Western blot abreview for Anti-EGFR antibody [EP38Y]

Good
Abreviews
Application Western blot
Sample Human Cell lysate - whole cell (Human Renal Epithelial Cells)
Gel Running Conditions Non-reduced Non-Denaturing (Native) (8%)
Loading amount 60 µg
Treatment 20ng EGF for 30min
Specification Human Renal Epithelial Cells
Blocking step BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Other product details

Incubation time 4 hour(s) and 0 minute(s) · Temperature: 25°C
Dilution 1/1000

Secondary antibody

Dilution 1/5000
Name Non-Abcam antibody was used: anti-rabbit
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method ecl
Exposure 10 minute(s) and 0 second(s)
Bands Specific: 175 kDa
Username

Dr. 腾飞 白

Verified customer

Submitted Jan 06 2016