You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Reassurance, Refunds & Replacements

If your product does not perform as described on this datasheet, we will refund or replace your product...

Read our guarantee »
 

Anti-EGF antibody [EGF-10] (ab10409)

CodeSizePriceAbpointsAvailability
    
Add to basket
 
  • -
  •   
  •   
  •   
  •  
  •   
  •   
Updating...

Overview

Product name

Anti-EGF antibody [EGF-10]
See all EGF products (16) ...

Description

Mouse monoclonal [EGF-10] to EGF

Specificity

Ab10409 was tested against recombinant EGF, Abcam therefore cannot guarantee it will be suitable against endogenous EGF in WB.

Tested applications

Dot Blot, IHC-Fr, ELISA, RIA, IHC-Pmore details

Cross reactivity

Reacts with

Human

Does not react with

Mouse

Immunogen

Recombinant full length protein (Human).

General notes

If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: None
Constituents: 0.01M PBS, pH 7.4

Concentration

Concentration information loading...

Purity

Protein A purified

Clonality

Monoclonal

Clone number

EGF-10

Isotype

IgG1

Research areas

Cancer >> Growth factors >> EGF
Signal Transduction >> Growth Factors/Hormones >> EGF

  • Immunohistochemistry (Frozen sections) - EGF antibody [EGF-10] (ab10409)Immunohistochemistry (Frozen sections) - EGF antibody [EGF-10] (ab10409) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab10409 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6.

Tissue specificity

Expressed in kidney, salivary gland, cerebrum and prostate.

Involvement in disease

Defects in EGF are the cause of hypomagnesemia type 4 (HOMG4) [MIM:611718]; also known as renal hypomagnesemia normocalciuric. HOMG4 is a disorder characterized by massive renal hypomagnesemia and normal levels of serum calcium and calcium excretion. Clinical features include seizures, mild-to mederate psychomotor retardation, and brisk tendon reflexes.

Sequence similarities

Contains 9 EGF-like domains.
Contains 9 LDL-receptor class B repeats.

Cellular localization

Membrane.

Target information above from: UniProt accessionP01133 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Beta urogastrone antibody
  • EGF antibody
  • Egf antibody
  • EGF_HUMAN antibody
  • Epidermal Growth Factor antibody
  • Epidermal Growth Factor antibody
  • HOMG4 antibody
  • OTTHUMP00000219721 antibody
  • OTTHUMP00000219722 antibody
  • Pro epidermal growth factor antibody
  • URG antibody
  • Urogastrone antibody
see all

Database links

Anti-EGF antibody [EGF-10] images:

  Immunohistochemistry (Frozen sections) - EGF antibody [EGF-10] (ab10409)

Immunohistochemistry (Frozen sections) - EGF antibody [EGF-10] (ab10409)

ab10409 staining EGF in human normal skin tissue sections by Immunohistochemistry (frozen sections). Tissue was fixed with formaldehyde. Samples were blocked with 10% serum for 30 minutes at 21°C followed by incubation with the primary antibody at a 1/500 dilution for 30 minutes at 21°C. An undiluted HRP-conjugated goat polyclonal was used as the secondary antibody.

Image courtesy of an anonymous Abreview.

See Abreview

References for Anti-EGF antibody [EGF-10] (ab10409)

This product has been referenced in:

  • Lindenmeyer MTet al. Interstitial vascular rarefaction and reduced VEGF-A expression in human diabetic nephropathy. J Am Soc Nephrol 18:1765-76 (2007). IHC-P, IHC-Fr; Human.Read more (PubMed: 17475821) »

See 1 publication for this product

Publishing research using ab10409? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"