Western blot abreview for Anti-EGFR antibody [EP38Y]

Good
Abreviews
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (4-15%)
Sample
Human Cell lysate - whole cell (non small cell lung cancer)
Specification
non small cell lung cancer
Treatment
siRNA treated
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA
Dilution
1/1000

Secondary antibody

Name
Non-Abcam antibody was used: GE Healthcare Anti Rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000

Detection

Detection method
ECL
Bands
Specific: ~165 kDa
Exposure
40 second(s)

Additional data

Additional Notes
Lane 1: H820 siCtl Lane 2: H820 siEGFR#1 Lane 3:: H820 siEGFR#2
Username

Abcam user community

Verified customer

Submitted Oct 07 2014

Sign up