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Rabbit polyclonal to EFCAB5
antigen sequence, corresponding to amino acids 335-447 of Human EFCAB5.
- Human duodenum tissue; RT4, U251 MG, Human liver and tonsil lysates.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
Immunogen affinity purified
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
||1/250 - 1/500.
||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Contains 1 EF-hand domain.
Information by UniProt
- EF-hand calcium-binding domain-containing protein 5 antibody
- Efcab5 antibody
- EFCB5_HUMAN antibody
- FLJ46247 antibody
Immunocytochemistry/ Immunofluorescence - Anti-EFCAB5 antibody (ab121722)
Immunofluorescent staining of Human cell line U-2 OS shows positivity in mitochondria. Recommended concentration of ab121722 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EFCAB5 antibody (ab121722)
ab121722 at 1/75 dilution staining EFCAB5 in Paraffin-embedded Human duodenum tissue by Immunohistochemistry.
Western blot - Anti-EFCAB5 antibody (ab121722)
All lanes : Anti-EFCAB5 antibody (ab121722) at 1/250 dilution
Lane 1 : RT4 cell lysate
Lane 2 : U251 MG cell lysate
Lane 3 : Human plasma
Lane 4 : Human Liver tissue lysate
Lane 5 : Human Tonsil tissue lysate
Developed using the ECL technique
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"