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Anti-EEF2 (phospho T56) antibody (ab53114)

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Overview

Product name

Anti-EEF2 (phospho T56) antibody
See all EEF2 products (11) ...

Description

Rabbit polyclonal to EEF2 (phospho T56)

Specificity

ab53114 detects endogenous levels of EEF2 only when phosphorylated at threonine 56.

Tested applications

WB, ELISA, IHC-Pmore details

Cross reactivity

Reacts with

Mouse, Rat, Human

Immunogen

A synthetic peptide phosphopeptide derived from human EEF2 around the phosphorylation site of threonine 56 (R-F-TP-D-T).

Positive control

Human breast carcinoma tissue and extracts from NIH 3T3 cells treated with serum.

Properties

Form

Liquid

Storage instructions

Store at -20°C. Stable for 12 months at -20°C

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Purification notes

ab53114 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Clonality

Polyclonal

Isotype

IgG

Research areas

Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> Regulation

  • Western blot - EEF2 (phospho T56) antibody (ab53114)Western blot - EEF2 (phospho T56) antibody (ab53114) image (enlarge)

  • Immunohistochemistry (Paraffin-embedded sections) - EEF2 (phospho T56) antibody (ab53114)Immunohistochemistry (Paraffin-embedded sections) - EEF2 (phospho T56) antibody (ab53114) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab53114 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide1 Image

    WB

     WB: 1/300 - 1/1000.Detec...Read more →

    WB: 1/300 - 1/1000.Detects a band of approximately 95 kDa (predicted molecular weight: 95 kDa).

  • ELISA

     ELISA: 1/10000

    ELISA: 1/10000

  • 1 Image

    IHC-P

     IHC-P: 1/50 - 1/100.

    IHC-P: 1/50 - 1/100.

Target

Function

Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.

Sequence similarities

Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily.

Post-translational
modifications

Phosphorylation by EF-2 kinase completely inactivates EF-2.
Diphthamide is 2-[3-carboxyamido-3-(trimethyl-ammonio)propyl]histidine. Diphthamide can be ADP-ribosylated by diphtheria toxin and by Pseudomonas exotoxin A, thus arresting protein synthesis.
ISGylated.

Cellular localization

Cytoplasm.

Target information above from: UniProt accessionP13639 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • EEF 2 antibody
  • Eef2 antibody
  • EF-2 antibody
  • EF2 antibody
  • EF2_HUMAN antibody
  • Elongation factor 2 antibody
  • Eukaryotic translation elongation factor 2 antibody
  • Polypeptidyl tRNA translocase antibody
see all

Database links

see all

Anti-EEF2 (phospho T56) antibody images:

  Western blot - EEF2 (phospho T56) antibody (ab53114)

Western blot - EEF2 (phospho T56) antibody (ab53114)

All lanes : Anti-EEF2 (phospho T56) antibody (ab53114) at 1/300 dilution

Lane 1 : extract from NIH 3T3 cells treated with serum (10%, 30mins)
Lane 2 : extract from NIH 3T3 cells treated with serum (10%, 30mins) with immunising peptide


Predicted band size : 95 kDa
Observed band size : 95 kDa

  Immunohistochemistry (Paraffin-embedded sections) - EEF2 (phospho T56) antibody (ab53114)

Immunohistochemistry (Paraffin-embedded sections) - EEF2 (phospho T56) antibody (ab53114)

ab53114 at 1/50 dilution staining EEF2 in human breast carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence and presence of the immunising peptide.

References for Anti-EEF2 (phospho T56) antibody (ab53114)

ab53114 has not yet been referenced specifically in any publications.

Publishing research using ab53114? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"