Anti-EGFR antibody [EP38Y] (ab52894)

Overview

• Product nameAnti-EGFR antibody [EP38Y]See all EGFR primary antibodies ...
• Description
  Rabbit monoclonal [EP38Y] to EGFR
• SpecificityThis antibody detects both EGFR phosphorylated on Tyrosine 1068 of the mature human isoform 1 (corresponding to Y1092 from the precursor form P00533-1/p170), and also unphosphorylated EGFR.
• Tested applicationsWB, IP, IHC-P, ICC/IF more details
• Species reactivity
  Reacts with: Mouse, Rat, Human
  
• Immunogen

  Synthetic phospho-peptide corresponding to residues surrounding Tyr1068 of human EGFR.

• Positive controlHeLa cell lysate or human lymph node metastasis tissue.
• General notesProduced under U.S. Patent No. 5,675,063.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Dissociation constant (Kd) Kd = 1.90 x 10^-11 M
• Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
• PurityTissue culture supernatant
• Clonality Monoclonal
• Clone numberEP38Y
• IsotypeIgG
• Research Areas
  • Cancer
  • Oncoproteins/suppressors
  • Oncoproteins
  • Growth factor receptors

  • Cancer
  • Tumor biomarkers
  • Receptors

  • Cancer
  • Signal transduction
  • Protein phosphorylation
  • Tyrosine kinases
  • Receptor tyrosine kinases

  • Signal Transduction
  • Growth Factors/Hormones
  • EGF

  • Signal Transduction
  • Protein Phosphorylation
  • Tyrosine Kinases
  • Receptor Tyrosine Kinases

Applications

Target

• FunctionReceptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin.
  Isoform 2 may act as an antagonist of EGF action.
• Tissue specificityUbiquitously expressed. Isoform 2 is also expressed in ovarian cancers.
• Involvement in diseaseDefects in EGFR are associated with lung cancer (LNCR) [MIM:211980]. LNCR is a common malignancy affecting tissues of the lung. The most common form of lung cancer is non-small cell lung cancer (NSCLC) that can be divided into 3 major histologic subtypes: squamous cell carcinoma, adenocarcinoma, and large cell lung cancer. NSCLC is often diagnosed at an advanced stage and has a poor prognosis.
• Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
  Contains 1 protein kinase domain.
• Post-translational
  modificationsPhosphorylation at Ser-695 is partial and occurs only if Thr-693 is phosphorylated. Phosphorylation at Thr-678 and Thr-693 by PRKD1 inhibits EGF-induced MAPK8/JNK1 activation. Dephosphorylation by PTPRJ prevents endocytosis and stabilizes the receptor at the plasma membrane. Autophosphorylation at Tyr-1197 is stimulated by methylation at Arg-1199 and enhances interaction with PTPN6. Autophosphorylation at Tyr-1092 and/or Tyr-1110 recruits STAT3.
  Monoubiquitinated and polyubiquitinated upon EGF stimulation; which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting. Polyubiquitin linkage is mainly through 'Lys-63', but linkage through 'Lys-48', 'Lys-11' and 'Lys-29' also occur. Deubiquitinated by OTUD7B, preventing degradation.
  Methylated. Methylation at Arg-1199 by PRMT5 positively stimulates phosphorylation at Tyr-1197.
• Cellular localizationSecreted and Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus membrane. Nucleus membrane. Endosome. Endosome membrane. In response to EGF, translocated from the cell membrane to the nucleus via Golgi and ER. Endocytosed upon activation by ligand.

Target information above from: UniProt accession P00533 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 1956 Human
  • Entrez Gene: 13649 Mouse
  • Entrez Gene: 24329 Rat
  • Omim: 131550 Human
  • SwissProt: P00533 Human
  • SwissProt: Q01279 Mouse
  • Unigene: 488293 Human
  • Unigene: 420648 Mouse

  • Unigene: 439882 Mouse
  • Unigene: 8534 Mouse
  • Unigene: 37227 Rat

  see all

• Alternative names
  Avian erythroblastic leukemia viral (v erb b) oncogene homolog antibodyCell growth inhibiting protein 40 antibodyCell proliferation inducing protein 61 antibody

  EGF R antibodyEGFR antibodyEGFR_HUMAN antibodyEpidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog) antibodyEpidermal growth factor receptor (erythroblastic leukemia viral (v erb b) oncogene homolog avian) antibodyEpidermal growth factor receptor antibodyerbb 1 antibodyErbb antibodyErbb1 antibodyERBB1 antibodyErrp antibodyHER1 antibodymENA antibodyOncogene ERBB antibodyPIG61 antibodyProto-oncogene c-ErbB-1 antibodyReceptor tyrosine protein kinase ErbB 1 antibodyReceptor tyrosine-protein kinase ErbB-1 antibodyUrogastrone antibodywa2 antibodyWa5 antibody

  see all

Anti-EGFR antibody [EP38Y] images

• 

  Western blot - EGFR antibody [EP38Y] (ab52894)
  Anti-EGFR antibody [EP38Y] (ab52894) at 1/100000 dilution + HeLa cell lysate at 10 µg
  
  Secondary
  Goat anti-Rabbit HRP labeled. at 1/2000 dilution
  
  Predicted band size : 134 kDa
  Observed band size : 175 kDa (why is the actual band size different from the predicted?)
  
• 

  Immunohistochemistry (Paraffin-embedded sections) - EGFR antibody [EP38Y] (ab52894)
  Ab52894 (1:100) staining human EGFR in human lymph node metastasis tissue by immunohistochemistry using paraffin embedded tissue.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-EGFR antibody [EP38Y](ab52894)
  ab52894 showing positive staining in Cervical carcinoma tissue.
  
  
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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-EGFR antibody [EP38Y](ab52894)
  ab52894 showing positive staining in Endometrial carcinoma tissue.
  
  
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-EGFR antibody [EP38Y](ab52894)
  ab52894 showing positive staining in Glioma tissue.
  
  
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-EGFR antibody [EP38Y](ab52894)
  ab52894 showing positive staining in Normal tonsil squamous cells tissue.
  
  
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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-EGFR antibody [EP38Y](ab52894)
  ab52894 showing positive staining in Renal cell carcinoma tissue.
  
  
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  Other-Anti-EGFR antibody [EP38Y](ab52894)
  Equilibrium disassociation constant (K_D)
  Learn more about K_D
  
  Click here to learn more about K_D