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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> Regulation
Overview
Product name
Anti-EEF2 antibody
See all EEF2 products (11) ...
Description
Rabbit polyclonal to EEF2
Tested applications
WB, ICC, Flow Cyt, IP, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide corresponding to residues in the C-terminus of Human EEF2.
Positive control
HEK293 and Human breast carcinoma tissue.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
pH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol, 0.87% Sodium chloride, 0.09% Tris glycine
Purity
Tissue culture supernatant
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> Regulation
Applications
Show applications key
Our Abpromise guarantee covers the use of ab40812 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/50,000Detects a band of approximately 95 kDa (predicted molecular weight: 95 kDa).
ICC
ICC: 1/50 - 1/100.ICC: 1/50 - 1/100.
Flow Cyt
Flow Cyt: 1/20Flow Cyt: 1/20
IP
IP: 1/50IP: 1/50
IHC-P: 1/100 - 1/250.
Target
Function
Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.
Sequence similarities
Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily.
Post-translational
modifications
Phosphorylation by EF-2 kinase completely inactivates EF-2.
Diphthamide is 2-[3-carboxyamido-3-(trimethyl-ammonio)propyl]histidine. Diphthamide can be ADP-ribosylated by diphtheria toxin and by Pseudomonas exotoxin A, thus arresting protein synthesis.
ISGylated.
Cellular localization
Cytoplasm.
Target information above from: UniProt accessionP13639
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- EEF 2 antibody
- Eef2 antibody
- EF-2 antibody
see all
Database links
- Entrez Gene: 1938 Human
- Entrez Gene: 13629 Mouse
- Entrez Gene: 29565 Rat
- Omim: 130610 Human
- SwissProt: P13639 Human
- SwissProt: P58252 Mouse
- SwissProt: P05197 Rat
- Unigene: 515070 Human
see all
Anti-EEF2 antibody images:
Western blot - EEF2 antibody (ab40812)
Anti-EEF2 antibody (ab40812) at 1/50000 dilution + 10ug HEK293
Predicted band size : 95 kDa
Observed band size : 95 kDa
Immunohistochemistry (Paraffin-embedded sections) - EEF2 antibody (ab40812)
ab40812 at a 1/100 dilution staining EEF2 in human breast carcinoma tissue by Immunohistochemistry, Paraffin embedded section.
References for Anti-EEF2 antibody (ab40812)
This product has been referenced in:
- Manadas Bet al. BDNF-induced changes in the expression of the translation machinery in hippocampal neurons: protein levels and dendritic mRNA. J Proteome Res 8:4536-52 (2009).Read more (PubMed: 19702335) »
See 1 publication for this product
Publishing research using ab40812? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"