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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> Regulation
Overview
Product name
Anti-EEF2 antibody
See all EEF2 products (11) ...
Description
Rabbit polyclonal to EEF2
Tested applications
ICC/IF, WBmore details
Cross reactivity
Reacts with
Rat, Human
Predicted to work with
Mouse, Chicken, Hamster, Cow, Xenopus laevis, Zebrafish
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 550 - 650 of Human EEF2.
(Peptide available as ab33521.)
Positive control
This antibody gave a positive signal in the following Whole Cell Lysates: HeLa; Jurkat; A431; HEK 293; HepG2; MCF-7; SHSY-5Y; U2OS.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> Regulation
Applications
Show applications key
Our Abpromise guarantee covers the use of ab33208 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use a concentration of 5 µg/ml
WB: Use a concentration of 1 µg/mlDetects a band of approximately 95 kDa (predicted molecular weight: 95 kDa).
Target
Function
Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.
Sequence similarities
Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily.
Post-translational
modifications
Phosphorylation by EF-2 kinase completely inactivates EF-2.
Diphthamide is 2-[3-carboxyamido-3-(trimethyl-ammonio)propyl]histidine. Diphthamide can be ADP-ribosylated by diphtheria toxin and by Pseudomonas exotoxin A, thus arresting protein synthesis.
ISGylated.
Cellular localization
Cytoplasm.
Target information above from: UniProt accessionP13639
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- EEF 2 antibody
- Eef2 antibody
- EF-2 antibody
see all
Database links
- Entrez Gene: 281138 Cow
- Entrez Gene: 1938 Human
- Entrez Gene: 13629 Mouse
- Entrez Gene: 29565 Rat
- Entrez Gene: 380348 Xenopus laevis
- Entrez Gene: 30373 Zebrafish
- Entrez Gene: 326929 Zebrafish
- Omim: 130610 Human
see all
Anti-EEF2 antibody images:
Western blot - EEF2 antibody (ab33208)
All lanes : Anti-EEF2 antibody (ab33208) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate (ab7899)
Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate (ab7909)
Lane 4 : HEK293 Human embryonic kidney cell line Whole Cell Lysate (ab7902)
Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate (ab7900)
Lane 6 : MCF-7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 7 : SHSY-5Y (Human Neuroblastoma) Whole Cell Lysate
Lane 8 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size : 95 kDa
Observed band size : 95 kDa
Immunocytochemistry/ Immunofluorescence - EEF2 antibody (ab33208)
ICC/IF image of ab33208 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab33208, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
References for Anti-EEF2 antibody (ab33208)
This product has been referenced in:
- Mockett BGet al. Calcium/Calmodulin-Dependent Protein Kinase II Mediates Group I Metabotropic Glutamate Receptor-Dependent Protein Synthesis and Long-Term Depression in Rat Hippocampus. J Neurosci 31:7380-91 (2011). WB; Rat.Read more (PubMed: 21593322) »
See 1 publication for this product
Publishing research using ab33208? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"