody [EP723Y] (ab40762)

ab40762 (1/50) staining human lymphoma by immunohistochemistry, paraffin embedded tissue.

References for Anti-EEF2 antibody [EP723Y] (ab40762)

ab40762 has not yet been referenced specifically in any publications.

Publishing research using ab40762? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"