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Overview

  • Product nameAnti-EGF antibody (Biotin)See all EGF primary antibodies ...
  • Description
    Rabbit polyclonal to EGF (Biotin)
  • ConjugationBiotin
  • Tested applicationsWB, ELISA, Sandwich ELISA more details
  • Species reactivity
    Reacts with: Rat
  • Immunogen

    Recombinant full length EGF protein (Rat) highly pure (>98%).

  • General notesA sterile filtered antibody solution was lyophilized.

Properties

  • FormLyophilised:Centrifuge vial prior to opening. Reconstitute in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
  • Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab84253 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 134 kDa.
ELISA ELISA: Use a concentration of 0.25 - 1 µg/ml.
Sandwich ELISA sELISA: Use a concentration of 0.25 - 1 µg/ml.

Target

  • FunctionEGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6.
  • Tissue specificityExpressed in kidney, salivary gland, cerebrum and prostate.
  • Involvement in diseaseDefects in EGF are the cause of hypomagnesemia type 4 (HOMG4) [MIM:611718]; also known as renal hypomagnesemia normocalciuric. HOMG4 is a disorder characterized by massive renal hypomagnesemia and normal levels of serum calcium and calcium excretion. Clinical features include seizures, mild-to mederate psychomotor retardation, and brisk tendon reflexes.
  • Sequence similaritiesContains 9 EGF-like domains.
    Contains 9 LDL-receptor class B repeats.
  • Cellular localizationMembrane.

    • Target information above from: UniProt accession P01133 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    • Alternative names
        Beta urogastrone antibodyEGF antibodyEgf antibody
        EGF_HUMAN antibodyEpidermal Growth Factor antibodyHOMG4 antibodyOTTHUMP00000219721 antibodyOTTHUMP00000219722 antibodyPro epidermal growth factor antibodyURG antibodyUrogastrone antibody
      see all

    References for Anti-EGF antibody (Biotin) (ab84253)

    ab84253 has not yet been referenced specifically in any publications.

    Publishing research using ab84253 ? Please let us know so that we can cite the reference in this datasheet

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"