Immunocytochemistry/ Immunofluorescence abreview for Anti-EGFR antibody [EP38Y]

Average
Abreviews
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (D3 mouse embryonic stem cells)
Permeabilization Yes - Triton 0.1%
Specification D3 mouse embryonic stem cells
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 1%
Fixative Paraformaldehyde

Other product details

Incubation time 2 hour(s) and 0 minute(s) · Temperature: 15°C · Diluent: blocking solution with serum
Dilution 1/100

Secondary antibody

Dilution 1/500
Name Non-Abcam antibody was used: Goat anti-Rabbit IgG (H+L) Secondary Antibody, Ale
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Username

Miss. Fabiana Luise

Verified customer

Submitted Oct 22 2015