Anti-EGFR antibody (ab2430)

Overview

• Product nameAnti-EGFR antibodySee all EGFR primary antibodies ...
• Description
  Rabbit polyclonal to EGFR
• Tested applicationsFlow Cyt, ICC/IF, Sandwich ELISA, ICC, WB, IP, IHC-P, IHC-Fr, IHC-FrFl, In-Cell ELISA more details
• Species reactivity
  Reacts with: Mouse, Rat, Sheep, Cow, Dog, Human
  
• Immunogen

  Synthetic peptide: EDMDDVVDADEY, corresponding to C terminal amino acids 1005-1016 of EGFR.

  Run BLAST with Run BLAST with
• Positive controlA-431 lysate.

Properties

• FormLiquid
• Storage instructionsStore at +4°C. Do not freeze.
• Storage bufferPreservative: 0.1% Sodium Azide
  Constituents: 0.2% Gelatin, PBS
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Cancer
  • Tumor biomarkers
  • Receptors

  • Cancer
  • Oncoproteins/suppressors
  • Oncoproteins
  • Growth factor receptors

  • Cancer
  • Signal transduction
  • Protein phosphorylation
  • Tyrosine kinases
  • Receptor tyrosine kinases

  • Signal Transduction
  • Protein Phosphorylation
  • Tyrosine Kinases
  • Receptor Tyrosine Kinases

  • Signal Transduction
  • Growth Factors/Hormones
  • EGF

Applications

Target

• FunctionReceptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin.
  Isoform 2 may act as an antagonist of EGF action.
• Tissue specificityUbiquitously expressed. Isoform 2 is also expressed in ovarian cancers.
• Involvement in diseaseDefects in EGFR are associated with lung cancer (LNCR) [MIM:211980]. LNCR is a common malignancy affecting tissues of the lung. The most common form of lung cancer is non-small cell lung cancer (NSCLC) that can be divided into 3 major histologic subtypes: squamous cell carcinoma, adenocarcinoma, and large cell lung cancer. NSCLC is often diagnosed at an advanced stage and has a poor prognosis.
• Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
  Contains 1 protein kinase domain.
• Post-translational
  modificationsPhosphorylation at Ser-695 is partial and occurs only if Thr-693 is phosphorylated. Phosphorylation at Thr-678 and Thr-693 by PRKD1 inhibits EGF-induced MAPK8/JNK1 activation. Dephosphorylation by PTPRJ prevents endocytosis and stabilizes the receptor at the plasma membrane. Autophosphorylation at Tyr-1197 is stimulated by methylation at Arg-1199 and enhances interaction with PTPN6. Autophosphorylation at Tyr-1092 and/or Tyr-1110 recruits STAT3.
  Monoubiquitinated and polyubiquitinated upon EGF stimulation; which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting. Polyubiquitin linkage is mainly through 'Lys-63', but linkage through 'Lys-48', 'Lys-11' and 'Lys-29' also occur. Deubiquitinated by OTUD7B, preventing degradation.
  Methylated. Methylation at Arg-1199 by PRMT5 positively stimulates phosphorylation at Tyr-1197.
• Cellular localizationSecreted and Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus membrane. Nucleus membrane. Endosome. Endosome membrane. In response to EGF, translocated from the cell membrane to the nucleus via Golgi and ER. Endocytosed upon activation by ligand.

Target information above from: UniProt accession P00533 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 407217 Cow
  • Entrez Gene: 404306 Dog
  • Entrez Gene: 1956 Human
  • Entrez Gene: 13649 Mouse
  • Entrez Gene: 24329 Rat
  • Omim: 131550 Human
  • SwissProt: P00533 Human
  • SwissProt: Q01279 Mouse

  • Unigene: 488293 Human
  • Unigene: 420648 Mouse
  • Unigene: 439882 Mouse
  • Unigene: 8534 Mouse
  • Unigene: 37227 Rat

  see all

• Alternative names
  Avian erythroblastic leukemia viral (v erb b) oncogene homolog antibodyAvian erythroblastic leukemia viral (verbb) oncogene homolog antibodyCell growth inhibiting protein 40 antibody

