Products:Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Receptor Tyrosine Kinases
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ab231 has been referenced in 5 publications.
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Staining of A431 cells with ab231 visualised with F(ab')2 rabbit anti-rat FITC conjugated secondary.
ICC/IF image of ab231 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab231, 5µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 donkey anti-rat IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).
ab231 used undiluted in inhibition assay against human recombinant protein EGFR1:Fc to inhibit binding to EGF:Biotin. The primary incubated with sample for 1 hour. The antibody inhibits the binding of biotinylated EGF to coated EGFR:Fc with an IC50 ~ 5 nM.
ab231 staining EGFR in human tumor cell line xenograft in mouse by Immunohistochemistry (Frozen sections).Tissue was fixed in acetone. Samples incubated with ab231 at a 1/100 dilution for 1 hour at 23°C. The secondary used was an undiluted HRP conjugated goat anti-rat polyclonal.
Image courtesy of an anonymous Abreview.
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