EGF protein (Active) (ab9697)
Our Abpromise guarantee covers the use of ab9697 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||WB: Use at an assay dependent dilution.|
|Cellular activation||CellAct: Use at an assay dependent dilution. The ED50, determined by the dose-dependent stimulation of the proliferation of thymidine uptake by BALB/c 3T3 cells, is < 0.1 ng/ml, corresponding to a specific activity of > 1 x 107 units/mg|
|SDS-PAGE||SDS-PAGE: Use at an assay dependent dilution.|
- Beta urogastroneEGFEgf
- EGF_HUMANEpidermal Growth FactorEpidermal Growth FactorHOMG4OTTHUMP00000219721OTTHUMP00000219722Pro epidermal growth factorURGUrogastrone
Contains 9 LDL-receptor class B repeats.
EGF protein (Active) images
Lane 1 :
Lane 2 :
Lane 3 :
Lane 1 : Whole cell lysate of human skin fibroblasts starved overnight in serum-free medium
Lane 2 : Whole cell lysate of human skin fibroblasts starved overnight in serum-free medium and then incubated for 30 min with 25 ng/ml active EGF
Lane 3 : Whole cell lysate of human skin fibroblasts starved overnight in serum-free medium and then incubated for 30 min with 50 ng/ml active EGF
Lysates/proteins at 20 µg per lane.
An HRP-conjugatede Goat anti-rabbit IgG polyclonal at 1/10000 dilution
developed using the ECL technique
Performed under non-reducing conditions.
Observed band size : 42,44 kDa (why is the actual band size different from the predicted?)
Exposure time : 1 second
This image is courtesy of an anonymous Abreview
References for EGF protein (Active) (ab9697)
ab9697 has not yet been referenced specifically in any publications.