Overview

  • Product nameAnti-EGFR antibody [EGFR1]
    See all EGFR primary antibodies
  • Description
    Mouse monoclonal [EGFR1] to EGFR
  • SpecificityThis antibody does not react with erbB-2, erbB-3 and erbB-4.
  • Tested applicationsIHC-Fr, ICC/IF, IP, Flow Cyt, IHC - Wholemountmore details
  • Species reactivity
    Reacts with: Mouse, Horse, Human
  • Immunogen

    Tissue, cells or virus corresponding to Human EGFR (extracellular). Human epidermoid carcinoma line A431; epitope mapped between aa 6-273 of human EGFR.

  • EpitopeExtracellular.
  • General notes

    Recognises the external EGF-binding domain of the EGFR transmembrane glycoprotein. No effect on tyrosine kinase activity of EGFR.

Properties

Applications

Our Abpromise guarantee covers the use of ab30 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
ICC/IF Use a concentration of 1 µg/ml.
IP Use at an assay dependent concentration.
Flow Cyt Use 1µg for 106 cells.

(unsuitable for fixed cells)

IHC - Wholemount Use at an assay dependent concentration.
  • Application notesIs unsuitable for ELISA or WB.
  • Target

    • Information by UniProt
    • Database links
    • Alternative names
      • Avian erythroblastic leukemia viral (v erb b) oncogene homolog antibody
      • Cell growth inhibiting protein 40 antibody
      • Cell proliferation inducing protein 61 antibody
      • EGF R antibody
      • EGFR antibody
      • EGFR_HUMAN antibody
      • Epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog) antibody
      • Epidermal growth factor receptor (erythroblastic leukemia viral (v erb b) oncogene homolog avian) antibody
      • Epidermal growth factor receptor antibody
      • erb-b2 receptor tyrosine kinase 1 antibody
      • ERBB antibody
      • ERBB1 antibody
      • Errp antibody
      • HER1 antibody
      • mENA antibody
      • NISBD2 antibody
      • Oncogen ERBB antibody
      • PIG61 antibody
      • Proto-oncogene c-ErbB-1 antibody
      • Receptor tyrosine protein kinase ErbB 1 antibody
      • Receptor tyrosine-protein kinase ErbB-1 antibody
      • SA7 antibody
      • Species antigen 7 antibody
      • Urogastrone antibody
      • v-erb-b Avian erythroblastic leukemia viral oncogen homolog antibody
      • wa2 antibody
      • Wa5 antibody
      see all

    Anti-EGFR antibody [EGFR1] images

    • ICC/IF image of ab30 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab30, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • Overlay histogram showing HEK293 cells stained with ab30 (red line). The cells were incubated with the antibody (ab30, 1µg/1x106 cells) for 30 min at 4°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/200 dilution for 30 min at 4°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
      Please note that 4% paraformaldehyde (10 min) or methanol (5 min) fixation prior to antibody staining significantly decreased signal.

    References for Anti-EGFR antibody [EGFR1] (ab30)

    This product has been referenced in:
    • Leung SJ  et al. In vivo molecular mapping of the tumor microenvironment in an azoxymethane-treated mouse model of colon carcinogenesis. Lasers Surg Med 47:40-9 (2015). Read more (PubMed: 25487746) »
    • Kim J  et al. microRNA-148a is a prognostic oncomiR that targets MIG6 and BIM to regulate EGFR and apoptosis in glioblastoma. Cancer Res 74:1541-53 (2014). Read more (PubMed: 24425048) »

    See all 6 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application IHC - Wholemount
    Sample Human Tissue (Brain)
    Specification Brain
    Username

    Abcam user community

    Verified customer

    Submitted Feb 22 2013

    Thank you for contacting us.

    Rabbit monoclonals typically have 10-100x the affinity of mouse monoclonals, as such it is often the case that the concentration of a rabbit monoclonal will be lower than a mouse monoclonal. Ab52894 does not ha...

    Read More

    DISCOUNT CODE: ***
    Expiration date: November 13th, 2012

    I am very pleased to hear you would like to accept our offer and test ab30 in IHC-PFA. This code will give you: 1 free primary antibody before the expiration date. To redeem this of...

    Read More

    Vielen Dank für Ihren Anruf und für Ihr Interesse an unseren Produkten.

    Hier ist die Liste der monoklonalen Antikörper gegen EGFR, welche wir am Telefon diskutiert haben:

    ab30, unconjugated, tested in flow cytometry a...

    Read More

    Thank you for your reply.

    The antibody ab30 will be covered under our Abpromise guarantee for use in ICC on human cells. If for any reason you are unsatisfied with the results, we will be happy to refund or replace it for you as long as you c...

    Read More

    Thank you for your call today and for our discussion of these EGFR antibodies.

    Please let me know if you have any further questions or would like any assistance choosing reagents for your studies.

    I hope you have a great day!

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunoprecipitation
    Sample Mouse Cell lysate - whole cell (sperm)
    Total protein in input 1e+008 cells
    Specification sperm
    Immuno-precipitation step Protein A/G
    Username

    Dr. Yael Jaldety

    Verified customer

    Submitted Jun 24 2011

    I'm sorry to hear you are still having problems with the replacement of ab30, this must be very fustrating. I understand that my colleague Danielle has raised the possibility that "this antibody does not recognise recombinant EGFR. The epitope has bee...

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    The protocol that your customer is using looks fine so I would be happy to send you a replacement vial just in case there is a problem with the original vial that they received. However, it is possible that this antibody does not recognise recombinant ...

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    Thank you again for your patience. Regarding ab30, EGFR1 recognises the whole receptor protein rather than a truncated version. For ab5636, the synthetic peptide immunogen to produce the antibody is approximately 10 amino acid residues around EGFR [pY8...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"