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Anti-EEF2 antibody [EP880Y] - C-terminal (ab75748)

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Overview

Product name

Anti-EEF2 antibody [EP880Y] - C-terminal
See all EEF2 products (11) ...

Description

Rabbit monoclonal [EP880Y] to EEF2 - C-terminal

Tested applications

WB, IP, Flow Cyt, IHC-P, ICC, ICC/IFmore details

Cross reactivity

Reacts with

Mouse, Rat, Human

Immunogen

A synthetic peptide corresponding to residues on the C terminal of human EEF2.

Positive control

A431, NIH3T3, HeLa or 293 cell lysates. Human kidney carcinoma tissue.

General notes

Produced under U.S. Patent No. 5,675,063.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, Tissue culture supernatant, 0.15M Sodium chloride, 50mM Tris glycine, pH 7.4

Purity

Tissue culture supernatant

Clonality

Monoclonal

Clone number

EP880Y

Isotype

IgG

Research areas

Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> Regulation

  • Western blot - EEF2 antibody [EP880Y] - Carboxyterminal end (ab75748)Western blot - EEF2 antibody [EP880Y] - Carboxyterminal end (ab75748) image (enlarge)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - EEF2 antibody [EP880Y] - C-terminal (ab75748)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - EEF2 antibody [EP880Y] - C-terminal (ab75748) image (enlarge)

  • Immunocytochemistry/ Immunofluorescence-EEF2 antibody [EP880Y] - C-terminal(ab75748)Immunocytochemistry/ Immunofluorescence-EEF2 antibody [EP880Y] - C-terminal(ab75748) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab75748 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide1 Image

    WB

     WB: 1/10000 - 1/20000.De...Read more →

    WB: 1/10000 - 1/20000.Detects a band of approximately 95 kDa (predicted molecular weight: 95 kDa).

  • IP

     IP: 1/20

    IP: 1/20

  • 1 Image

    Flow Cyt

     Flow Cyt: 1/20

    Flow Cyt: 1/20

  • ShowHide1 Image

    IHC-P

     IHC-P: 1/100 - 1/250.(Pe...Read more →

    IHC-P: 1/100 - 1/250.(Perform heat mediated antigen retrieval using 0.01M Sodium Citrate Buffer, pH 6.0 before commencing with IHC staining protocol.)

  • ICC

     ICC: 1/100 - 1/250.

    ICC: 1/100 - 1/250.

  • ShowHide1 Image

    ICC/IF

     ICC/IF: Use a concentrat...Read more →

    ICC/IF: Use a concentration of 1 µg/ml

Target

Function

Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.

Sequence similarities

Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily.

Post-translational
modifications

Phosphorylation by EF-2 kinase completely inactivates EF-2.
Diphthamide is 2-[3-carboxyamido-3-(trimethyl-ammonio)propyl]histidine. Diphthamide can be ADP-ribosylated by diphtheria toxin and by Pseudomonas exotoxin A, thus arresting protein synthesis.
ISGylated.

Cellular localization

Cytoplasm.

Target information above from: UniProt accessionP13639 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • EEF 2 antibody
  • Eef2 antibody
  • EF-2 antibody
  • EF2 antibody
  • EF2_HUMAN antibody
  • Elongation factor 2 antibody
  • Eukaryotic translation elongation factor 2 antibody
  • Polypeptidyl tRNA translocase antibody
see all

Database links

see all

Anti-EEF2 antibody [EP880Y] - C-terminal images:

  Western blot - EEF2 antibody [EP880Y] - Carboxyterminal end (ab75748)

Western blot - EEF2 antibody [EP880Y] - Carboxyterminal end (ab75748)

All lanes : Anti-EEF2 antibody [EP880Y] - C-terminal (ab75748) at 1/10000 dilution

Lane 1 : 293 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : A431 cell lysate
Lane 4 : NIH3T3 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size : 95 kDa
Observed band size : 95 kDa
Additional bands at : 30 kDa,70 kDa. We are unsure as to the identity of these extra bands.

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - EEF2 antibody [EP880Y] - C-terminal (ab75748)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - EEF2 antibody [EP880Y] - C-terminal (ab75748)

ab75748 at 1/100 dilution staining EEF2 in human kidney carcinoma tissue.

  Immunocytochemistry/ Immunofluorescence-EEF2 antibody [EP880Y] - C-terminal(ab75748)

Immunocytochemistry/ Immunofluorescence-EEF2 antibody [EP880Y] - C-terminal(ab75748)

ICC/IF image of ab75748 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab75748, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  Flow Cytometry - Anti-EEF2 antibody [EP880Y] - C-terminal (ab75748)

Flow Cytometry - Anti-EEF2 antibody [EP880Y] - C-terminal (ab75748)

Overlay histogram showing HeLa cells stained with ab75748 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab75748, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References for Anti-EEF2 antibody [EP880Y] - C-terminal (ab75748)

ab75748 has not yet been referenced specifically in any publications.

Publishing research using ab75748? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"