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Product name
Anti-EGF antibody
See all EGF products (16) ...
Description
Rabbit polyclonal to EGF
Tested applications
ELISA, WB, Indirect ELISAmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant Human EGF
Properties
Form
Lyophilised:
Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
Storage instructions
Store at -20°C, Stable for 6 months at -20°C
Storage buffer
Preservative: None
Constituents: PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
ab94898 was purified by affinity chromatography employing immobilized Human EGF matrix
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Growth factors >> EGF
Signal Transduction >> Growth Factors/Hormones >> EGF
Applications
Show applications key
Our Abpromise guarantee covers the use of ab94898 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
ELISA: 1/0.5 - 1/2.ELISA: 1/0.5 - 1/2.
WB: Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 134 kDa.Detection limit is 1.5-3.0 ng, under either reducing or non-reducing conditions
I-ELISA: Use a concentration of 0.5 - 2 µg/ml. To detect IL-10 by indirect ELISA (using 100 µl/well antibody solution) a concentration of 0.5 - 2.0 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant rIL-10.
Target
Function
EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6.
Tissue specificity
Expressed in kidney, salivary gland, cerebrum and prostate.
Involvement in disease
Defects in EGF are the cause of hypomagnesemia type 4 (HOMG4) [MIM:611718]; also known as renal hypomagnesemia normocalciuric. HOMG4 is a disorder characterized by massive renal hypomagnesemia and normal levels of serum calcium and calcium excretion. Clinical features include seizures, mild-to mederate psychomotor retardation, and brisk tendon reflexes.
Sequence similarities
Contains 9 EGF-like domains.
Contains 9 LDL-receptor class B repeats.
Cellular localization
Membrane.
Target information above from: UniProt accessionP01133
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Beta urogastrone antibody
- EGF antibody
- Egf antibody
see all
Database links
- Entrez Gene: 1950 Human
- Omim: 131530 Human
- SwissProt: P01133 Human
- Unigene: 419815 Human
References for Anti-EGF antibody (ab94898)
ab94898 has not yet been referenced specifically in any publications.
Publishing research using ab94898? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"