Western blot abreview for Anti-EGFR antibody [EP38Y]

Application Western blot
Sample Mouse Cell lysate - other (macrophage)
Gel Running Conditions Reduced Denaturing
Loading amount 80 µg
Specification macrophage
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time 14 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS-T with 0.25% BSA
Dilution 1/500

Secondary antibody

Dilution 1/5000
Name Abcam antibody:Goat Anti-Rabbit IgG H&L (HRP)


Detection method ECL
Exposure 1 minute(s) and 0 second(s)
Bands Specific: ~170 kDa

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Submitted Nov 06 2015