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Anti-EGF antibody [EGF-2] (ab8282)

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Overview

Product name

Anti-EGF antibody [EGF-2]
See all EGF products (16) ...

Description

Mouse monoclonal [EGF-2] to EGF

Tested applications

WB, ICC/IFmore details

Cross reactivity

Reacts with

Human

Immunogen

Full length native protein (purified) (Human).

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

PBS with 0.1% sodium azide, pH 7.4

Concentration

Concentration information loading...

Purity

Protein G purified

Clonality

Monoclonal

Clone number

EGF-2

Myeloma

Sp2/0

Isotype

IgG1

Light chain type

unknown

Research areas

Cancer >> Growth factors >> EGF
Signal Transduction >> Growth Factors/Hormones >> EGF

  • Immunocytochemistry/ Immunofluorescence - EGF antibody [EGF-2] (ab8282)Immunocytochemistry/ Immunofluorescence - EGF antibody [EGF-2] (ab8282) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab8282 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

ICC/IF: Use at a concentration of 5 µg/ml.
WB: Use at an assay dependent concentration.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6.

Tissue specificity

Expressed in kidney, salivary gland, cerebrum and prostate.

Involvement in disease

Defects in EGF are the cause of hypomagnesemia type 4 (HOMG4) [MIM:611718]; also known as renal hypomagnesemia normocalciuric. HOMG4 is a disorder characterized by massive renal hypomagnesemia and normal levels of serum calcium and calcium excretion. Clinical features include seizures, mild-to mederate psychomotor retardation, and brisk tendon reflexes.

Sequence similarities

Contains 9 EGF-like domains.
Contains 9 LDL-receptor class B repeats.

Cellular localization

Membrane.

Target information above from: UniProt accessionP01133 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Beta urogastrone antibody
  • EGF antibody
  • Egf antibody
  • EGF_HUMAN antibody
  • Epidermal Growth Factor antibody
  • Epidermal Growth Factor antibody
  • HOMG4 antibody
  • OTTHUMP00000219721 antibody
  • OTTHUMP00000219722 antibody
  • Pro epidermal growth factor antibody
  • URG antibody
  • Urogastrone antibody
see all

Database links

Anti-EGF antibody [EGF-2] images:

  Immunocytochemistry/ Immunofluorescence - EGF antibody [EGF-2] (ab8282)

Immunocytochemistry/ Immunofluorescence - EGF antibody [EGF-2] (ab8282)

ICC/IF image of ab8282 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab8282, 5µg/ml) overnight at +4°C. The secondary antibody (green)ÿwas Alexa Fluor© 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor© 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-EGF antibody [EGF-2] (ab8282)

ab8282 has not yet been referenced specifically in any publications.

Publishing research using ab8282? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"