Western blot abreview for Anti-EGFR antibody [EP38Y]

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (4-15%)
Sample Human Cell lysate - whole cell (non small cell lung cancer)
Specification non small cell lung cancer
Treatment siRNA treated
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time 16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA
Dilution 1/1000

Secondary antibody

Name Non-Abcam antibody was used: GE Healthcare Anti Rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution 1/5000


Detection method ECL
Bands Specific: ~165 kDa
Exposure 40 second(s)

Additional data

Additional Notes Lane 1: H820 siCtl Lane 2: H820 siEGFR#1 Lane 3:: H820 siEGFR#2

Abcam user community

Verified customer

Submitted Oct 07 2014