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Western blot - EEF2 antibody [EP880Y] - Carboxyterminal end (ab75748)
![Western blot - EEF2 antibody [EP880Y] - Carboxyterminal end (ab75748)](/ps/datasheet/images/ab75748_1.jpg)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - EEF2 antibody [EP880Y] - C-terminal (ab75748)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - EEF2 antibody [EP880Y] - C-terminal (ab75748)](/ps/datasheet/images/ab75748_4.jpg)
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Product Name
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EEF2 antibody [EP880Y] - C-terminal
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See all EEF2 antibodies (10)...
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Product type
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Primary antibodies
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Description
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Rabbit monoclonal [EP880Y] to EEF2 - C-terminal
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Immunogen
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A synthetic peptide corresponding to residues on the C terminal of human EEF2.
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Reacts with
(species key)
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Hu, Ms, Rat
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Tested applications
(see key)
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Flow Cyt, ICC, IHC-P, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
Flow Cyt: 1/20.
ICC: 1/100 - 1/250.
IHC-P: 1/100 - 1/250. Perform heat mediated antigen retrieval using 0.01M Sodium Citrate Buffer, pH 6.0 before commencing with IHC staining protocol.
IP: 1/20.
WB: 1/10000 - 1/20000. Detects a band of approximately 95 kDa (predicted molecular weight: 95 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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A431, NIH3T3, HeLa or 293 cell lysates. Human kidney carcinoma tissue.
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Cellular localization
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Cytoplasmic
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Research areas
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Chromatin and Nuclear Signaling >> DNA / RNA >> Translation >> Regulation
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Relevance
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EEF2 is a member of the GTP-binding translation elongation factor family. This protein is an essential factor for protein synthesis. It promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. This protein is completely inactivated by EF-2 kinase phosporylation.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Monoclonal
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Clone number
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EP880Y
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Isotype
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IgG
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Purity
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Tissue culture supernatant
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Storage buffer
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Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, Tissue culture supernatant, 0.15M Sodium chloride, 50mM Tris glycine, pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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Concentration not determined - why is this?
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Notes
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Produced under U.S. Patent No. 5,675,063.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this EEF2 antibody [EP880Y] - C-terminal is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for EEF2 antibody [EP880Y] - C-terminal images, references, products related to ab75748 and other tools.
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EEF2 antibody [EP880Y] - C-terminal images:
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 Western blot - EEF2 antibody [EP880Y] - Carboxyterminal end (ab75748)
All lanes : EEF2 antibody [EP880Y] - C-terminal (ab75748) at 1/10000 dilution
Lane 1 : 293 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : A431 cell lysate
Lane 4 : NIH3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size : 95 kDa
Observed band size : 95 kDa
Additional bands at : 30 kDa,70 kDa. We are unsure as to the identity of these extra bands.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - EEF2 antibody [EP880Y] - C-terminal (ab75748)
ab75748 at 1/100 dilution staining EEF2 in human kidney carcinoma tissue.
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Search PubMed (MEDLINE) for references to EEF2
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EEF2 antibody [EP880Y] - C-terminal - more information
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Customer reviews (feedback) regarding EEF2 antibody [EP880Y] - C-terminal |
Customer FAQs regarding EEF2 antibody [EP880Y] - C-terminal |
Protocols for EEF2 antibody [EP880Y] - C-terminal |
Price and availability of products related to EEF2 antibody [EP880Y] - C-terminal |
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EEF2 antibody [EP880Y] - C-terminal - related products:
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EEF2 antibody [EP880Y] - C-terminal - other tools:
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Contact Abcam with a Technical Enquiry about EEF2 antibody [EP880Y] - C-terminal |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
![Western blot - EEF2 antibody [EP880Y] - Carboxyterminal end (ab75748)](/index.html?datasheet=75748#ab75748_1.jpg)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - EEF2 antibody [EP880Y] - C-terminal (ab75748)](/index.html?datasheet=75748#ab75748_4.jpg)
