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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »This product is covered by the Abpromise guarantee. Our scientific support team are available to answer any questions or queries - fill out an inquiry form for ab75748 for help.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
![Western blot - EEF2 antibody [EP880Y] - Carboxyterminal end (ab75748)](/ps/datasheet/Images/75/ab75748/ab75748_1.jpg)
Western blot - EEF2 antibody [EP880Y] - Carboxyterminal end (ab75748)
All lanes : Anti-EEF2 antibody [EP880Y] - C-terminal (ab75748) at 1/10000 dilution
Lane 1 : 293 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : A431 cell lysate
Lane 4 : NIH3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size : 95 kDa
Observed band size : 95 kDa
Additional bands at : 30 kDa,70 kDa. We are unsure as to the identity of these extra bands.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - EEF2 antibody [EP880Y] - C-terminal (ab75748)](/ps/datasheet/Images/75/ab75748/ab75748_4.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - EEF2 antibody [EP880Y] - C-terminal (ab75748)
ab75748 at 1/100 dilution staining EEF2 in human kidney carcinoma tissue.
![Immunocytochemistry/ Immunofluorescence-EEF2 antibody [EP880Y] - C-terminal(ab75748)](/ps/datasheet/images/75/ab75748/EEF2-Primary-antibodies-ab75748-1.jpg)
Immunocytochemistry/ Immunofluorescence-EEF2 antibody [EP880Y] - C-terminal(ab75748)
ICC/IF image of ab75748 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab75748, 1µg/ml) overnight at +4ºC. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
![Flow Cytometry - Anti-EEF2 antibody [EP880Y] - C-terminal (ab75748)](/ps/datasheet/images/75/ab75748/EEF2-Primary-antibodies-ab75748-2.jpg)
Flow Cytometry - Anti-EEF2 antibody [EP880Y] - C-terminal (ab75748)
Overlay histogram showing HeLa cells stained with ab75748 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab75748, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (
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