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Overview

  • Product nameEGF Human ELISA Kit
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich
  • Sensitivity
    < 1 pg/ml
  • Range
    0.82 pg/ml - 200 pg/ml
  • Recovery

    95 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 95.52 85% - 106%
    Serum 94.52 83% - 104%
    Plasma 93.76 84% - 105%

  • Species reactivity
    Reacts with: Human
  • Product overview

    ab100504 Human EGF ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human EGF in serum, plasma and cell culture supernatants. This assay employs an antibody specific for Human EGF coated on a 96-well plate. Standards and samples are pipetted into the wells and EGF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human EGF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of EGF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimisation may be required with urine samples.

  • Tested applicationsELISA more details

Properties

  • RelevanceEpidermal growth factor (EGF) has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells. The EGF precursor is believed to exist as a membrane bound molecule which is proteolytically cleaved to generate the 53 amino acid peptide hormone that stimulates cells to divide. EGF exerts its actions by binding to the EGFR, a 170 kDa protein. Epidermal growth factor (EGF) is a key growth factor regulating cell survival. Through its binding to cell surface receptors, EGF activates an extensive network of signal transduction pathways that include activation of the PI3K/AKT, RAS/ERK and JAK/STAT pathways. Because of its key role in driving the proliferation of cells, EGFR is a target of several anti-cancer drugs currently in development.
    • Alternative names
        Beta urogastroneEpidermal growth factorHOMG4
        OTTHUMP00000219721OTTHUMP00000219722Pro epidermal growth factorURGUrogastrone
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab100504 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      ELISA ELISA

      EGF Human ELISA Kit images

      • ELISA - EGF Human ELISA Kit (ab100504)
        ELISA - EGF Human ELISA Kit (ab100504)
        Levels of EGF were measured in Human dermal fibroblast (HDF), adipose-derived (ADM) and amniotic mesenchymal stem cells (AMM), using ab100504 EGF Human ELISA Kit.

        Image from Kim SW et al., PLoS One. 2012;7(7):e41105. Epub 2012 Jul 17. Fig 3.; doi:10.1371/journal.pone.0041105; July 17, 2012, PLoS ONE 7(7): e41105.
      • ELISA - EGF Human ELISA Kit (ab100504)
        ELISA - EGF Human ELISA Kit (ab100504)

      Protocols

      References for EGF Human ELISA Kit (ab100504)

      This product has been referenced in:
      • Kim SW  et al. Amniotic mesenchymal stem cells enhance wound healing in diabetic NOD/SCID mice through high angiogenic and engraftment capabilities. PLoS One 7:e41105 (2012). Human . Read more (PubMed: 22815931) »

      See 1 Publication for this product

      Publishing research using ab100504 ? Please let us know so that we can cite the reference in this datasheet

      Product Wall

      Submit an Abreview for ab100504 Submit a Question for ab100504

      Displaying 1 - 9 of 9 results for Abreviews and Q&A

      10
      Votes: Highest First

      We purchased an ELISA kit for EGF (ab100504) a month ago (arrived 28th Sep 2012). I recently used the kit with the provided standards and my samples only to find that there was absolutely no signal whatsoever including the standard wells. I have in the pa...

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      I am sorry to read that the most recent order of the EGF ELISA gave a poor result compared to previous kits. I have arranged for a free replacement from a different lot, order ***.

      To check the status of the order please contact our Customer Se...

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      The ELISA kit you sent to me as a replacement for one of the kits worked fine. The only thing I noticed was that, the assay diluent B and wash buffer had crystallised and to resuspend it had to be sonicated for 45 min.
      Could you please arrange to rep...

      Read More

      Thank you for confirming these details and for your update. I am pleased to hear the FOCR kit has worked well. This enquiry has been forwarded to me as my colleague #### is currently away from the office.

      As requested, I have now issued the sec...

      Read More

      Hi,

      That sounds perfectly reasonable. We are not closed for Easter at UC Davis. So you could send it at the earliest and we should be able to receive the kit.

      If you could send the kit to :


      Looking forward to it.

      Read More

      Thank you for confirming that information.

      I have now arranged for the kit to be sent out to you. The order number is xxxxx(Purchase order number FOCR xxxxx).

      I hope this kit will work as your previous ones have, however, if you have a...

      Read More

      Hi

      Many thanks for the response. The kit I used contained ONLY 8 uL of the 30,000 HRP-streptavidin unlike the 200 uL you suggest it may have had, since it had only 8 uL I went for the 30,000 dilution as with the old protocol.

      Thank you for providing me with that information.

      Could you please check how much HRP-streptavidin is in the unused kit which you have? Also, for both of these kits, what dilution did the protocol sent witheach kit advise?

      Many thanks ...

      Read More

      Hi


      The lot number for the kit that worked was GR64822-1.


      Thanks



      We may have identified what the difference may be. Could you confirm, how did you dilute the HRP-streptavidin concentrate provided with the kit?

      We have recently modified this reagent and the old kit contained 8 uL of 30,000x HRP-strep...

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      Hi

      1) The standards and the kit were stored as per Abcam/kit instructions. We've not had a problem with the EGF kits we purchased prior to this. This was very specific to this particular batch of kit. The standards were reconsituted immediately...

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      Thank you for providing that information.

      I am sorry that it seems that this kit is not performing as expected. It would be helpful for me if you could provide me with the lot numbers or order numbers of the kits which you have found to work wit...

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      Dear tech support
      We recently purcahsed ELISA kits for human EGF ab100504. While doing an
      assay on one of the kits that we ran into a hiccup despite following the
      protocol to the point. The standard curve did not work well with black
      p...

      Read More

      Thank you for contacting us and reporting the problems you have been encountering with the EGF Human ELISA Kit (ab100504). We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

      If ...

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      Hi I recently purchased the EGF human ELISA kit (ab100504). While I am aware that the kit is intended for detection of EGF in human samples, I was wondering if you knews the cross reactivity of the assay in other species such as Bovine....

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      Thank you for your enquiry. I am sorry to confirm that  Bovine samples have not been tested and guaranteed with the ab100504 EGF Human ELISA Kit,  so there is no cross reactivity data.  Epitope mapping was also not performed so we do not know exactly ...

      Read More

      Could this kit cross-react with bovine protein?

      Thanks for your call tyesterday and for your patience while I have been in touch with the lab. I have heard back about your enquiry. Bovine samples have not been tested with the kit so we do not have any cross-reactivity data to conclusively determine thi...

      Read More

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"