Overview

  • Product nameAnti-EGFR antibody
    See all EGFR primary antibodies
  • Description
    Rabbit polyclonal to EGFR
  • Specificityab15669 specifically detects EGFR protein in WB and IHC-Fr in mouse brain. In WB, ab15669 detects a larger MW band (200kDa) than predicted (134kDa); this is likely to be the glycosylated form of EGFR protein.
  • Tested applicationsWB, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Rat
  • Immunogen

    Synthetic peptide derived from residues 350 - 450 of Mouse EGFR.

    .

  • Positive control
    • This antibody gave a positive signal in Mouse Brain Tissue

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab15669 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 µg/ml. Detects a band of approximately 200 kDa (predicted molecular weight: 134 kDa).
IHC-Fr Use a concentration of 2 - 5 µg/ml.

Target

  • Information by UniProt
  • Database links
  • Alternative names
    • Avian erythroblastic leukemia viral (v erb b) oncogene homolog antibody
    • Cell growth inhibiting protein 40 antibody
    • Cell proliferation inducing protein 61 antibody
    • EGF R antibody
    • EGFR antibody
    • EGFR_HUMAN antibody
    • Epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog) antibody
    • Epidermal growth factor receptor (erythroblastic leukemia viral (v erb b) oncogene homolog avian) antibody
    • Epidermal growth factor receptor antibody
    • erb-b2 receptor tyrosine kinase 1 antibody
    • ERBB antibody
    • ERBB1 antibody
    • Errp antibody
    • HER1 antibody
    • mENA antibody
    • NISBD2 antibody
    • Oncogen ERBB antibody
    • PIG61 antibody
    • Proto-oncogene c-ErbB-1 antibody
    • Receptor tyrosine protein kinase ErbB 1 antibody
    • Receptor tyrosine-protein kinase ErbB-1 antibody
    • SA7 antibody
    • Species antigen 7 antibody
    • Urogastrone antibody
    • v-erb-b Avian erythroblastic leukemia viral oncogen homolog antibody
    • wa2 antibody
    • Wa5 antibody
    see all

Anti-EGFR antibody images

  • ab15669 EGFR antibody staining in E17 mouse cerebral cortex (5ug/ml). E17 mouse embryos were fixed in 4% paraformaldehyde in PB overnight at 4°C then transferred to 30% sucrose in PBS. Brains were frozen in TissueTek and stored -80°C. Cryostat coronal sections were cut at 12um. Sections were blocked in 0.1% triton in PBS containing 10% normal serum from the species that secondary antibody was generated from. Primary antibodies were incubated on the sections at 4°C overnight. Secondary antibodies were incubated for 2 hours at RT. Images were taken using a Zeiss camera under 40X objectives
  • All lanes : Anti-EGFR antibody (ab15669) at 2 µg/ml

    Lane 1 : mouse brain lysate
    Lane 2 : mouse brain lysate with Mouse EGFR peptide (ab15739) at 1 µg/ml

    Secondary
    Alexa Fluor Goat polyclonal to Rabbit IgG at 0.1 µg/ml

    Performed under reducing conditions.

    Predicted band size : 134 kDa
    Observed band size : 200 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 50 kDa (possible IgG).

References for Anti-EGFR antibody (ab15669)

This product has been referenced in:
  • Al-Nedawi K  et al. Endothelial expression of autocrine VEGF upon the uptake of tumor-derived microvesicles containing oncogenic EGFR. Proc Natl Acad Sci U S A 106:3794-9 (2009). IHC-P ; Mouse . Read more (PubMed: 19234131) »
  • Alagappan D  et al. Brain injury expands the numbers of neural stem cells and progenitors in the SVZ by enhancing their responsiveness to EGF. ASN Neuro 1: (2009). WB ; Rat . Read more (PubMed: 19570028) »

See all 3 Publications for this product

Product Wall

Thank you for your reply.

I appreciate the time that you took to try and optimize the protocol using ab2430. I am sorry to learn that this different fixation did not improve the staining.

As requested, I have issued a free of charge...

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Thank you for contacting us.

We have 46 polyclonal EGFR antibodies in our catalog, three of which are raised against the extracellular domain (residues 25 - 645):

ab15669 (http://www.abcam.com/EGFR-antibody-ab15669.html)
ab103...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"