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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> Regulation
Overview
Product name
Anti-EEF2 antibody
See all EEF2 products (11) ...
Description
Rabbit polyclonal to EEF2
Tested applications
ICC/IF, WBmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Predicted to work with
Chicken, Hamster, Cow, Xenopus laevis, Zebrafish
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 400 - 500 of Human EEF2.
(Peptide available as ab33522.)
Positive control
This antibody gave a positive signal in the following whole cell lysates: HeLa; Jurkat; A431; HEK 293; HepG2; MCF-7; SHSY-5Y; U2OS; NIH 3T3; MEF1; PC12. This antibody gave a positive signal in the following tissue lysates: Mouse Pancreas; Mouse Ovary; Rat Liver.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> Regulation
Applications
Show applications key
Our Abpromise guarantee covers the use of ab33523 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use a concentration of 5 µg/ml
WB: Use at an assay dependent concentration. Detects a band of approximately 95 kDa (predicted molecular weight: 95 kDa).
Target
Function
Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.
Sequence similarities
Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily.
Post-translational
modifications
Phosphorylation by EF-2 kinase completely inactivates EF-2.
Diphthamide is 2-[3-carboxyamido-3-(trimethyl-ammonio)propyl]histidine. Diphthamide can be ADP-ribosylated by diphtheria toxin and by Pseudomonas exotoxin A, thus arresting protein synthesis.
ISGylated.
Cellular localization
Cytoplasm.
Target information above from: UniProt accessionP13639
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- EEF 2 antibody
- Eef2 antibody
- EF-2 antibody
see all
Database links
- Entrez Gene: 281138 Cow
- Entrez Gene: 1938 Human
- Entrez Gene: 13629 Mouse
- Entrez Gene: 29565 Rat
- Entrez Gene: 380348 Xenopus laevis
- Entrez Gene: 30373 Zebrafish
- Entrez Gene: 326929 Zebrafish
- Omim: 130610 Human
see all
Anti-EEF2 antibody images:
Western blot - EEF2 antibody (ab33523)
All lanes : Anti-EEF2 antibody (ab33523) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate (ab7899)
Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate (ab7909)
Lane 4 : HEK293 Human embryonic kidney cell line Whole Cell Lysate (ab7902)
Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate (ab7900)
Lane 6 : MCF-7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 7 : SHSY-5Y (Human Neuroblastoma) Whole Cell Lysate
Lane 8 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size : 95 kDa
Observed band size : 95 kDa
Western blot - EEF2 antibody (ab33523)
All lanes : Anti-EEF2 antibody (ab33523) at 1 µg/ml
Lane 1 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate (ab7179)
Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 3 : Pancreas (Mouse) Tissue Lysate (ab29363)
Lane 4 : Ovary (Mouse) Tissue Lysate - normal tissue
Lane 5 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lane 6 : Liver (Rat) Tissue Lysate - normal tissue
Lysates/proteins at 10 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Predicted band size : 95 kDa
Observed band size : 95 kDa
Immunocytochemistry/ Immunofluorescence - EEF2 antibody (ab33523)
ICC/IF image of ab33523 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab33523, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
References for Anti-EEF2 antibody (ab33523)
ab33523 has not yet been referenced specifically in any publications.
Publishing research using ab33523? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"