Anti-EGFR antibody [EP38Y] (ab52894)
- Product nameAnti-EGFR antibody [EP38Y]See all EGFR primary antibodies ...
- DescriptionRabbit monoclonal [EP38Y] to EGFR
- SpecificityThis antibody detects both EGFR phosphorylated on Tyrosine 1068 of the mature human isoform 1 (corresponding to Y1092 from the precursor form P00533-1/p170), and also unphosphorylated EGFR.
- Tested applicationsWB, IP, IHC-P, ICC/IF more details
- Species reactivityReacts with: Mouse, Rat, Human
Synthetic phospho-peptide corresponding to residues surrounding Tyr1068 of human EGFR.
- Positive controlHeLa cell lysate or human lymph node metastasis tissue.
- General notesProduced under U.S. Patent No. 5,675,063.
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Dissociation constant (Kd) Kd = 1.90 x 10-11 M
- Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
- PurityTissue culture supernatant
- Clonality Monoclonal
- Clone numberEP38Y
Our Abpromise guarantee covers the use of ab52894 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||WB: 1/100000. Detects a band of approximately 175 kDa (predicted molecular weight: 134 kDa).|
|IHC-P||IHC-P: Use at an assay dependent dilution.|
|ICC/IF||ICC/IF: 1/250 - 1/500.|
- FunctionReceptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin.
Isoform 2 may act as an antagonist of EGF action.
- Tissue specificityUbiquitously expressed. Isoform 2 is also expressed in ovarian cancers.
- Involvement in diseaseDefects in EGFR are associated with lung cancer (LNCR) [MIM:211980]. LNCR is a common malignancy affecting tissues of the lung. The most common form of lung cancer is non-small cell lung cancer (NSCLC) that can be divided into 3 major histologic subtypes: squamous cell carcinoma, adenocarcinoma, and large cell lung cancer. NSCLC is often diagnosed at an advanced stage and has a poor prognosis.
- Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
Contains 1 protein kinase domain.
modificationsPhosphorylation at Ser-695 is partial and occurs only if Thr-693 is phosphorylated. Phosphorylation at Thr-678 and Thr-693 by PRKD1 inhibits EGF-induced MAPK8/JNK1 activation. Dephosphorylation by PTPRJ prevents endocytosis and stabilizes the receptor at the plasma membrane. Autophosphorylation at Tyr-1197 is stimulated by methylation at Arg-1199 and enhances interaction with PTPN6. Autophosphorylation at Tyr-1092 and/or Tyr-1110 recruits STAT3.
Monoubiquitinated and polyubiquitinated upon EGF stimulation; which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting. Polyubiquitin linkage is mainly through 'Lys-63', but linkage through 'Lys-48', 'Lys-11' and 'Lys-29' also occur. Deubiquitinated by OTUD7B, preventing degradation.
Methylated. Methylation at Arg-1199 by PRMT5 positively stimulates phosphorylation at Tyr-1197.
- Cellular localizationSecreted and Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus membrane. Nucleus membrane. Endosome. Endosome membrane. In response to EGF, translocated from the cell membrane to the nucleus via Golgi and ER. Endocytosed upon activation by ligand.
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Anti-EGFR antibody [EP38Y] images
Anti-EGFR antibody [EP38Y] (ab52894) at 1/100000 dilution + HeLa cell lysate at 10 µg
Goat anti-Rabbit HRP labeled. at 1/2000 dilution
Predicted band size : 134 kDa
Observed band size : 175 kDa (why is the actual band size different from the predicted?)
Ab52894 (1:100) staining human EGFR in human lymph node metastasis tissue by immunohistochemistry using paraffin embedded tissue.
ab52894 showing positive staining in Cervical carcinoma tissue.
ab52894 showing positive staining in Endometrial carcinoma tissue.
ab52894 showing positive staining in Glioma tissue.
ab52894 showing positive staining in Normal tonsil squamous cells tissue.
ab52894 showing positive staining in Renal cell carcinoma tissue.
Equilibrium disassociation constant (KD)
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References for Anti-EGFR antibody [EP38Y] (ab52894)
This product has been referenced in:
- Modena P et al. Predictors of outcome in an AIEOP series of childhood ependymomas: a multifactorial analysis. Neuro Oncol 14:1346-56 (2012). IHC ; Human . Read more (PubMed: 23076205) »
- Raina D et al. Dependence on the MUC1-C oncoprotein in non-small cell lung cancer cells. Mol Cancer Ther 10:806-16 (2011). IP ; Human . Read more (PubMed: 21421804) »