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Anti-EGFR antibody [EGFR1] (ab30)

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Overview

Product name

Anti-EGFR antibody [EGFR1]
See all EGFR products (87) ...

Description

Mouse monoclonal [EGFR1] to EGFR

Specificity

This antibody does not react with erbB-2, erbB-3 and erbB-4.

Tested applications

IHC-Fr, ICC/IF, IP, Flow Cytmore details

Cross reactivity

Reacts with

Mouse, Horse, Human

Immunogen

Human epidermoid carcinoma line A431; epitope mapped between aa 6-273 of human EGFR.

Epitope

Extracellular.

General notes

Recognises the external EGF-binding domain of the EGFR transmembrane glycoprotein. No effect on tyrosine kinase activity of EGFR.

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: None
Constituents: PBS

Concentration

Concentration information loading...

Purity

Protein A purified

Clonality

Monoclonal

Clone number

EGFR1

Myeloma

P3-NS1/1-Ag4-1

Isotype

IgG2b

Light chain type

kappa

Research areas

Cancer >> Tumor biomarkers >> Receptors
Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Growth factor receptors
Cancer >> Signal transduction >> Protein phosphorylation >> Tyrosine kinases >> Receptor tyrosine kinases
Signal Transduction >> Growth Factors/Hormones >> EGF
Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Receptor Tyrosine Kinases

  • Immunocytochemistry/ Immunofluorescence-EGFR antibody [EGFR1](ab30)Immunocytochemistry/ Immunofluorescence-EGFR antibody [EGFR1](ab30) image (enlarge)

  • Flow Cytometry - EGFR antibody [EGFR1] (ab30)Flow Cytometry - EGFR antibody [EGFR1] (ab30) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab30 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

Is unsuitable for ,ELISA or WB.

Target

Function

Receptor for EGF, but also for other members of the EGF family, as TGF-alpha, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. Is involved in the control of cell growth and differentiation. Phosphorylates MUC1 in breast cancer cells and increases the interaction of MUC1 with SRC and CTNNB1/beta-catenin.
Isoform 2 may act as an antagonist of EGF action.

Tissue specificity

Ubiquitously expressed. Isoform 2 is also expressed in ovarian cancers.

Involvement in disease

Defects in EGFR are associated with lung cancer (LNCR) [MIM:211980].

Sequence similarities

Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
Contains 1 protein kinase domain.

Post-translational
modifications

Phosphorylation of Ser-695 is partial and occurs only if Thr-693 is phosphorylated.
Monoubiquitinated and polyubiquitinated upon EGF stimulation; which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting. Polyubiquitin linkage is mainly through 'Lys-63', but linkage through 'Lys-48', 'Lys-11' and 'Lys-29' also occur.

Cellular localization

Secreted and Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus membrane. Nucleus membrane. Endosome. In response to EGF, translocated from the cell membrane to the nucleus via Golgi and ER. Co-localizes with TNK2 on the endosomes.

Target information above from: UniProt accessionP00533 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • EGFR antibody
  • Avian erythroblastic leukemia viral (v erb b) oncogene homolog antibody
  • Avian erythroblastic leukemia viral (verbb) oncogene homolog antibody
  • Cell growth inhibiting protein 40 antibody
  • Cell proliferation inducing protein 61 antibody
  • EGF R antibody
  • EGFR antibody
  • EGFR_HUMAN antibody
  • Epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog) antibody
  • Epidermal growth factor receptor (erythroblastic leukemia viral (v erb b) oncogene homolog avian) antibody
  • Epidermal growth factor receptor antibody
  • erbb 1 antibody
  • Erbb antibody
  • Erbb1 antibody
  • ERBB1 antibody
  • HER1 antibody
  • mENA antibody
  • Oncogene ERBB antibody
  • PIG61 antibody
  • Proto-oncogene c-ErbB-1 antibody
  • Receptor tyrosine protein kinase ErbB 1 antibody
  • Receptor tyrosine protein kinase ErbB1 antibody
  • Receptor tyrosine-protein kinase ErbB-1 antibody
  • Urogastrone antibody
  • wa2 antibody
  • Wa5 antibody
see all

Database links

see all

Anti-EGFR antibody [EGFR1] images:

  Immunocytochemistry/ Immunofluorescence-EGFR antibody [EGFR1](ab30)

Immunocytochemistry/ Immunofluorescence-EGFR antibody [EGFR1](ab30)

ICC/IF image of ab30 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab30, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  Flow Cytometry - EGFR antibody [EGFR1] (ab30)

Flow Cytometry - EGFR antibody [EGFR1] (ab30)

Overlay histogram showing HEK293 cells stained with ab30 (red line). The cells were incubated with the antibody (ab30, 1µg/1x106 cells) for 30 min at 4°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/200 dilution for 30 min at 4°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Please note that 4% paraformaldehyde (10 min) or methanol (5 min) fixation prior to antibody staining significantly decreased signal.

References for Anti-EGFR antibody [EGFR1] (ab30)

This product has been referenced in:

  • Friedman Met al. Engineering and characterization of a bispecific HER2 x EGFR-binding affibody molecule. Biotechnol Appl Biochem 54:121-31 (2009). Flow Cyt; Human.Read more (PubMed: 19492986) »
  • Jensen KB & Watt FM Single-cell expression profiling of human epidermal stem and transit-amplifying cells: Lrig1 is a regulator of stem cell quiescence. Proc Natl Acad Sci U S A 103:11958-63 (2006).Read more (PubMed: 16877544) »

See all 2 publications for this product

Publishing research using ab30? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"