Anti-EGF antibody [S-147] (ab18632)

Overview

• Product nameAnti-EGF antibody [S-147]See all EGF primary antibodies ...
• Description
  Mouse monoclonal [S-147] to EGF
• SpecificityReactive with natural and recombinant Human EGF but does not react with other human cytokines tested (IL 1beta, IL 8, MCAF, TGF beta and SAA).
• Tested applicationsELISA more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Purified recombinant human EGF. 6kDA, 53 amino acid residues

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: None
  Constituents: 0.01M PBS, pH 7.2
• Concentration information loading...
• PurityProtein G purified
• Clonality Monoclonal
• Clone numberS-147
• MyelomaSp2/0-Ag14
• IsotypeIgG1
• Light chain typekappa
• Research Areas
  • Cancer
  • Growth factors
  • EGF

  • Signal Transduction
  • Growth Factors/Hormones
  • EGF

Applications

Target

• FunctionEGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6.
• Tissue specificityExpressed in kidney, salivary gland, cerebrum and prostate.
• Involvement in diseaseDefects in EGF are the cause of hypomagnesemia type 4 (HOMG4) [MIM:611718]; also known as renal hypomagnesemia normocalciuric. HOMG4 is a disorder characterized by massive renal hypomagnesemia and normal levels of serum calcium and calcium excretion. Clinical features include seizures, mild-to mederate psychomotor retardation, and brisk tendon reflexes.
• Sequence similaritiesContains 9 EGF-like domains.
  Contains 9 LDL-receptor class B repeats.
• Cellular localizationMembrane.

Target information above from: UniProt accession P01133 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 1950 Human
  • Omim: 131530 Human
  • SwissProt: P01133 Human
  • Unigene: 419815 Human

• Alternative names
  Beta urogastrone antibodyEGF antibodyEgf antibody

  EGF_HUMAN antibodyEpidermal Growth Factor antibodyEpidermal Growth Factor antibodyHOMG4 antibodyOTTHUMP00000219721 antibodyOTTHUMP00000219722 antibodyPro epidermal growth factor antibodyURG antibodyUrogastrone antibody

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Anti-EGF antibody [S-147] images

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  Immunocytochemistry/ Immunofluorescence-EGF antibody [S-147](ab18632)
  ICC/IF image of ab18632 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab18632, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.