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Anti-EGF antibody (ab9862)

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Overview

Product name

Anti-EGF antibody
See all EGF products (16) ...

Description

Goat polyclonal to EGF

Tested applications

ELISA, WBmore details

Cross reactivity

Reacts with

Mouse

Immunogen

Highly pure (>98%) recombinant mEGF (mouse Epidermal Growth Factor)

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

Storage buffer

PBS, pH 7.4, no preservative, sterile filtered

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Clonality

Polyclonal

Isotype

unknown

Light chain type

unknown

Research areas

Cancer >> Growth factors >> EGF
Signal Transduction >> Growth Factors/Hormones >> EGF

Applications

Show applications key

Our Abpromise guarantee covers the use of ab9862 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ELISA

     

  • WB

     

Application notes

ELISA:To detect mEGF by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mEGF.

Western Blot:To detect mEGF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mEGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Target

Function

EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6.

Tissue specificity

Expressed in kidney, salivary gland, cerebrum and prostate.

Involvement in disease

Defects in EGF are the cause of hypomagnesemia type 4 (HOMG4) [MIM:611718]; also known as renal hypomagnesemia normocalciuric. HOMG4 is a disorder characterized by massive renal hypomagnesemia and normal levels of serum calcium and calcium excretion. Clinical features include seizures, mild-to mederate psychomotor retardation, and brisk tendon reflexes.

Sequence similarities

Contains 9 EGF-like domains.
Contains 9 LDL-receptor class B repeats.

Cellular localization

Membrane.

Target information above from: UniProt accessionP01133 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Beta urogastrone antibody
  • EGF antibody
  • Egf antibody
  • EGF_HUMAN antibody
  • Epidermal Growth Factor antibody
  • Epidermal Growth Factor antibody
  • HOMG4 antibody
  • OTTHUMP00000219721 antibody
  • OTTHUMP00000219722 antibody
  • Pro epidermal growth factor antibody
  • URG antibody
  • Urogastrone antibody
see all

Database links

References for Anti-EGF antibody (ab9862)

ab9862 has not yet been referenced specifically in any publications.

Publishing research using ab9862? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"