Anti-EGFR antibody [ICR10] (FITC) (ab11400)


  • Product nameAnti-EGFR antibody [ICR10] (FITC)
    See all EGFR primary antibodies
  • Description
    Rat monoclonal [ICR10] to EGFR (FITC)
  • ConjugationFITC. Ex: 493nm, Em: 528nm
  • Tested applicationsFlow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    The extracellular domain of human EGFR from head and neck carcinoma.

  • EpitopeBinds Epitope B with an affinity of 6.7 x 10-9 M.
  • General notesThis product should be stored undiluted. Storage in frost-free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.



Our Abpromise guarantee covers the use of ab11400 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10. Use 10ul of the suggested working dilution to label 106cells in 100ul.

ab18446-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.


  • FunctionReceptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin.
    Isoform 2 may act as an antagonist of EGF action.
  • Tissue specificityUbiquitously expressed. Isoform 2 is also expressed in ovarian cancers.
  • Involvement in diseaseLung cancer
    Inflammatory skin and bowel disease, neonatal, 2
  • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    Phosphorylation at Ser-695 is partial and occurs only if Thr-693 is phosphorylated. Phosphorylation at Thr-678 and Thr-693 by PRKD1 inhibits EGF-induced MAPK8/JNK1 activation. Dephosphorylation by PTPRJ prevents endocytosis and stabilizes the receptor at the plasma membrane. Autophosphorylation at Tyr-1197 is stimulated by methylation at Arg-1199 and enhances interaction with PTPN6. Autophosphorylation at Tyr-1092 and/or Tyr-1110 recruits STAT3. Dephosphorylated by PTPN1 and PTPN2.
    Monoubiquitinated and polyubiquitinated upon EGF stimulation; which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting. Polyubiquitin linkage is mainly through 'Lys-63', but linkage through 'Lys-48', 'Lys-11' and 'Lys-29' also occurs. Deubiquitination by OTUD7B prevents degradation. Ubiquitinated by RNF115 and RNF126.
    Methylated. Methylation at Arg-1199 by PRMT5 stimulates phosphorylation at Tyr-1197.
  • Cellular localizationSecreted and Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus membrane. Nucleus membrane. Endosome. Endosome membrane. Nucleus. In response to EGF, translocated from the cell membrane to the nucleus via Golgi and ER. Endocytosed upon activation by ligand. Colocalized with GPER1 in the nucleus of estrogen agonist-induced cancer-associated fibroblasts (CAF).
  • Information by UniProt
  • Database links
  • Alternative names
    • Avian erythroblastic leukemia viral (v erb b) oncogene homolog antibody
    • Avian erythroblastic leukemia viral (verbb) oncogene homolog antibody
    • Cell growth inhibiting protein 40 antibody
    • Cell proliferation inducing protein 61 antibody
    • EGF R antibody
    • EGFR antibody
    • EGFR antibody
    • EGFR_HUMAN antibody
    • Epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog) antibody
    • Epidermal growth factor receptor (erythroblastic leukemia viral (v erb b) oncogene homolog avian) antibody
    • Epidermal growth factor receptor antibody
    • erb-b2 receptor tyrosine kinase 1 antibody
    • erbb 1 antibody
    • Erbb antibody
    • Erbb1 antibody
    • ERBB1 antibody
    • Errp antibody
    • HER1 antibody
    • mENA antibody
    • NISBD2 antibody
    • Oncogen ERBB antibody
    • Oncogene ERBB antibody
    • PIG61 antibody
    • Proto-oncogene c-ErbB-1 antibody
    • Receptor tyrosine protein kinase ErbB 1 antibody
    • Receptor tyrosine protein kinase ErbB1 antibody
    • Receptor tyrosine-protein kinase ErbB-1 antibody
    • SA7 antibody
    • Species antigen 7 antibody
    • Urogastrone antibody
    • v-erb-b Avian erythroblastic leukemia viral oncogen homolog antibody
    • wa2 antibody
    • Wa5 antibody
    see all

Anti-EGFR antibody [ICR10] (FITC) images

  • ab11400 (1/10 dilution) staining A431 cells by Flow Cytometry. Green - isotype control; Red - ab11400.

References for Anti-EGFR antibody [ICR10] (FITC) (ab11400)

This product has been referenced in:
  • Belmont PJ  et al. Resistance to dual blockade of the kinases PI3K and mTOR in KRAS-mutant colorectal cancer models results in combined sensitivity to inhibition of the receptor tyrosine kinase EGFR. Sci Signal 7:ra107 (2014). Read more (PubMed: 25389372) »
  • Doan PL  et al. Epidermal growth factor regulates hematopoietic regeneration after radiation injury. Nat Med 19:295-304 (2013). Mouse . Read more (PubMed: 23377280) »

See all 3 Publications for this product

Product Wall

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab11400.

To check the status of the order please contact o...

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Thank you for contacting us.

The only isotype control we can offer is ab18446;

Please note that the concentration of isotype is not known be...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Mouse Cell (Balb3T3)
Specification Balb3T3
Preparation Cell harvesting/tissue preparation method: Cultured in DMEM and removed from plate with trypsin/EDTA
Sample buffer: PBS with 0.1% BSA
Fixation none
Permeabilization No
Gating Strategy forward scatter and side scatter

Abcam user community

Verified customer

Submitted May 03 2011

Thank you for your enquiry. The exact epitope sequence recognised by this antibody is not known at the present time. We know that it has been raised against the extracellular domain for the human EGFR and is directed against epitope B (epitopes A-E, ...

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