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Products:Signal Transduction >> Metabolism >> EGF
Overview
Product name
EGF protein (Active)
See all EGF products (8) ...
Protein description
Recombinant full length protein (Human)
Expression host
E. coli
Properties
Purification notes
Sterile filtered Greater than 98% pure by SDS-PAGE and HPLC analyses. Endotoxin level is less than 0.1 ng per µg (1EU/µg).
Form
Lyophilised:
Centrifuge the vial prior to opening. Reconstitute in 500ul sterile filtered water to a concentration of 1mg/ml.
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Concentration
Concentration information loading...
Additional notes
Manufactured using all non-animal reagents.
Sequence notes
Recombinan t Human EGF is a 6.2 kDa protein containing 53 amino acid residues: NSDSECP LSH DGYCLHDGVC MYIEALDKYA CNCVVGYIGE RCQYRDLKWW ELR
Research areas
Stem Cells >> Stem Cell Cytokines >> Recombinant Human
Cancer >> Growth factors >> EGF
Signal Transduction >> Growth Factors/Hormones >> EGF
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Western blot - EGF protein (Active) (ab9697)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab9697 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at an assay dependent dilution.
CellAct: Use at an assay dependent dilution. (The ED50, determined by the dose-dependent stimulation of the proliferation of thymidine uptake by BALB/c 3T3 cells, is < 0.1 ng/ml, corresponding to a specific activity of > 1 x 107 units/mg)
SDS-PAGE: Use at an assay dependent dilution.
Application notes
This peptide can be used with studies using ab9695.
Protein info
Function
EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6.
Tissue specificity
Expressed in kidney, salivary gland, cerebrum and prostate.
Involvement in disease
Defects in EGF are the cause of hypomagnesemia type 4 (HOMG4) [MIM:611718]; also known as renal hypomagnesemia normocalciuric. HOMG4 is a disorder characterized by massive renal hypomagnesemia and normal levels of serum calcium and calcium excretion. Clinical features include seizures, mild-to mederate psychomotor retardation, and brisk tendon reflexes.
Sequence similarities
Contains 9 EGF-like domains.
Contains 9 LDL-receptor class B repeats.
Cellular localization
Membrane.
Target information above from: UniProt accessionP01133
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
EGF protein (Active) images:
Western blot - EGF protein (Active) (ab9697)
Lane 1 :
Lane 2 :
Lane 3 :
Lane 1 : Whole cell lysate of human skin fibroblasts starved overnight in serum-free medium
Lane 2 : Whole cell lysate of human skin fibroblasts starved overnight in serum-free medium and then incubated for 30 min with 25 ng/ml active EGF
Lane 3 : Whole cell lysate of human skin fibroblasts starved overnight in serum-free medium and then incubated for 30 min with 50 ng/ml active EGF
Lysates/proteins at 20 µg per lane.
Secondary
An HRP-conjugatede Goat anti-rabbit IgG polyclonal at 1/10000 dilution
developed using the ECL technique
Performed under non-reducing conditions.
Observed band size : 42,44 kDa (why is the actual band size different from the predicted?)
Exposure time : 1 second
Blocking Step: 5% milk for 1 hour at 25°C
This image is courtesy of an anonymous Abreview
References for EGF protein (Active) (ab9697)
ab9697 has not yet been referenced specifically in any publications.
Publishing research using ab9697? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"