Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab2430 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent dilution.
ICC/IF 1/20 - 1/100. PubMed: 17567803
Sandwich ELISA Use a concentration of 0.5 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal [F4] to EGFR (ab62). For sandwich ELISA, use this antibody as Detection at 0.5µg/ml with Ab62 as Capture.
ICC Use at an assay dependent dilution.
WB 1/200. Can be blocked with EGFR peptide (ab2432).
IP Use at an assay dependent dilution.
IHC-P Use at an assay dependent dilution.
IHC-Fr Use at an assay dependent dilution.
IHC-FrFl Use at an assay dependent concentration.
In-Cell ELISA Use at an assay dependent concentration. PubMed: 21536028

Target

  • FunctionReceptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin.
    Isoform 2 may act as an antagonist of EGF action.
  • Tissue specificityUbiquitously expressed. Isoform 2 is also expressed in ovarian cancers.
  • Involvement in diseaseDefects in EGFR are associated with lung cancer (LNCR) [MIM:211980]. LNCR is a common malignancy affecting tissues of the lung. The most common form of lung cancer is non-small cell lung cancer (NSCLC) that can be divided into 3 major histologic subtypes: squamous cell carcinoma, adenocarcinoma, and large cell lung cancer. NSCLC is often diagnosed at an advanced stage and has a poor prognosis.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Phosphorylation at Ser-695 is partial and occurs only if Thr-693 is phosphorylated. Phosphorylation at Thr-678 and Thr-693 by PRKD1 inhibits EGF-induced MAPK8/JNK1 activation. Dephosphorylation by PTPRJ prevents endocytosis and stabilizes the receptor at the plasma membrane. Autophosphorylation at Tyr-1197 is stimulated by methylation at Arg-1199 and enhances interaction with PTPN6. Autophosphorylation at Tyr-1092 and/or Tyr-1110 recruits STAT3.
    Monoubiquitinated and polyubiquitinated upon EGF stimulation; which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting. Polyubiquitin linkage is mainly through 'Lys-63', but linkage through 'Lys-48', 'Lys-11' and 'Lys-29' also occur. Deubiquitinated by OTUD7B, preventing degradation.
    Methylated. Methylation at Arg-1199 by PRMT5 positively stimulates phosphorylation at Tyr-1197.
  • Cellular localizationSecreted and Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus membrane. Nucleus membrane. Endosome. Endosome membrane. In response to EGF, translocated from the cell membrane to the nucleus via Golgi and ER. Endocytosed upon activation by ligand.
  • Information by UniProt
  • Database links
  • Alternative names
    • Avian erythroblastic leukemia viral (v erb b) oncogene homolog antibody
    • Avian erythroblastic leukemia viral (verbb) oncogene homolog antibody
    • Cell growth inhibiting protein 40 antibody
    • Cell proliferation inducing protein 61 antibody
    • EGF R antibody
    • EGFR antibody
    • EGFR antibody
    • EGFR_HUMAN antibody
    • Epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog) antibody
    • Epidermal growth factor receptor (erythroblastic leukemia viral (v erb b) oncogene homolog avian) antibody
    • Epidermal growth factor receptor antibody
    • erbb 1 antibody
    • Erbb antibody
    • Erbb1 antibody
    • ERBB1 antibody
    • Errp antibody
    • HER1 antibody
    • mENA antibody
    • Oncogen ERBB antibody
    • Oncogene ERBB antibody
    • PIG61 antibody
    • Proto-oncogene c-ErbB-1 antibody
    • Receptor tyrosine protein kinase ErbB 1 antibody
    • Receptor tyrosine protein kinase ErbB1 antibody
    • Receptor tyrosine-protein kinase ErbB-1 antibody
    • SA7 antibody
    • Species antigen 7 antibody
    • Urogastrone antibody
    • v-erb-b Avian erythroblastic leukemia viral oncogen homolog antibody
    • wa2 antibody
    • Wa5 antibody
    see all

