EGF Human ELISA Kit (ab100504)
- Product nameEGF Human ELISA Kit
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Serum, Plasma
- Assay typeSandwich
- Sensitivity< 1 pg/ml
- Range0.82 pg/ml - 200 pg/ml
Sample specific recovery Sample type Average % Range Cell culture supernatant 95.52 85% - 106% Serum 94.52 83% - 104% Plasma 93.76 84% - 105%
- Species reactivityReacts with: Human
- Product overview
ab100504 Human EGF ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human EGF in serum, plasma and cell culture supernatants. This assay employs an antibody specific for Human EGF coated on a 96-well plate. Standards and samples are pipetted into the wells and EGF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human EGF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of EGF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimisation may be required with urine samples.
- Tested applicationsELISA more details
- Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 1 x 96 tests 20X Wash Buffer Concentrate Item B 1 x 25ml 600X HRP-Streptavidin Concentrate Item G 1 x 200µl Assay Diluent A diluent buffer, For Standard/Sample (serum/plasma) diluent. 1 x 30ml Assay Diluent B 5x concentrated buffer. For Standard/Sample (cell culture medium/urine) diluent. 1 x 15ml Detection Antibody EGF: biotinylated anti-human EGF 2 vials EGF Microplate (12 strips x 8 wells) coated with anti-human EGF 1 unit EGF Standard Human(recombinant) 2 vials Stop Solution Item I 1 x 8ml TMB One-Step Substrate Reagent Item H 1 x 12ml
- Research Areas
- Beta urogastroneEpidermal growth factorHOMG4
- OTTHUMP00000219721OTTHUMP00000219722Pro epidermal growth factorURGUrogastrone
- SwissProt: P01133 Human
Our Abpromise guarantee covers the use of ab100504 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
EGF Human ELISA Kit images
Levels of EGF were measured in Human dermal fibroblast (HDF), adipose-derived (ADM) and amniotic mesenchymal stem cells (AMM), using ab100504 EGF Human ELISA Kit.
Image from Kim SW et al., PLoS One. 2012;7(7):e41105. Epub 2012 Jul 17. Fig 3.; doi:10.1371/journal.pone.0041105; July 17, 2012, PLoS ONE 7(7): e41105.
References for EGF Human ELISA Kit (ab100504)
This product has been referenced in:
- Kim SW et al. Amniotic mesenchymal stem cells enhance wound healing in diabetic NOD/SCID mice through high angiogenic and engraftment capabilities. PLoS One 7:e41105 (2012). Human . Read more (PubMed: 22815931) »