Anti-EGFR antibody [EGFR1] (Biotin) (ab24293)

Overview

  • Product nameAnti-EGFR antibody [EGFR1] (Biotin)See all EGFR primary antibodies ...
  • Description
    Mouse monoclonal [EGFR1] to EGFR (Biotin)
  • ConjugationBiotin
  • Tested applicationsFlow Cyt, IHC-Frmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Human epidermoid carcinoma cells A431.

  • EpitopeRecognizes the extracellular domain of the epidermal growth factor receptor on many cell surfaces.

Properties

Applications

Our Abpromise guarantee covers the use of ab24293 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µg for 106 cells.
IHC-Fr Use at an assay dependent dilution.

Target

  • FunctionReceptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin.
    Isoform 2 may act as an antagonist of EGF action.
  • Tissue specificityUbiquitously expressed. Isoform 2 is also expressed in ovarian cancers.
  • Involvement in diseaseDefects in EGFR are associated with lung cancer (LNCR) [MIM:211980]. LNCR is a common malignancy affecting tissues of the lung. The most common form of lung cancer is non-small cell lung cancer (NSCLC) that can be divided into 3 major histologic subtypes: squamous cell carcinoma, adenocarcinoma, and large cell lung cancer. NSCLC is often diagnosed at an advanced stage and has a poor prognosis.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Phosphorylation at Ser-695 is partial and occurs only if Thr-693 is phosphorylated. Phosphorylation at Thr-678 and Thr-693 by PRKD1 inhibits EGF-induced MAPK8/JNK1 activation. Dephosphorylation by PTPRJ prevents endocytosis and stabilizes the receptor at the plasma membrane. Autophosphorylation at Tyr-1197 is stimulated by methylation at Arg-1199 and enhances interaction with PTPN6. Autophosphorylation at Tyr-1092 and/or Tyr-1110 recruits STAT3.
    Monoubiquitinated and polyubiquitinated upon EGF stimulation; which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting. Polyubiquitin linkage is mainly through 'Lys-63', but linkage through 'Lys-48', 'Lys-11' and 'Lys-29' also occur. Deubiquitinated by OTUD7B, preventing degradation.
    Methylated. Methylation at Arg-1199 by PRMT5 positively stimulates phosphorylation at Tyr-1197.
  • Cellular localizationSecreted and Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus membrane. Nucleus membrane. Endosome. Endosome membrane. In response to EGF, translocated from the cell membrane to the nucleus via Golgi and ER. Endocytosed upon activation by ligand.
  • Information by UniProt
  • Database links
  • Alternative names
    • Avian erythroblastic leukemia viral (v erb b) oncogene homolog antibody
    • Avian erythroblastic leukemia viral (verbb) oncogene homolog antibody
    • Cell growth inhibiting protein 40 antibody
    • Cell proliferation inducing protein 61 antibody
    • EGF R antibody
    • EGFR antibody
    • EGFR antibody
    • EGFR_HUMAN antibody
    • Epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog) antibody
    • Epidermal growth factor receptor (erythroblastic leukemia viral (v erb b) oncogene homolog avian) antibody
    • Epidermal growth factor receptor antibody
    • erbb 1 antibody
    • Erbb antibody
    • Erbb1 antibody
    • ERBB1 antibody
    • Errp antibody
    • HER1 antibody
    • mENA antibody
    • Oncogene ERBB antibody
    • PIG61 antibody
    • Proto-oncogene c-ErbB-1 antibody
    • Receptor tyrosine protein kinase ErbB 1 antibody
    • Receptor tyrosine protein kinase ErbB1 antibody
    • Receptor tyrosine-protein kinase ErbB-1 antibody
    • Urogastrone antibody
    • wa2 antibody
    • Wa5 antibody
    see all

Anti-EGFR antibody [EGFR1] (Biotin) images

  • ab24293, at a 1/45 dilution, staining EGFR in human glioblastoma U87MGwtEGFR cells by Flow Cytometry analysis. The image shows cells with (blue) and without (red) EGFR1-biotin primary antibody. In lieu of a secondary antibody, a streptavidin-R-phycoerythrin conjugate was used as a secondary reagent at a 1/83 dilution.

    See Abreview

References for Anti-EGFR antibody [EGFR1] (Biotin) (ab24293)

This product has been referenced in:
  • Burel-Vandenbos F  et al. Cells with intense EGFR staining and a high nuclear to cytoplasmic ratio are specific for infiltrative glioma: a useful marker in neuropathological practice. Neuro Oncol 15:1278-88 (2013). Read more (PubMed: 23935154) »
  • Yonesaka K  et al. Activation of ERBB2 signaling causes resistance to the EGFR-directed therapeutic antibody cetuximab. Sci Transl Med 3:99ra86 (2011). Read more (PubMed: 21900593) »

See all 4 Publications for this product

Product Wall

Thank you for your inquiry.

For ab24293, the laboratory has stated that the biotinylation procedure is proprietary. The only information they will release is that the procedure is designed to assure the degree of biotinylation is no greater t...

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I heard back from the lab that unfortunately this has not been tested so the information is not available.
I'm sorry that we couldn't be of more help in this regard, but please let me know if you have any addi...

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The antibody ab30 will be covered under our Abpromise guarantee for use in ICC on human cells. If for any reason you are unsatisfied with the results, we will be happy to refund or replace it for you as long as you c...

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Thank you for your enquiry and your interest in our products.

I can confirm that neither of these antibodies have been characterized for inhibition studies. ab24284 is suitable for IHC-P only; whilst ab24293 has been tested for Flow cytometry...

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Thank you for your call today and for our discussion of these EGFR antibodies.

Please let me know if you have any further questions or would like any assistance choosing reagents for your studies.

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Application Flow Cytometry
Sample Human Cell (U87MG.wtEGFR)
Specification U87MG.wtEGFR
Preparation Cell harvesting/tissue preparation method: cell line
Sample buffer: PBS + 1 g/L BSA
Fixation not fixed
Permeabilization No
Gating Strategy forward scatter and side scatter
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Submitted Aug 27 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"