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Product name
Anti-EEF2K (phospho S366) antibody
See all EEF2K products (8) ...
Description
Rabbit polyclonal to EEF2K (phospho S366)
Specificity
This antibody detects endogenous levels of EEF2K only when phosphorylated at serine 366.
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat
Immunogen
A synthesized phosphopeptide derived from human EEF2K around the phosphorylation site of serine 366 (T-L-SP-G-S).
Positive control
Hela cells.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calmodulin Pathway
Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> Regulation
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Western blot - EEF2K (phospho S366) antibody (ab51227)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab51227 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/300 - 1/1000. Detects a band of approximately 100 kDa (predicted molecular weight: 95 kDa).
ELISA
ELISA: 1/40000.ELISA: 1/40000.
Target
Function
Phosphorylates eukaryotic elongation factor-2. Binds calmodulin.
Sequence similarities
Belongs to the protein kinase superfamily. Alpha-type protein kinase family.
Contains 1 alpha-type protein kinase domain.
Target information above from: UniProt accessionO00418
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- C86191 antibody
- Calcium/calmodulin dependent eukaryotic elongation factor 2 antibody
- Calcium/calmodulin dependent eukaryotic elongation factor 2 kinase antibody
see all
Database links
- Entrez Gene: 29904 Human
- Entrez Gene: 13631 Mouse
- Entrez Gene: 25435 Rat
- Omim: 606968 Human
- SwissProt: Q8N588 Human
- SwissProt: O00418 Human
- SwissProt: Q7TPC7 Mouse
- SwissProt: Q3UWZ1 Mouse
see all
Anti-EEF2K (phospho S366) antibody images:
Western blot - EEF2K (phospho S366) antibody (ab51227)
All lanes : Anti-EEF2K (phospho S366) antibody (ab51227) at 1/300 dilution
Lane 1 : Extracts of Hela cells treated with 10% serum for 15mins and phosphopeptide.
Lane 2 : Extracts of Hela cells treated with 10% serum for 15mins.
Predicted band size : 95 kDa
Observed band size : 100 kDa (why is the actual band size different from the predicted?)
References for Anti-EEF2K (phospho S366) antibody (ab51227)
ab51227 has not yet been referenced specifically in any publications.
Publishing research using ab51227? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"