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Dear Technical support,
I am Suping working in UCSD and is looking for EGFR antibody for IHC-P. I did find some EGFR antibodies (e.g.ab52894). However, it does not indicate if need unmasking procedure and what method for this step. Would you please recommend EGFR monoclonal antibodies and related infos for me? Thanks.
Asked on Jan 10 2013
Hello! We do recommend you use an antigen retrieval step, heat mediated such as citrate pH6. The antibody you suggest, ab52894 is a good one. Another EGFR antibody with good feedback is ab9482. We do guarantee our products to work as specified on the datasheet for 6 months of purchase. Please let me know if you have further questions.
Abcam Scientific Support
Answered on Jan 10 2013