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- Primary antibodies
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Product name
EGF protein
See all EGF products (9) ...
Protein description
Recombinant full length protein: corresponding to amino acids 971-1023 of mature Human EGF -(53 amino acids, molecular weight 6.3 kDa).
Expression host
E. coli
Properties
Purity
> 95% by SDS-PAGE
Purification notes
Recombinant EGF was expressed in E.coli and purified by conventional column chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: PBS, pH 7.4
Concentration
Concentration information loading...
Sequence notes
M-NSDSECPLS HDGYCLHDGV CMYIEALDKY ACNCVVGYIG ERCQYRDLKW WELR
Research areas
Stem Cells >> Stem Cell Cytokines >> Recombinant Human
Cancer >> Growth factors >> EGF
Signal Transduction >> Growth Factors/Hormones >> EGF
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Sandwich ELISA - EGF protein (ab51082)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab51082 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
SDS-PAGE: Use at an assay dependent dilution.
Protein info
Function
EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6.
Tissue specificity
Expressed in kidney, salivary gland, cerebrum and prostate.
Involvement in disease
Defects in EGF are the cause of hypomagnesemia type 4 (HOMG4) [MIM:611718]; also known as renal hypomagnesemia normocalciuric. HOMG4 is a disorder characterized by massive renal hypomagnesemia and normal levels of serum calcium and calcium excretion. Clinical features include seizures, mild-to mederate psychomotor retardation, and brisk tendon reflexes.
Sequence similarities
Contains 9 EGF-like domains.
Contains 9 LDL-receptor class B repeats.
Cellular localization
Membrane.
Target information above from: UniProt accessionP01133
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
EGF protein images:
Sandwich ELISA - EGF protein (ab51082)
Standard curve for EGF (Analyte: ab51082); dilution range 1pg/ml to 1µg/ml using Capture Antibody ab18635 at 5µg and Detector Antibody ab9695 at 0.5µg/ml
References for EGF protein (ab51082)
ab51082 has not yet been referenced specifically in any publications.
Publishing research using ab51082? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"