Immunocytochemistry/ Immunofluorescence abreview for Anti-EGFR antibody [EP38Y]

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Permeabilization Yes - 0.5% Triton X100 in PBS
Fixative Paraformaldehyde

Other product details

Incubation time 1 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: PBS
Dilution 1/200

Secondary antibody

Dilution 1/200

Additional data

Additional Notes Asynchronous cells were paraformaldehyde-fixed, permeablized, immuno-fluorescently labeled and counterstained with DAPI. This antibody produced the expected membrane and cytoplasmic staining pattern under these conditions. MeOH-fixed samples were also evaluate and also produced the expected staining pattern.

Abcam user community

Verified customer

Submitted Mar 25 2014