Western blot abreview for Anti-EGFR antibody [EP38Y]

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Abreviews
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Application Western blot
Sample Mouse Tissue lysate - whole (Kidney)
Gel Running Conditions Reduced Denaturing (4-12%)
Loading amount 50 µg
Treatment Unilateral Ureter Obstruction Surgery
Specification Kidney
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time 16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% Milk
Dilution 1/1000

Secondary antibody

Dilution 1/3000
Name Non-Abcam antibody was used: Cell Signaling Anti-Rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method ECL+
Exposure 2 minute(s) and 0 second(s)
Bands Specific: 170 kDa
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Submitted Apr 05 2017