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Thank you for all the advice and the help you gave me
Please I was reading the protocol of the IHC kit I bought and I would like to have more information about the 3rd step in the staining protocol when you speak about perfoming an appropriate "pretreatment "if required. Wash 3 times in buffer.
Which kind of pretreatment? I want to have more details please I will be so thankful
Asked on Jul 14 2014
The pretreatment note in the protocol of the IHC kit refers to the antigen retrieval step that is required for most antibody to achieve a successful IHC-P staining.
For both of these antibodies (ab14440 and ab52894) I can recommend to use Sodium Citrate Buffer pH 6.0.
Please find more information on antigen retrieval here:
We offer 10 x Sodium Citrate Buffer pH 6.0 for this purpose:
ab64214 (10x Citrate Buffer pH 6.0) http://www.abcam.com/index.html?datasheet=64214 http://www.abcam.com/index.html?datasheet=64214.
Please note that this step often requires intense optimisation and I can recommend to try a time curve to find the best antigen protocol for your samples and antibodies.
I wish you good luck with your research.
Anja Hoffmann Abcam Scientific Support
Answered on Jul 14 2014