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Western blot - EGFR antibody (ab2430)
Western blot - EGFR antibody (ab2430)
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Immunofluorescence - EGFR antibody (ab2430)
Immunofluorescence - EGFR antibody (ab2430)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - EGFR antibody (ab2430)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - EGFR antibody (ab2430)
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Product Name 

EGFR antibody

 

See all EGFR antibodies (78)...

Product type 

Primary antibodies

Description 

Rabbit polyclonal to EGFR

Immunogen 

Synthetic peptide: EDMDDVVDADEY, corresponding to C terminal amino acids 1005-1016 of EGFR.
BLAST 'EDMDDVVDADEY' with BLAST the sequence with ExPASy or BLAST the sequence with NCBI

Reacts with 
(species key)

Hu, Ms, Rat, Dog

Tested applications 
(see key)

ICC/IF, IHC-Fr, IHC-P, IP, WB


Abreviews 

Customer reviews
(see key)


Read about Abreviews
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• ICC

1 Abreview

- average rating: 4 stars (out of 5)4 stars (out of 5)4 stars (out of 5)4 stars (out of 5)4 stars (out of 5)

• ICC/IF

1 Abreview

- average rating: 4 stars (out of 5)4 stars (out of 5)4 stars (out of 5)4 stars (out of 5)4 stars (out of 5)

• IHC-P

6 Abreviews

- average rating: 4 stars (out of 5)4 stars (out of 5)4 stars (out of 5)4 stars (out of 5)4 stars (out of 5)

• IHC-Fr

1 Abreview

 

• WB

5 Abreviews

- average rating: 4 stars (out of 5)4 stars (out of 5)4 stars (out of 5)4 stars (out of 5)4 stars (out of 5)


ICC/IF
(read Abreview)


IHC-P
(read Abreview)


ICC
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WB
(read Abreview)


Application notes 
(see key)

Recommended dilutions
ICC/IF: 1/20 - 1/100(from PMID 17567803).
IHC-P: Use at an assay dependent concentration.
IHC-Fr: Use at an assay dependent concentration.
IP: Use at an assay dependent concentration.
WB: 1/200.



Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

A-431 lysate.

Cellular localization 

Type I membrane protein (isoform 1) and secreted (isoform 2).

Research areas 

Cancer >> Tumor biomarkers >> Receptors
Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Growth factor receptors
Cancer >> Signal transduction >> Protein phosphorylation >> Tyrosine kinases >> Receptor tyrosine kinases
Signal Transduction >> Growth Factors/Hormones >> EGF
Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Receptor Tyrosine Kinases

Relevance 

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. The protein kinase family is one of the largest families of proteins in eukaryotes, classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. Epidermal Growth factor receptor (EGFR) is the prototype member of the type 1 receptor tyrosine kinases. EGFR overexpression in tumors indicates poor prognosis and is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, stomach and in squamous cell carcinoma.

 

Alternative names EGFR antibody (ab2430)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

404306    (Dog)
1956    (Human)
13649    (Mouse)
24329    (Rat)

Omim

    

131550    (Human)

SwissProt

    

P00533    (Human)
Q01279    (Mouse)
Q9QX70    (Rat)

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Storage buffer 

Preservative: 0.1% Sodium Azide
Constituents: 0.2% Gelatin, PBS

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

0.200 mg/ml

Storage instructions 

Store at +4°C. Do not freeze.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this EGFR antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for EGFR antibody images, references, products related to ab2430 and other tools.

 

EGFR antibody images:

Product image  Western blot - EGFR antibody (ab2430)

Western blot - EGFR antibody (ab2430)

ab2430 at a 1/200 dilution staining A431 whole cell lysate. 

 

Product image  Immunofluorescence - EGFR antibody (ab2430)

Immunofluorescence - EGFR antibody (ab2430)

Cells were stained with anti-EGFR (ab2430; red) and DAPI (blue). A 1/40 dilution of ab2430 was used.

HeLa cells were fixed with 4% formaldehyde in PEM buffer. The coverslip was incubated in blocking buffer of 5% powdered milk in TBS-T plus 0.02% sodium azide for 1 hour at room temperature. Blocking buffer was removed and primary antibody was added at a dilution of 1/40 and incubated overnight at 4 degrees celsius. The coverslips were then washed 4-5 times with blocking buffer for 5 minutes. Secondary antibody was added at a dilution of 1/1000 and incubated at room temperature for one hour. From this point on coverslips were covered with foil to protect them from light. They were washed 5 times with TBS-T and then one time with PEM, for 5 minutes each wash. The coverslips were fixed 10-30 minutes in 4% formaldehyde in PEM buffer, then washed 3 times with PEM buffer for 5 minutes. 0.1M ammonium chloride in PEM buffer was added for 10 minutes to quench auto-florescence, and then slips were washed 2 times for 5 minutes in PEM followed by 3 washes for 5 minutes in TBS-T. Coverslips were then counterstained with DAPI in TBS-T for 1-2 minutes, TBS-T was then added and the coverslips mounted.

Michael Mancini, Ph.D.

 

Product image  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - EGFR antibody (ab2430)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - EGFR antibody (ab2430)

ab2430 staining formalin fixed paraffin-embedded sections of human adhesion tissue.  The section was subjected to heat-mediated antigen retrieval in citrate buffer (pH 6.0) and blocked with 10% BSA for 1 hour prior to incubating with the primary antibody (diluted 1/200) for 12 hours at 4°C.  A biotinylated goat anti-rabbit antibody was used as the secondary.

This image is courtesy of an anonymous Abreview

See Abreview

 

References for EGFR antibody (ab2430)

Specific publication references   This product has been used in: (two most recent references)

Qayum N et al. Tumor vascular changes mediated by inhibition of oncogenic signaling. Cancer Res 69:6347-54 (2009). IHC. PubMed: 19622766

Vlotides G et al. Fibroblast growth factor-2 autofeedback regulation in pituitary folliculostellate TtT/GF cells. Endocrinology 150:3252-8 (2009). PubMed: 19359387


See all 10 publication references for this product.


If you publish research using ab2430 please let us know so that we can cite the reference on this datasheet.

 

Search PubMed (MEDLINE) for references to EGFR

 

EGFR antibody - more information

Customer reviews (feedback) regarding EGFR antibody

Customer FAQs regarding EGFR antibody

Protocols for EGFR antibody

Price and availability of products related to EGFR antibody

 

EGFR antibody - related products:

Products for Immunocytochemistry

Subcellular Markers

Products for Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Slides

Products for Western blot

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