Western blot abreview for Anti-EGFR antibody [EP38Y]

Good
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (Human Renal Epithelial Cells)
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (8%)
Loading amount
60 µg
Treatment
20ng EGF for 30min
Specification
Human Renal Epithelial Cells
Blocking step
BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Other product details

Incubation time
4 hour(s) and 0 minute(s) · Temperature: 25°C
Dilution
1/1000

Secondary antibody

Dilution
1/5000
Name
Non-Abcam antibody was used: anti-rabbit
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ecl
Exposure
10 minute(s) and 0 second(s)
Bands
Specific: 175 kDa
Username

Dr. 腾飞 白

Verified customer

Submitted Jan 06 2016

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