You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

EGF protein (Active) (ab9697)

Abreviews (1)Q&A (1)

Overview

  • Product nameEGF protein (Active)See all EGF proteins and peptides ...
  • Protein descriptionRecombinant full length protein (Human)
  • Expression hostE. coli

    • Properties

  • Purification notesSterile filtered Greater than 98% pure by SDS-PAGE and HPLC analyses. Endotoxin level is less than 0.1 ng per µg (1EU/µg).
  • FormLyophilised:Centrifuge the vial prior to opening. Reconstitute in 500ul sterile filtered water to a concentration of 1mg/ml.
  • Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.This product is an active protein and may elicit a biological response in vivo, handle with caution.
  • Concentration information loading...
  • Additional notesManufactured using all non-animal reagents.
  • Sequence notesRecombinant Human EGF is a 6.2 kDa protein containing 53 amino acid residues: NSDSECPLSH DGYCLHDGVC MYIEALDKYA CNCVVGYIGE RCQYRDLKWW ELR
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab9697 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    WB WB: Use at an assay dependent dilution.
    Cellular activation CellAct: Use at an assay dependent dilution. The ED50, determined by the dose-dependent stimulation of the proliferation of thymidine uptake by BALB/c 3T3 cells, is < 0.1 ng/ml, corresponding to a specific activity of > 1 x 107 units/mg
    SDS-PAGE SDS-PAGE: Use at an assay dependent dilution.
  • Application notesThis peptide can be used with studies using ab9695,ab155576 and ab171327.

    • Protein info

    • Alternative names
        Beta urogastroneEGFEgf
        EGF_HUMANEpidermal Growth FactorEpidermal Growth FactorHOMG4OTTHUMP00000219721OTTHUMP00000219722Pro epidermal growth factorURGUrogastrone
      see all
  • FunctionEGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6.
  • Tissue specificityExpressed in kidney, salivary gland, cerebrum and prostate.
  • Involvement in diseaseDefects in EGF are the cause of hypomagnesemia type 4 (HOMG4) [MIM:611718]; also known as renal hypomagnesemia normocalciuric. HOMG4 is a disorder characterized by massive renal hypomagnesemia and normal levels of serum calcium and calcium excretion. Clinical features include seizures, mild-to mederate psychomotor retardation, and brisk tendon reflexes.
  • Sequence similaritiesContains 9 EGF-like domains.
    Contains 9 LDL-receptor class B repeats.
  • Cellular localizationMembrane.

    • Target information above from: UniProt accession P01133 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    EGF protein (Active) images

    • Western blot - EGF protein (Active) (ab9697)
      Western blot - EGF protein (Active) (ab9697)
      Lane 1 :
      Lane 2 :
      Lane 3 :

      Lane 1 : Whole cell lysate of human skin fibroblasts starved overnight in serum-free medium
      Lane 2 : Whole cell lysate of human skin fibroblasts starved overnight in serum-free medium and then incubated for 30 min with 25 ng/ml active EGF
      Lane 3 : Whole cell lysate of human skin fibroblasts starved overnight in serum-free medium and then incubated for 30 min with 50 ng/ml active EGF

      Lysates/proteins at 20 µg per lane.

      Secondary
      An HRP-conjugatede Goat anti-rabbit IgG polyclonal at 1/10000 dilution
      developed using the ECL technique

      Performed under non-reducing conditions.

      Observed band size : 42,44 kDa (why is the actual band size different from the predicted?)


      Exposure time : 1 second

      This image is courtesy of an anonymous Abreview

      See Abreview

    References for EGF protein (Active) (ab9697)

    ab9697 has not yet been referenced specifically in any publications.

    Publishing research using ab9697 ? Please let us know so that we can cite the reference in this datasheet

    Product Wall

    Submit an Abreview for ab9697 Submit a Question for ab9697

    Displaying 1 - 2 of 2 results for Abreviews and Q&A

    10
    Votes: Highest First


    I found three EGF proteins ab9697 (active), ab91371 (active) and ab51082. What’s the difference between active one and not? And what's the difference between two active ones? Which one may I use it to induce the phosphorylation of EGFR in cells...

    Read More

    Thank you for contacting us.
    The two active proteins are essentially the same, and both can be used to induce phosphorylation as they have been tested in functional studies. The only difference would be that ab9697 is supplied as lyophilized powder a...

    Read More

    Western blot Abreview for EGF protein (Active)

     Excellent
    Abreviews
    Application Western blot

    Review text: Human skin fibroblasts (HFF-1) were starved in serum-free medium overnight, and then were incubated 30 min in the presence of either 25 or 50 ng/ml active EGF protein, doses widely used in stem cell culture medium, to test whether EGFR downstream signaling pathway is able to be activated.
    Sample: Human Cell lysate - whole cell (skin fibroblasts)
    Loading amount: 20 µg
    Specification: skin fibroblasts
    Treatment: 25 and 50 ng/ml EGF for 30 min
    Gel Running Conditions: Non-reduced Denaturing (10%)
    Blocking step: Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

    Other product details
    Dilution: 1/1000
    Incubation time: 16 hour(s) and 0 minute(s) · Temperature: 4°C

    Primary antibody (in addition to 'EGF protein (Active)')
    Name: Non-Abcam antibody was used: phospho-p44/42 MAPK (Erk1/2)
    Host species: Rabbit
    Clonality: Polyclonal
    Dilution: 1/1000

    Secondary antibody
    Name: Non-Abcam antibody was used: anti-rabbit 2nd Ab HRP
    Host species: Goat
    Clonality: Polyclonal
    Conjugation: Horse Radish Peroxidase
    Dilution: 1/10000

    Detection
    Detection method: ECL
    Exposure: 1 second(s)
    Bands: Specific: 42/44 kDa

    Additional data
    Additional Notes: Incubation of human fibroblasts with active EGF protein for short time greatly activated EGFR signaling pathway, as the receptor underwent endocytosis and dramatic phosphorylation of p44/42 MAPK.
    abreview image
    Username

    Verified customer

    Submitted Jun 25 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"