Overview

  • Product nameAnti-EEF2K antibody
    See all EEF2K primary antibodies
  • Description
    Rabbit polyclonal to EEF2K
  • Specificityab51213 detects endogenous levels of total EEF2K protein.
  • Tested applicationsWB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthesized non-phosphopeptide derived from human EEF2K around the phosphorylation site of serine 366 (T-L-SP-G-S).

  • Positive control
    • Extracts from Hela cells treated with serum

Properties

Applications

Our Abpromise guarantee covers the use of ab51213 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/300 - 1/1000. Detects a band of approximately 95 kDa (predicted molecular weight: 95 kDa).
ELISA 1/10000.

Target

  • FunctionPhosphorylates eukaryotic elongation factor-2. Binds calmodulin.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Alpha-type protein kinase family.
    Contains 1 alpha-type protein kinase domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • C86191 antibody
    • Calcium/calmodulin dependent eukaryotic elongation factor 2 antibody
    • Calcium/calmodulin dependent eukaryotic elongation factor 2 kinase antibody
    • Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase antibody
    • Calmodulin dependent protein kinase III antibody
    • cb365 antibody
    • EC 2.7.11.20 antibody
    • eEF 2 kinase antibody
    • eEF 2K antibody
    • eEF-2 kinase antibody
    • eEF-2K antibody
    • Eef2k antibody
    • EEF2K protein antibody
    • EF2K_HUMAN antibody
    • Elongation factor 2 kinase antibody
    • Eukaryotic elongation factor 2 kinase antibody
    • fa04b08 antibody
    • HSU93850 antibody
    • kinase eEF2K antibody
    • MGC45041 antibody
    • SMEF2K antibody
    • wu:fa04b08 antibody
    • zgc:100973 antibody
    see all

Anti-EEF2K antibody images

  • All lanes : Anti-EEF2K antibody (ab51213)

    Lane 1 : Extracts from Hela cells treated with serum (10%, 15mins) with no immunising peptide
    Lane 2 : Extracts from Hela cells treated with serum (10%, 15mins) with immunising peptide


    Predicted band size : 95 kDa

References for Anti-EEF2K antibody (ab51213)

ab51213 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Mouse Tissue lysate - whole (colon tumor)
Gel Running Conditions Non-reduced Non-Denaturing (Native)
Loading amount 30 µg
Specification colon tumor
Blocking step Milk as blocking agent for 45 minute(s) · Concentration: 5%
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Submitted Jul 02 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"