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Western blot - EGFR antibody (ab2430)
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Immunofluorescence - EGFR antibody (ab2430)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - EGFR antibody (ab2430)
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Product Name
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EGFR antibody
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See all EGFR antibodies (78)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to EGFR
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Immunogen
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Synthetic peptide: EDMDDVVDADEY, corresponding to C terminal amino acids 1005-1016 of EGFR.
BLAST 'EDMDDVVDADEY' with  or 
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Reacts with
(species key)
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Hu, Ms, Rat, Dog
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Tested applications
(see key)
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ICC/IF, IHC-Fr, IHC-P, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: 1/20 - 1/100(from PMID 17567803).
IHC-P: Use at an assay dependent concentration.
IHC-Fr: Use at an assay dependent concentration.
IP: Use at an assay dependent concentration.
WB: 1/200.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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A-431 lysate.
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Cellular localization
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Type I membrane protein (isoform 1) and secreted (isoform 2).
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Research areas
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Cancer >> Tumor biomarkers >> Receptors
Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Growth factor receptors
Cancer >> Signal transduction >> Protein phosphorylation >> Tyrosine kinases >> Receptor tyrosine kinases
Signal Transduction >> Growth Factors/Hormones >> EGF
Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Receptor Tyrosine Kinases
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Relevance
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Protein kinases are enzymes that transfer a phosphate group from a phosphate donor onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. The protein kinase family is one of the largest families of proteins in eukaryotes, classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. Epidermal Growth factor receptor (EGFR) is the prototype member of the type 1 receptor tyrosine kinases. EGFR overexpression in tumors indicates poor prognosis and is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, stomach and in squamous cell carcinoma.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Storage buffer
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Preservative: 0.1% Sodium Azide
Constituents: 0.2% Gelatin, PBS Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.200 mg/ml
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Storage instructions
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Store at +4°C. Do not freeze.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this EGFR antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for EGFR antibody images, references, products related to ab2430 and other tools.
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EGFR antibody images:
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 Western blot - EGFR antibody (ab2430)
ab2430 at a 1/200 dilution staining A431 whole cell lysate.
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Immunofluorescence - EGFR antibody (ab2430)
Cells were stained with anti-EGFR (ab2430; red) and DAPI (blue). A 1/40 dilution of ab2430 was used.
HeLa cells were fixed with 4% formaldehyde in PEM buffer. The coverslip was incubated in blocking buffer of 5% powdered milk in TBS-T plus 0.02% sodium azide for 1 hour at room temperature. Blocking buffer was removed and primary antibody was added at a dilution of 1/40 and incubated overnight at 4 degrees celsius. The coverslips were then washed 4-5 times with blocking buffer for 5 minutes. Secondary antibody was added at a dilution of 1/1000 and incubated at room temperature for one hour. From this point on coverslips were covered with foil to protect them from light. They were washed 5 times with TBS-T and then one time with PEM, for 5 minutes each wash. The coverslips were fixed 10-30 minutes in 4% formaldehyde in PEM buffer, then washed 3 times with PEM buffer for 5 minutes. 0.1M ammonium chloride in PEM buffer was added for 10 minutes to quench auto-florescence, and then slips were washed 2 times for 5 minutes in PEM followed by 3 washes for 5 minutes in TBS-T. Coverslips were then counterstained with DAPI in TBS-T for 1-2 minutes, TBS-T was then added and the coverslips mounted. Michael Mancini, Ph.D.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - EGFR antibody (ab2430)
ab2430 staining formalin fixed paraffin-embedded sections of human adhesion tissue. The section was subjected to heat-mediated antigen retrieval in citrate buffer (pH 6.0) and blocked with 10% BSA for 1 hour prior to incubating with the primary antibody (diluted 1/200) for 12 hours at 4°C. A biotinylated goat anti-rabbit antibody was used as the secondary. This image is courtesy of an anonymous Abreview
See Abreview
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References for EGFR antibody (ab2430)
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This product has been used in: (two most recent references)
Qayum N et al. Tumor vascular changes mediated by inhibition of oncogenic signaling. Cancer Res 69:6347-54 (2009). IHC. PubMed: 19622766
Vlotides G et al. Fibroblast growth factor-2 autofeedback regulation in pituitary folliculostellate TtT/GF cells. Endocrinology 150:3252-8 (2009). PubMed: 19359387
See all 10 publication references for this product.
If you publish research using ab2430 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to EGFR
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EGFR antibody - more information
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Customer reviews (feedback) regarding EGFR antibody |
Customer FAQs regarding EGFR antibody |
Protocols for EGFR antibody |
Price and availability of products related to EGFR antibody |
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EGFR antibody - related products:
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EGFR antibody - other tools:
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Contact Abcam with a Technical Enquiry about EGFR antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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