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Product name
Anti-EGF antibody [144-8]
See all EGF products (16) ...
Description
Mouse monoclonal [144-8] to EGF
Specificity
ab54422 shows no cross reactivity with either human or rat transforming growth factor-alpha (TGF-alpha).
Tested applications
IP, IHC-Frmore details
Cross reactivity
Reacts with
Human
Immunogen
Full length native protein (Human) purified from urine.
Positive control
Human salivary gland tissue.
General notes
Does not inhibit the binding of EGF to the EGF receptor. The approximate affinity constant of the antibody for EGF is 0.1 nM.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 50% Glycerol, 50mM Sodium phosphate
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Clone number
144-8
Myeloma
P3x63-Ag8.653
Isotype
IgG1
Research areas
Cancer >> Growth factors >> EGF
Signal Transduction >> Growth Factors/Hormones >> EGF
Applications
Show applications key
Our Abpromise guarantee covers the use of ab54422 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP
IHC-Fr
Application notes
IHC-Fr: Use at a concentration of 1 - 5 µg/ml.
IP: Use at a concentration of 1 - 2 µg/sample.
Is unsuitable for Neut.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6.
Tissue specificity
Expressed in kidney, salivary gland, cerebrum and prostate.
Involvement in disease
Defects in EGF are the cause of hypomagnesemia type 4 (HOMG4) [MIM:611718]; also known as renal hypomagnesemia normocalciuric. HOMG4 is a disorder characterized by massive renal hypomagnesemia and normal levels of serum calcium and calcium excretion. Clinical features include seizures, mild-to mederate psychomotor retardation, and brisk tendon reflexes.
Sequence similarities
Contains 9 EGF-like domains.
Contains 9 LDL-receptor class B repeats.
Cellular localization
Membrane.
Target information above from: UniProt accessionP01133
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Beta urogastrone antibody
- EGF antibody
- Egf antibody
see all
Database links
- Entrez Gene: 1950 Human
- Omim: 131530 Human
- SwissProt: P01133 Human
- Unigene: 419815 Human
References for Anti-EGF antibody [144-8] (ab54422)
ab54422 has not yet been referenced specifically in any publications.
Publishing research using ab54422? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"