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Products:Signal Transduction >> Growth Factors/Hormones >> EGF
Overview
Product name
Anti-EGF antibody
See all EGF products (16) ...
Description
Rabbit polyclonal to EGF
Tested applications
WB, ELISA, Neutralising, Sandwich ELISA, IHC-Pmore details
Cross reactivity
Reacts with
Human
Immunogen
Highly pure (>98%) recombinant hEGF (human Epidermal Growth Factor)
Properties
Form
Lyophilised:
Reconstitute with 200µl of sterile water.
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
PBS, pH 7.4, no preservative, sterile filtered
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
unknown
Light chain type
unknown
Research areas
Cancer >> Growth factors >> EGF
Signal Transduction >> Growth Factors/Hormones >> EGF
Applications
Show applications key
Our Abpromise guarantee covers the use of ab9695 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 0.1 - 0.2 µg/ml.(To detect hEGF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hEGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Western blot bands should be around 6.2 kDa.)
ELISA: Use a concentration of 0.5 µg/ml(To detect hEGF by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hEGF.)
Neut: Use a concentration of 0.25 - 0.35 µg/ml.(To yield one-half maximal inhibition [ND50] of the biological activity of hEGF (2.0 ng/ml), a concentration of 0.25 - 0.35 µg/ml of this antibody is required.)
sELISA: Use a concentration of 0.5 µg/ml Can be paired for Sandwich ELISA with Mouse monoclonal [S-177] to EGF (ab18635). (For sandwich ELISA, use this antibody as Detection at 0.5µg/ml with Ab18635 as Capture.)
IHC-P: Use a concentration of 1 µg/ml
Target
Function
EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6.
Tissue specificity
Expressed in kidney, salivary gland, cerebrum and prostate.
Involvement in disease
Defects in EGF are the cause of hypomagnesemia type 4 (HOMG4) [MIM:611718]; also known as renal hypomagnesemia normocalciuric. HOMG4 is a disorder characterized by massive renal hypomagnesemia and normal levels of serum calcium and calcium excretion. Clinical features include seizures, mild-to mederate psychomotor retardation, and brisk tendon reflexes.
Sequence similarities
Contains 9 EGF-like domains.
Contains 9 LDL-receptor class B repeats.
Cellular localization
Membrane.
Target information above from: UniProt accessionP01133
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Beta urogastrone antibody
- EGF antibody
- Egf antibody
see all
Database links
- Entrez Gene: 1950 Human
- Omim: 131530 Human
- SwissProt: P01133 Human
- Unigene: 419815 Human
Anti-EGF antibody images:
Western blot - Anti-EGF antibody (ab9695)
All lanes : Anti-EGF antibody (ab9695) at 0.2 µg/ml
Lane 1 : EGF protein (ab55483) at 0.1 µg
Lane 2 : EGF protein (ab55483) at 0.01 µg
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Exposure time : 1 minute
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - EGF antibody (ab9695)
ab9695 at 1.0 µg/ml staining normal human skin by IHC-P. The antibody was incubated overnight at 4˚C. An HRP-labeled polymer detection system was used with a non-alcohol soluble AEC chromogen and a proteinase K antigen retrieval.
Sandwich ELISA - EGF antibody (ab9695)
Standard Curve for EGF (Analyte: ab51082) dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [S-177] to EGF (ab18635) at 5ug/ml and Detector Antibody Rabbit polyclonal to EGF (ab9695) at 0.5ug/ml
References for Anti-EGF antibody (ab9695)
This product has been referenced in:
- Kumagai Ket al. Up-regulation of EGF receptor and its ligands, AREG, EREG, and HB-EGF in oral lichen planus. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 110:748-54 (2010). IHC-P; Human.Read more (PubMed: 20952227) »
See 1 publication for this product
Publishing research using ab9695? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"