EGF antibody [EGF-10] (ab10409)

Product Name 

EGF antibody [EGF-10]

Product type 

Primary antibodies

Description 

Mouse monoclonal [EGF-10] to EGF

Immunogen 

Recombinant full length protein (Human).

Reacts with 

Hu

Does not react with 
(species key)

Ms

Specificity 

Ab10409 was tested against recombinant EGF, Abcam therefore cannot guarantee it will be suitable against endogenous EGF in WB.

Tested applications 
(see key)

Dot, ELISA, IHC-P, RIA

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
Dot: Use at a concentration of 0.1 µg/ml to detect 25ng protein.
ELISA: Use at an assay dependent dilution.
IHC-P: 1/500 (see Abreview).
RIA: Use at an assay dependent dilution.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Cell Membrane, single-pass type I membrane protein [precursor] and Secreted [EGF].

Research areas 

Cancer >> Growth factors >> EGF
Signal Transduction >> Growth Factors/Hormones >> EGF

Relevance 

Epidermal growth factor (EGF) has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide. EGF exerts its actions by binding to the EGFR, a 170 kDa protein.
Epidermal growth factor (EGF) is a key growth factor regulating cell survival. Through its binding to cell surface receptors, EGF activates an extensive network of signal transduction pathways that include activation of the PI3K/AKT, RAS/ERK and JAK/STAT pathways. Because of its key role in driving the proliferation of cells, EGFR is a target of several anti-cancer drugs currently in development.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

EGF-10

Isotype 

IgG1

Purity 

Protein A purified

Storage buffer 

Preservative: 0.1% Sodium Azide
Constituents: PBS

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

Batch dependent within range: 2.00 - 2.30 mg/ml

Concentration of lot no.     is  

Concentration not available for this lot.

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Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Notes 

If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

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