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If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab9697? Please let us know so that we can cite the reference in this datasheet
ab9697 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - EGF protein (Active) (ab9697)
Lane 1 :
Lane 2 :
Lane 3 :
Lane 1 : Whole cell lysate of human skin fibroblasts starved overnight in serum-free medium
Lane 2 : Whole cell lysate of human skin fibroblasts starved overnight in serum-free medium and then incubated for 30 min with 25 ng/ml active EGF
Lane 3 : Whole cell lysate of human skin fibroblasts starved overnight in serum-free medium and then incubated for 30 min with 50 ng/ml active EGF
Lysates/proteins at 20 µg per lane.
Secondary
An HRP-conjugatede Goat anti-rabbit IgG polyclonal at 1/10000 dilution
developed using the ECL technique
Performed under non-reducing conditions.
Observed band size : 42,44 kDa (why is the actual band size different from the predicted?)
Exposure time : 1 second
Blocking Step: 5% milk for 1 hour at 25°C
This image is courtesy of an anonymous Abreview
EGF protein (Active) for Western blot in Human (9697)
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