EEF2 (phospho T56) antibody (ab53114)

Product Name 

EEF2 (phospho T56) antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to EEF2 (phospho T56)

Immunogen 

A synthetic peptide phosphopeptide derived from human EEF2 around the phosphorylation site of threonine 56 (R-F-T^P-D-T).

Reacts with 
(species key)

Hu, Ms, Rat

Specificity 

ab53114 detects endogenous levels of EEF2 only when phosphorylated at threonine 56.

Tested applications 
(see key)

ELISA, IHC-P, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
ELISA: 1/10000.
IHC-P: 1/50 - 1/100.
WB: 1/300 - 1/1000. Detects a band of approximately 95 kDa (predicted molecular weight: 95 kDa).

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Human breast carcinoma tissue and extracts from NIH 3T3 cells treated with serum.

Cellular localization 

Cytoplasmic

Research areas 

Chromatin and Nuclear Signaling >> DNA / RNA >> Translation >> Regulation

Relevance 

EEF2 is a member of the GTP-binding translation elongation factor family. This protein is an essential factor for protein synthesis. It promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. This protein is completely inactivated by EF-2 kinase phosporylation.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg²⁺ and Ca²⁺), 150mM Sodium chloride, pH 7.4

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Purification notes 

ab53114 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at -20°C. Stable for 12 months at -20°C

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