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Synthetic peptide: EDMDDVVDADEY, corresponding to C terminal amino acids 1005-1016 of EGFR.
Our Abpromise guarantee covers the use of ab2430 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent dilution.|
|ICC/IF||1/20 - 1/100. PubMed: 17567803|
|Sandwich ELISA||Use a concentration of 0.5 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal [F4] to EGFR (ab62). For sandwich ELISA, use this antibody as Detection at 0.5µg/ml with Ab62 as Capture.|
|ICC||Use at an assay dependent dilution.|
|WB||1/200. Can be blocked with EGFR peptide (ab2432).|
|IP||Use at an assay dependent dilution.|
|IHC-P||Use at an assay dependent dilution.|
|IHC-Fr||Use at an assay dependent dilution.|
|IHC-FrFl||Use at an assay dependent concentration.|
|In-Cell ELISA||Use at an assay dependent concentration. PubMed: 21536028|
ab2430 staining formalin fixed paraffin-embedded sections of human adhesion tissue. The section was subjected to heat-mediated antigen retrieval in citrate buffer (pH 6.0) and blocked with 10% BSA for 1 hour prior to incubating with the primary antibody (diluted 1/200) for 12 hours at 4°C. A biotinylated goat anti-rabbit antibody was used as the secondary.
ab2430 staining EGFR in human stomach tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent formaldehyde fixation before heat mediated antigen retrieval in 10 mM Citrate pH 6.0 and then blocking with 5% serum for 1 hour at 23°C was performed. The primary antibody was diluted 1/100 and incubated with sample for 1 hour at 23°C. A HRP conjugated goat polyclonal to rabbit IgG was used undiluted as secondary antibody.
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