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If your product does not perform as described on this datasheet, we will refund or replace your product...
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Western blot - EGF protein (Active) (ab9697)
Lane 1 :
Lane 2 :
Lane 3 :
Lane 1 : Whole cell lysate of human skin fibroblasts starved overnight in serum-free medium
Lane 2 : Whole cell lysate of human skin fibroblasts starved overnight in serum-free medium and then incubated for 30 min with 25 ng/ml active EGF
Lane 3 : Whole cell lysate of human skin fibroblasts starved overnight in serum-free medium and then incubated for 30 min with 50 ng/ml active EGF
Lysates/proteins at 20 µg per lane.
Secondary
An HRP-conjugatede Goat anti-rabbit IgG polyclonal at 1/10000 dilution
developed using the ECL technique
Performed under non-reducing conditions.
Observed band size : 42,44 kDa (why is the actual band size different from the predicted?)
Exposure time : 1 second
Blocking Step: 5% milk for 1 hour at 25°C
This image is courtesy of an anonymous Abreview
EGF protein (Active) for Western blot in Human (9697)
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