Anti-EGFR antibody [ICR10] (FITC) (ab11400)

Overview

  • Product nameAnti-EGFR antibody [ICR10] (FITC)
    See all EGFR primary antibodies
  • Description
    Rat monoclonal [ICR10] to EGFR (FITC)
  • ConjugationFITC. Ex: 493nm, Em: 528nm
  • Tested applicationsFlow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    The extracellular domain of human EGFR from head and neck carcinoma.

  • EpitopeBinds Epitope B with an affinity of 6.7 x 10-9 M.
  • General notesThis product should be stored undiluted. Storage in frost-free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.

Properties

Applications

Our Abpromise guarantee covers the use of ab11400 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10. Use 10ul of the suggested working dilution to label 106cells in 100ul.



ab18446-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Target

  • Information by UniProt
  • Database links
  • Alternative names
    • Avian erythroblastic leukemia viral (v erb b) oncogene homolog antibody
    • Cell growth inhibiting protein 40 antibody
    • Cell proliferation inducing protein 61 antibody
    • EGF R antibody
    • EGFR antibody
    • EGFR_HUMAN antibody
    • Epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog) antibody
    • Epidermal growth factor receptor (erythroblastic leukemia viral (v erb b) oncogene homolog avian) antibody
    • Epidermal growth factor receptor antibody
    • erb-b2 receptor tyrosine kinase 1 antibody
    • ERBB antibody
    • ERBB1 antibody
    • Errp antibody
    • HER1 antibody
    • mENA antibody
    • NISBD2 antibody
    • Oncogen ERBB antibody
    • PIG61 antibody
    • Proto-oncogene c-ErbB-1 antibody
    • Receptor tyrosine protein kinase ErbB 1 antibody
    • Receptor tyrosine-protein kinase ErbB-1 antibody
    • SA7 antibody
    • Species antigen 7 antibody
    • Urogastrone antibody
    • v-erb-b Avian erythroblastic leukemia viral oncogen homolog antibody
    • wa2 antibody
    • Wa5 antibody
    see all

Anti-EGFR antibody [ICR10] (FITC) images

  • ab11400 (1/10 dilution) staining A431 cells by Flow Cytometry. Green - isotype control; Red - ab11400.

References for Anti-EGFR antibody [ICR10] (FITC) (ab11400)

This product has been referenced in:
  • Belmont PJ  et al. Resistance to dual blockade of the kinases PI3K and mTOR in KRAS-mutant colorectal cancer models results in combined sensitivity to inhibition of the receptor tyrosine kinase EGFR. Sci Signal 7:ra107 (2014). Read more (PubMed: 25389372) »
  • Doan PL  et al. Epidermal growth factor regulates hematopoietic regeneration after radiation injury. Nat Med 19:295-304 (2013). Mouse . Read more (PubMed: 23377280) »

See all 3 Publications for this product

Product Wall

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab11400.

To check the status of the order please contact o...

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Thank you for contacting us.

The only isotype control we can offer is ab18446; http://www.abcam.com/Rat-IgG2a-kappa-monoclonal-RTK2758-FITC-Isotype-control-ab18446.html

Please note that the concentration of isotype is not known be...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Mouse Cell (Balb3T3)
Specification Balb3T3
Preparation Cell harvesting/tissue preparation method: Cultured in DMEM and removed from plate with trypsin/EDTA
Sample buffer: PBS with 0.1% BSA
Fixation none
Permeabilization No
Gating Strategy forward scatter and side scatter
Username

Abcam user community

Verified customer

Submitted May 03 2011

Thank you for your enquiry. The exact epitope sequence recognised by this antibody is not known at the present time. We know that it has been raised against the extracellular domain for the human EGFR and is directed against epitope B (epitopes A-E, ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"