Anti-EGFR antibody images

  • ab2430 at a 1/200 dilution staining A431 whole cell lysate. 
  • Cells were stained with anti-EGFR (ab2430; red) and DAPI (blue). A 1/40 dilution of ab2430 was used.HeLa cells were fixed with 4% formaldehyde in PEM buffer. The coverslip was incubated in blocking buffer of 5% powdered milk in TBS-T plus 0.02% sodium azide for 1 hour at room temperature. Blocking buffer was removed and primary antibody was added at a dilution of 1/40 and incubated overnight at 4 degrees celsius. The coverslips were then washed 4-5 times with blocking buffer for 5 minutes. Secondary antibody was added at a dilution of 1/1000 and incubated at room temperature for one hour. From this point on coverslips were covered with foil to protect them from light. They were washed 5 times with TBS-T and then one time with PEM, for 5 minutes each wash. The coverslips were fixed 10-30 minutes in 4% formaldehyde in PEM buffer, then washed 3 times with PEM buffer for 5 minutes. 0.1M ammonium chloride in PEM buffer was added for 10 minutes to quench auto-florescence, and then slips were washed 2 times for 5 minutes in PEM followed by 3 washes for 5 minutes in TBS-T. Coverslips were then counterstained with DAPI in TBS-T for 1-2 minutes, TBS-T was then added and the coverslips mounted.
  • ab2430 staining formalin fixed paraffin-embedded sections of human adhesion tissue.  The section was subjected to heat-mediated antigen retrieval in citrate buffer (pH 6.0) and blocked with 10% BSA for 1 hour prior to incubating with the primary antibody (diluted 1/200) for 12 hours at 4°C.  A biotinylated goat anti-rabbit antibody was used as the secondary.

    See Abreview

  • ab2430 staining EGFR in human stomach tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent formaldehyde fixation before heat mediated antigen retrieval in 10 mM Citrate pH 6.0 and then blocking with 5% serum for 1 hour at 23°C was performed. The primary antibody was diluted 1/100 and incubated with sample for 1 hour at 23°C. A HRP conjugated goat polyclonal to rabbit IgG was used undiluted as secondary antibody.

    See Abreview

  • ab2430 immunoprecipitate of EGFR in human AGS whole cell lysate. 100 µg of cell lysate was incubated with primary antibody (1/100 in RIPA buffer) and matrix (Protein A/G) for 16 hours at 4°C.
    For western blotting Ab6721 (1/1000) was used.

    See Abreview

  • Standard Curve for EGFR (Analyte: ab61264)dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [F4] to EGFR (ab62) at 5ug/ml and Detector Antibody Rabbit polyclonal to EGFR (ab2430) at 0.5ug/ml

References for Anti-EGFR antibody (ab2430)

This product has been referenced in:
  • Harrison H  et al. Oestrogen increases the activity of oestrogen receptor negative breast cancer stem cells through paracrine EGFR and Notch signalling. Breast Cancer Res 15:R21 (2013). WB ; Human . Read more (PubMed: 23497505) »
  • Tzouvelekis A  et al. Increased expression of epidermal growth factor receptor (EGF-R) in patients with different forms of lung fibrosis. Biomed Res Int 2013:654354 (2013). IHC ; Human . Read more (PubMed: 23841084) »

See all 25 Publications for this product

Product Wall

Here is a more complete description of the EGFR fragment ab68104:

The EGFR contains the extracellular domain of the human EGFR (25-647 a.a.) excluding the signal peptide.

So, ab2430 and ab62 are not appropriate choices for detecti...

Read More

Thank you for contacting us and for your patience.

We were looking for negative controls for EGFR. The EGFR is ubiquitously expressed and regrettably we were not able to find a good negative control to suggest on this occasion. However, we fou...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa)
Loading amount 50 µg
Specification HeLa
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Nov 06 2012

All of our EGFR proteins are recombinant fragments, and none are full-length, with the exception of ab132920. However, this is the truncated 405 amino acid isoform 2, not the larger 1,210 aa isoform 1. Descriptions of the isoforms of EGFR can be found ...

Read More

Thank you for contacting us and your interest in our products.

The anti-EGFR antibody (ab2430) has been raised against a peptide taken from the region 1005-1016 of EGFR. I have checked from the SwissProt reference P00533 and could not find any...

Read More

Can you please let me know whether the supplementary information we had sent in Julyresolvedthe problems withab2430? If yes, we would be happy to be able to update our records. Otherwise we would be pleased to offer you a replacement with an alternativ...

Read More

Thank you for your e-mail.

This is to confirm that your Abreview (Id:30802) regarding ab2430 - Anti-EGFR antibody has been published on-line and the discount code is activated.

If you need any further assistance in the future, pleas...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Sheep Tissue sections (Lung)
Specification Lung
Fixative Ethanol
Permeabilization No
Blocking step !% BSA / 5% Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jul 24 2012

Thank you for your message and for providing this further information.

I am sorry to hear the suggestions made have not improved the results on this occasion. I appreciate the time your customer hasspent on these experiments and would be pleas...

Read More

Thank you for contacting us.
please find the information that we just received from the source of the antibody:

"We are not sure how this antibody reacts with cytoplasmic fractions. We have always used whole cell lysates. There might ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"