You have changed your country from to
. Please be aware that this will change the currency in the purchasing process.
- Primary antibodies
- Secondary antibodies
-
Proteins & Peptides
-
A-Z
- Full length proteins
- Partial proteins
- Peptides
-
Enzymes
- MMPs
- Caspases
- Kinases and transferases
- Signal transduction related
-
Cytokines and growth factors
- Cytokines
- Growth factors by family
-
Human proteins
- Human expressed
- Barley expressed
- Wheat expressed
- Fc fusion proteins
- Ig proteins
-
His-tagged Proteins by research area
- Cancer
- Cardiovascular
- Epigenetics & Nuclear Signaling
- Cell Biology
- Immunology
- Neuroscience
- Signal Transduction
- Stem Cells
-
Microbiology and virology
- All microbiology and viral proteins
- DNA virus proteins
- RNA viral proteins
- All Hepatitis virus proteins
- HIV proteins
- HSV, Measles and Influenza proteins
- Bacterial toxins and chaperones
-
Resources
- Advantages of expression in barley
-
-
ELISA Kits
-
A-Z
- ELISA Kits
- ELIPAIR Kits
- ELISPOT Kits
- FLUOROSPOT Kits
-
ELISA development
- Matched antibody pairs
-
Resources
- Immunoassay technical know-how
- ELISA and ELIPAIR
- ELISPOT and FLUOROSPOT
-
ELISA Kits by protein family
- Adaptive immune components
- Adhesion molecules
- Angiogenic factors
- Apoptosis markers
- Atherosclerotic proteins
- Blood cell antigens
- Cancer metabolism proteins
- Cardiovascular proteins
- CD markers
- Cell cycle proteins
- Complement activation
- Cytokines and cytokine receptors
- Developmental proteins
- Granzymes and perforins
- Growth factors/hormones
- Immunoglobulins
- Oncoproteins
- Pathogen specific immunoglobulins
- Protease inhibitors
- Signal transduction proteins
- Sub cellular markers
- Tumor biomarkers
-
-
Assay Kits
-
A-Z
- Apoptosis kits
- Epigenetic kits
- Metabolism kits
- ChIP kits
-
Metabolism kits by analyte
- Alcohol
- Amino acids
- Calcium
- Cell viability and senescence
- Coenzymes and cofactors
- Creatinine
- Inorganic ions
- Intermediary metabolism
- Lipid metabolism
- Nitric oxide
- Phosphates
- Sugars
- Oxidative stress
- Other metabolism assays
-
Apoptosis kits by analyte
- Annexin V
- Calpain
- Caspases
- Cathepsins
- DNA fragmentation
- Transmembrane potential
- Other Apoptosis assays
-
Epigenetic Kits by modification
- DNA modification and methylation
- Histone demethylation
- Histone methylation activity
- Sample Preparation
- Histone methylation quantification
- Histone acetylation and deacetylation
- Histone phosphorylation
- Sumoylation
-
-
Other Kits
-
A-Z
- All kits
-
Antibody labeling
- EasyLink antibody conjugation kits
-
Cell fractionation
- Cell fractionation kits A-Z
-
Cell Staining Kits
- CytoPainter kits
-
-
Biochemicals
A-Z
- By pharmacology
- By research area
- By chemical type
- By product range
Receptors & Transporters
- Adenosine
- Adrenoceptors
- Cannabinoid
- Catalytic Receptors
- Dopamine
- GABA
- Glutamate
- Glycine
- Histamine
- Imidazoline
- Melatonin
- Muscarinic
- Nicotinic
- Nuclear Receptors
- Opioids
- Peptide Receptors
- Purinergics
- Serotonin
- Transporters
-
Immunodetection
-
IHC Kits
- EXPOSE biotin-free detection kits
- Biotin-Streptavidin detection kits
- Endogenous avidin/biotin blocking systems
- Substrate kits
- Mouse on Mouse detection kit
- BrdU IHC detection kit
-
Secondary antibodies
- Advanced search
-
Reagents and buffers
- Buffers
- Chromogens and enhancers
- Diluents
- Epitope recovery and pretreatments
- Fluorescent ligands
- Mounting media
- PAP pen
- Serum
- Substrates
-
-
Controls
-
A-Z
- Isotype controls
- Loading controls
- Lysates
-
Isotype controls by host species
- Chicken
- Goat
- Hamster (Armenian and Syrian)
- Mouse
- Rabbit
- Rat
- Sheep
-
Lysates by type
- 293T transfected
- Cytoplasmic
- Membrane
- Nuclear
- Tissue
- Total protein
- Whole cell
-
Loading controls
- Loading controls by protein target
-
Product partner
Related products
-
Proteins and Peptides
ab33521
-
Secondary antibodies
Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab33208? Please let us know so that we can cite the reference in this datasheet
ab33208 has been referenced in 1 publications.
- Mockett BG et al. Calcium/Calmodulin-Dependent Protein Kinase II Mediates Group I Metabotropic Glutamate Receptor-Dependent Protein Synthesis and Long-Term Depression in Rat Hippocampus. J Neurosci 31:7380-91 (2011). WB; Rat. PubMed: 21593322
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - EEF2 antibody (ab33208)
All lanes : Anti-EEF2 antibody (ab33208) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 :
Lane 3 :
Lane 4 :
Lane 5 :
Lane 6 : MCF-7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 7 : SHSY-5Y (Human Neuroblastoma) Whole Cell Lysate
Lane 8 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size : 95 kDa
Observed band size : 95 kDa
Immunocytochemistry/ Immunofluorescence - EEF2 antibody (ab33208)
ICC/IF image of ab33208 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab33208, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
0
Events
Talk to us
Special offers
© 1998-2012 Abcam plc. All rights reserved.
United States
0
- A - C
- Albania
- Algeria
- Antigua and Barbuda
- Argentina
- Armenia
- Aruba
- Australia
- Austria
- Azerbaijan
- Bahamas
- Bahrain
- Bangladesh
- Barbados
- Belarus
- Belgium
- Belize
- Bermuda
- Bolivia
- Bosnia and herzegovina
- Botswana
- Brazil
- Brunei darussalam
- Bulgaria
- Cambodia
- Cameroon
- Canada
- Cayman islands
- Chile
- China
- Colombia
- Costa rica
- Cote d'ivoire
- Croatia
- Cyprus
- Czech Republic
- D - I
- Denmark
- Dominica
- Dominican republic
- Ecuador
- Egypt
- El salvador
- Estonia
- Ethiopia
- Finland
- France
- French Guiana
- Gabon
- Gambia
- Georgia
- Germany
- Ghana
- Greece
- Grenada
- Guadeloupe
- Guam
- Guatemala
- Guyana
- Haiti
- Honduras
- Hong Kong
- Hungary
- Iceland
- India
- Indonesia
- Ireland
- Israel
- Italy
- J - O
- Jamaica
- Japan
- Jordan
- Kazakhstan
- Kenya
- Korea, Rep of
- Kuwait
- Kyrgystan
- Latvia
- Lebanon
- Lithuania
- Luxembourg
- Macau
- Macedonia
- Madagascar
- Malawi
- Malaysia
- Malta
- Martinique
- Mexico
- Monaco
- Morocco
- Nepal
- Netherlands
- Netherlands Ant
- New caledonia
- New Zealand
- Nicaragua
- Nigeria
- Norway
- Oman
Call 01223 696 000 or contact us