Immunocytochemistry/ Immunofluorescence abreview for Anti-EGFR antibody [EP38Y]

Excellent
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Permeabilization
Yes - 0.5% Triton X100 in PBS
Fixative
Paraformaldehyde

Other product details

Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: PBS
Dilution
1/200

Secondary antibody

Dilution
1/200

Additional data

Additional Notes
Asynchronous cells were paraformaldehyde-fixed, permeablized, immuno-fluorescently labeled and counterstained with DAPI. This antibody produced the expected membrane and cytoplasmic staining pattern under these conditions. MeOH-fixed samples were also evaluate and also produced the expected staining pattern.
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Submitted Mar 25 2014

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