  Cell proliferation inducing protein 61 antibodyEGF R antibodyEGFR antibodyEGFR antibodyEGFR_HUMAN antibodyEpidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog) antibodyEpidermal growth factor receptor (erythroblastic leukemia viral (v erb b) oncogene homolog avian) antibodyEpidermal growth factor receptor antibodyerbb 1 antibodyErbb antibodyErbb1 antibodyERBB1 antibodyErrp antibodyHER1 antibodymENA antibodyOncogene ERBB antibodyPIG61 antibodyProto-oncogene c-ErbB-1 antibodyReceptor tyrosine protein kinase ErbB 1 antibodyReceptor tyrosine protein kinase ErbB1 antibodyReceptor tyrosine-protein kinase ErbB-1 antibodyUrogastrone antibodywa2 antibodyWa5 antibody

  see all

Anti-EGFR antibody images

• 

  Western blot - EGFR antibody (ab2430)
  ab2430 at a 1/200 dilution staining A431 whole cell lysate. 
• 

  Immunofluorescence - EGFR antibody (ab2430)Michael Mancini, Ph.D.
  Cells were stained with anti-EGFR (ab2430; red) and DAPI (blue). A 1/40 dilution of ab2430 was used.HeLa cells were fixed with 4% formaldehyde in PEM buffer. The coverslip was incubated in blocking buffer of 5% powdered milk in TBS-T plus 0.02% sodium azide for 1 hour at room temperature. Blocking buffer was removed and primary antibody was added at a dilution of 1/40 and incubated overnight at 4 degrees celsius. The coverslips were then washed 4-5 times with blocking buffer for 5 minutes. Secondary antibody was added at a dilution of 1/1000 and incubated at room temperature for one hour. From this point on coverslips were covered with foil to protect them from light. They were washed 5 times with TBS-T and then one time with PEM, for 5 minutes each wash. The coverslips were fixed 10-30 minutes in 4% formaldehyde in PEM buffer, then washed 3 times with PEM buffer for 5 minutes. 0.1M ammonium chloride in PEM buffer was added for 10 minutes to quench auto-florescence, and then slips were washed 2 times for 5 minutes in PEM followed by 3 washes for 5 minutes in TBS-T. Coverslips were then counterstained with DAPI in TBS-T for 1-2 minutes, TBS-T was then added and the coverslips mounted.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - EGFR antibody (ab2430)This image is courtesy of an anonymous Abreview

  ab2430 staining formalin fixed paraffin-embedded sections of human adhesion tissue.  The section was subjected to heat-mediated antigen retrieval in citrate buffer (pH 6.0) and blocked with 10% BSA for 1 hour prior to incubating with the primary antibody (diluted 1/200) for 12 hours at 4°C.  A biotinylated goat anti-rabbit antibody was used as the secondary.

  See Abreview

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - EGFR antibody (ab2430)This image is a courtesy of Anonymous Abreview

  ab2430 staining EGFR in human stomach tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent formaldehyde fixation before heat mediated antigen retrieval in 10 mM Citrate pH 6.0 and then blocking with 5% serum for 1 hour at 23°C was performed. The primary antibody was diluted 1/100 and incubated with sample for 1 hour at 23°C. A HRP conjugated goat polyclonal to rabbit IgG was used undiluted as secondary antibody.

  See Abreview

• 

  Immunoprecipitation - EGFR antibody (ab2430)This image is courtesy of an anonymous Abreview
  ab2430 immunoprecipitate of EGFR in human AGS whole cell lysate. 100 µg of cell lysate was incubated with primary antibody (1/100 in RIPA buffer) and matrix (Protein A/G) for 16 hours at 4°C.
  For western blotting Ab6721 (1/1000) was used.

  See Abreview

• 

  Sandwich ELISA - EGFR antibody (ab2430)
  Standard Curve for EGFR (Analyte: ab61264)dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [F4] to EGFR (ab62) at 5ug/ml and Detector Antibody Rabbit polyclonal to EGFR (ab2430) at 0.5ug/ml