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Anti-EGFR antibody [ICR10] (ab231)

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Overview

Product name

Anti-EGFR antibody [ICR10]
See all EGFR products (87) ...

Description

Rat monoclonal [ICR10] to EGFR

Specificity

ICR10 recognises the human epidermal growth factor receptor (EGF-R). It does not react with erb-B2.

Tested applications

Inhibition Assay, Flow Cyt, ELISA, IP, IHC-Frmore details

Cross reactivity

Reacts with

Mouse, Human

Immunogen

Extracellular domain of Human EGF-receptor from head and neck carcinoma

Epitope

ICR10 binds to epitope B, and has an affinity of 6.7x10-9 M.

Positive control

FACS: A431 cells IHC: Breast Carcinoma tissue WB: HN5 cell lysate

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.2% Sodium Azide
Constituents: PBS

Concentration

Concentration information loading...

Purity

IgG fraction

Purification notes

Purified IgG prepared by ion exchange chromotography from ascites. Contains 0.02% sodium azide as preservative.

Clonality

Monoclonal

Clone number

ICR10

Myeloma

unknown

Isotype

IgG2a

Light chain type

unknown

Research areas

Cancer >> Tumor biomarkers >> Receptors
Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Growth factor receptors
Cancer >> Signal transduction >> Protein phosphorylation >> Tyrosine kinases >> Receptor tyrosine kinases
Signal Transduction >> Growth Factors/Hormones >> EGF
Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Receptor Tyrosine Kinases

  • Flow Cytometry / FACS - EGFR antibody [ICR10] (ab231)Flow Cytometry / FACS - EGFR antibody [ICR10] (ab231) image (enlarge)

  • Immunocytochemistry/ Immunofluorescence - EGFR antibody [ICR10] (ab231)Immunocytochemistry/ Immunofluorescence - EGFR antibody [ICR10] (ab231) image (enlarge)

  • Inhibition Assay - EGFR antibody [ICR10] (ab231)Inhibition Assay - EGFR antibody [ICR10] (ab231) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab231 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

Is unsuitable for or WB.

Target

Function

Receptor for EGF, but also for other members of the EGF family, as TGF-alpha, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. Is involved in the control of cell growth and differentiation. Phosphorylates MUC1 in breast cancer cells and increases the interaction of MUC1 with SRC and CTNNB1/beta-catenin.
Isoform 2 may act as an antagonist of EGF action.

Tissue specificity

Ubiquitously expressed. Isoform 2 is also expressed in ovarian cancers.

Involvement in disease

Defects in EGFR are associated with lung cancer (LNCR) [MIM:211980].

Sequence similarities

Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
Contains 1 protein kinase domain.

Post-translational
modifications

Phosphorylation of Ser-695 is partial and occurs only if Thr-693 is phosphorylated.
Monoubiquitinated and polyubiquitinated upon EGF stimulation; which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting. Polyubiquitin linkage is mainly through 'Lys-63', but linkage through 'Lys-48', 'Lys-11' and 'Lys-29' also occur.

Cellular localization

Secreted and Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus membrane. Nucleus membrane. Endosome. In response to EGF, translocated from the cell membrane to the nucleus via Golgi and ER. Co-localizes with TNK2 on the endosomes.

Target information above from: UniProt accessionP00533 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • EGFR antibody
  • Avian erythroblastic leukemia viral (v erb b) oncogene homolog antibody
  • Avian erythroblastic leukemia viral (verbb) oncogene homolog antibody
  • Cell growth inhibiting protein 40 antibody
  • Cell proliferation inducing protein 61 antibody
  • EGF R antibody
  • EGFR antibody
  • EGFR_HUMAN antibody
  • Epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog) antibody
  • Epidermal growth factor receptor (erythroblastic leukemia viral (v erb b) oncogene homolog avian) antibody
  • Epidermal growth factor receptor antibody
  • erbb 1 antibody
  • Erbb antibody
  • Erbb1 antibody
  • ERBB1 antibody
  • HER1 antibody
  • mENA antibody
  • Oncogene ERBB antibody
  • PIG61 antibody
  • Proto-oncogene c-ErbB-1 antibody
  • Receptor tyrosine protein kinase ErbB 1 antibody
  • Receptor tyrosine protein kinase ErbB1 antibody
  • Receptor tyrosine-protein kinase ErbB-1 antibody
  • Urogastrone antibody
  • wa2 antibody
  • Wa5 antibody
see all

Database links

see all

Anti-EGFR antibody [ICR10] images:

  Flow Cytometry / FACS - EGFR antibody [ICR10] (ab231)

Flow Cytometry / FACS - EGFR antibody [ICR10] (ab231)

Staining of A431 cells with ab231 visualised with F(ab')2 rabbit anti-rat FITC conjugated secondary.

  Immunocytochemistry/ Immunofluorescence - EGFR antibody [ICR10] (ab231)

Immunocytochemistry/ Immunofluorescence - EGFR antibody [ICR10] (ab231)

ICC/IF image of ab231 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab231, 5µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 donkey anti-rat IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).

  Inhibition Assay - EGFR antibody [ICR10] (ab231)

Inhibition Assay - EGFR antibody [ICR10] (ab231)

ab231 used undiluted in inhibition assay against human recombinant protein EGFR1:Fc to inhibit binding to EGF:Biotin. The primary incubated with sample for 1 hour. The antibody inhibits the binding of biotinylated EGF to coated EGFR:Fc with an IC50 ~ 5 nM.

References for Anti-EGFR antibody [ICR10] (ab231)

This product has been referenced in:

  • Bachran Det al. Epidermal growth factor receptor expression affects the efficacy of the combined application of saponin and a targeted toxin on human cervical carcinoma cells. Int J Cancer 127:1453-61 (2010). ELISA; Human, Mouse.Read more (PubMed: 20020492) »
  • Feng Bet al. Delivery of sodium borocaptate to glioma cells using immunoliposome conjugated with anti-EGFR antibodies by ZZ-His. Biomaterials 30:1746-55 (2009).Read more (PubMed: 19121537) »

See all 5 publications for this product

Publishing research using ab231? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"