Anti-EGFR antibody [EGFR1] (Biotin) (ab24293)

Overview

  • Product nameAnti-EGFR antibody [EGFR1] (Biotin)
    See all EGFR primary antibodies
  • Description
    Mouse monoclonal [EGFR1] to EGFR (Biotin)
  • ConjugationBiotin
  • Tested applicationsFlow Cyt, IHC-Frmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Human epidermoid carcinoma cells A431.

  • EpitopeRecognizes the extracellular domain of the epidermal growth factor receptor on many cell surfaces.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab24293 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µg for 106 cells.

ab18418 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

IHC-Fr Use at an assay dependent concentration.

Target

  • Information by UniProt
  • Database links
  • Alternative names
    • Avian erythroblastic leukemia viral (v erb b) oncogene homolog antibody
    • Cell growth inhibiting protein 40 antibody
    • Cell proliferation inducing protein 61 antibody
    • EGF R antibody
    • EGFR antibody
    • EGFR_HUMAN antibody
    • Epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog) antibody
    • Epidermal growth factor receptor (erythroblastic leukemia viral (v erb b) oncogene homolog avian) antibody
    • Epidermal growth factor receptor antibody
    • erb-b2 receptor tyrosine kinase 1 antibody
    • ERBB antibody
    • ERBB1 antibody
    • Errp antibody
    • HER1 antibody
    • mENA antibody
    • NISBD2 antibody
    • Oncogen ERBB antibody
    • PIG61 antibody
    • Proto-oncogene c-ErbB-1 antibody
    • Receptor tyrosine protein kinase ErbB 1 antibody
    • Receptor tyrosine-protein kinase ErbB-1 antibody
    • SA7 antibody
    • Species antigen 7 antibody
    • Urogastrone antibody
    • v-erb-b Avian erythroblastic leukemia viral oncogen homolog antibody
    • wa2 antibody
    • Wa5 antibody
    see all

Anti-EGFR antibody [EGFR1] (Biotin) images

  • ab24293, at a 1/45 dilution, staining EGFR in human glioblastoma U87MGwtEGFR cells by Flow Cytometry analysis. The image shows cells with (blue) and without (red) EGFR1-biotin primary antibody. In lieu of a secondary antibody, a streptavidin-R-phycoerythrin conjugate was used as a secondary reagent at a 1/83 dilution.

    See Abreview

References for Anti-EGFR antibody [EGFR1] (Biotin) (ab24293)

This product has been referenced in:
  • Burel-Vandenbos F  et al. Cells with intense EGFR staining and a high nuclear to cytoplasmic ratio are specific for infiltrative glioma: a useful marker in neuropathological practice. Neuro Oncol 15:1278-88 (2013). Read more (PubMed: 23935154) »
  • Yonesaka K  et al. Activation of ERBB2 signaling causes resistance to the EGFR-directed therapeutic antibody cetuximab. Sci Transl Med 3:99ra86 (2011). Read more (PubMed: 21900593) »

See all 4 Publications for this product

Product Wall

Thank you for your inquiry.

For ab24293, the laboratory has stated that the biotinylation procedure is proprietary. The only information they will release is that the procedure is designed to assure the degree of biotinylation is no greater t...

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Thank you for your inquiry.

I heard back from the lab that unfortunately this has not been tested so the information is not available.
I'm sorry that we couldn't be of more help in this regard, but please let me know if you have any addi...

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Thank you for your reply.

The antibody ab30 will be covered under our Abpromise guarantee for use in ICC on human cells. If for any reason you are unsatisfied with the results, we will be happy to refund or replace it for you as long as you c...

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Thank you for your enquiry and your interest in our products.

I can confirm that neither of these antibodies have been characterized for inhibition studies. ab24284 is suitable for IHC-P only; whilst ab24293 has been tested for Flow cytometry...

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Thank you for your call today and for our discussion of these EGFR antibodies.

Please let me know if you have any further questions or would like any assistance choosing reagents for your studies.

I hope you have a great day!

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (U87MG.wtEGFR)
Specification U87MG.wtEGFR
Preparation Cell harvesting/tissue preparation method: cell line
Sample buffer: PBS + 1 g/L BSA
Fixation not fixed
Permeabilization No
Gating Strategy forward scatter and side scatter
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Verified customer

Submitted Aug 27 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"