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Proteins and Peptides
ab33522
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Secondary antibodies
Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab33523? Please let us know so that we can cite the reference in this datasheet
ab33523 has been referenced in 1 publications.
- Abere B et al. Proteomic analysis of chikungunya virus infected microgial cells. PLoS One 7:e34800 (2012). WB. PubMed: 22514668
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - EEF2 antibody (ab33523)
All lanes : Anti-EEF2 antibody (ab33523) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 :
Lane 3 :
Lane 4 :
Lane 5 :
Lane 6 : MCF-7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 7 : SHSY-5Y (Human Neuroblastoma) Whole Cell Lysate
Lane 8 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size : 95 kDa
Observed band size : 95 kDa
Western blot - EEF2 antibody (ab33523)
All lanes : Anti-EEF2 antibody (ab33523) at 1 µg/ml
Lane 1 :
Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 3 :
Lane 4 : Ovary (Mouse) Tissue Lysate - normal tissue
Lane 5 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lane 6 : Liver (Rat) Tissue Lysate - normal tissue
Lysates/proteins at 10 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Predicted band size : 95 kDa
Observed band size : 95 kDa
Immunocytochemistry/ Immunofluorescence - EEF2 antibody (ab33523)
ICC/IF image of ab33523 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab33523, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
Western blot - Anti-EEF2 antibody (ab33523)
All lanes : Anti-EEF2 antibody (ab33523) at 1 µg/ml
Lane 1 : Marker
Lane 2 : Zebrafish brain homogenate (20ug)
Lane 3 : Zebrafish heart homogenate (20ug)
Lane 4 : Zebrafish liver homogenate (20ug)
Lane 5 : Zebrafish skeletal muscle homogenate (20ug)
Lane 6 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (20ug)
Secondary
Goat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) at 1/6000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 95 kDa
Observed band size : 95 kDa
Exposure time : 5 minutes
EEF2 antibody for WB in Human (33523)
EEF2 antibody for WB in Mouse (33523)
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