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- Secondary antibodies
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Product name
Anti-EEF2K antibody
See all EEF2K products (8) ...
Description
Rabbit polyclonal to EEF2K
Tested applications
WB, ICC/IFmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat
Immunogen
Recombinant fragment containing a sequence corresponding to a region within amino acids 1-238 of Human EEF2K (NP_037434).
Positive control
A431, H1299, HeLa and HepG2 whole cell lysates; A431 cells; Raji cell line
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calmodulin Pathway
Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> Regulation
Applications
Show applications key
Our Abpromise guarantee covers the use of ab96685 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/3000. Predicted molecular weight: 82 kDa.
ICC/IF: 1/100 - 1/200.
Target
Function
Phosphorylates eukaryotic elongation factor-2. Binds calmodulin.
Sequence similarities
Belongs to the protein kinase superfamily. Alpha-type protein kinase family.
Contains 1 alpha-type protein kinase domain.
Target information above from: UniProt accessionO00418
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- C86191 antibody
- Calcium/calmodulin dependent eukaryotic elongation factor 2 antibody
- Calcium/calmodulin dependent eukaryotic elongation factor 2 kinase antibody
see all
Database links
- Entrez Gene: 29904 Human
- Entrez Gene: 13631 Mouse
- Entrez Gene: 25435 Rat
- Omim: 606968 Human
- SwissProt: Q8N588 Human
- SwissProt: O00418 Human
- SwissProt: Q7TPC7 Mouse
- SwissProt: Q3UWZ1 Mouse
see all
Anti-EEF2K antibody images:
Western blot - EEF2K antibody (ab96685)
All lanes : Anti-EEF2K antibody (ab96685) at 1/5000 dilution
Lane 1 : A431 whole cell lysate
Lane 2 : H1299 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : HepG2 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size : 82 kDa
7.5% SDS-PAGE
Immunocytochemistry/ Immunofluorescence - EEF2K antibody (ab96685)
ab96685, at 1/200 dilution staining EEF2K in paraformaldehyde-fixed A431 cells by Immunofluorescence. Lower image is merged with DNA probe.
References for Anti-EEF2K antibody (ab96685)
ab96685 has not yet been referenced specifically in any publications.
Publishing research using ab96685? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"