Overview

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab2430 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF 1/20 - 1/100. PubMed: 17567803
Sandwich ELISA Use a concentration of 0.5 µg/ml. For sandwich ELISA, use this antibody as Detection at 0.5µg/ml with Ab62 as Capture.
WB 1/200.
IP Use at an assay dependent concentration.
In-Cell ELISA Use at an assay dependent concentration. PubMed: 21536028

Target

  • Information by UniProt
  • Database links
  • Alternative names
    • Avian erythroblastic leukemia viral (v erb b) oncogene homolog antibody
    • Cell growth inhibiting protein 40 antibody
    • Cell proliferation inducing protein 61 antibody
    • EGF R antibody
    • EGFR antibody
    • EGFR_HUMAN antibody
    • Epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog) antibody
    • Epidermal growth factor receptor (erythroblastic leukemia viral (v erb b) oncogene homolog avian) antibody
    • Epidermal growth factor receptor antibody
    • erb-b2 receptor tyrosine kinase 1 antibody
    • ERBB antibody
    • ERBB1 antibody
    • Errp antibody
    • HER1 antibody
    • mENA antibody
    • NISBD2 antibody
    • Oncogen ERBB antibody
    • PIG61 antibody
    • Proto-oncogene c-ErbB-1 antibody
    • Receptor tyrosine protein kinase ErbB 1 antibody
    • Receptor tyrosine-protein kinase ErbB-1 antibody
    • SA7 antibody
    • Species antigen 7 antibody
    • Urogastrone antibody
    • v-erb-b Avian erythroblastic leukemia viral oncogen homolog antibody
    • wa2 antibody
    • Wa5 antibody
    see all

Anti-EGFR antibody images

  • ab2430 at a 1/200 dilution staining A431 whole cell lysate. 
  • Cells were stained with anti-EGFR (ab2430; red) and DAPI (blue). A 1/40 dilution of ab2430 was used.HeLa cells were fixed with 4% formaldehyde in PEM buffer. The coverslip was incubated in blocking buffer of 5% powdered milk in TBS-T plus 0.02% sodium azide for 1 hour at room temperature. Blocking buffer was removed and primary antibody was added at a dilution of 1/40 and incubated overnight at 4 degrees celsius. The coverslips were then washed 4-5 times with blocking buffer for 5 minutes. Secondary antibody was added at a dilution of 1/1000 and incubated at room temperature for one hour. From this point on coverslips were covered with foil to protect them from light. They were washed 5 times with TBS-T and then one time with PEM, for 5 minutes each wash. The coverslips were fixed 10-30 minutes in 4% formaldehyde in PEM buffer, then washed 3 times with PEM buffer for 5 minutes. 0.1M ammonium chloride in PEM buffer was added for 10 minutes to quench auto-florescence, and then slips were washed 2 times for 5 minutes in PEM followed by 3 washes for 5 minutes in TBS-T. Coverslips were then counterstained with DAPI in TBS-T for 1-2 minutes, TBS-T was then added and the coverslips mounted.
  • ab2430 staining formalin fixed paraffin-embedded sections of human adhesion tissue.  The section was subjected to heat-mediated antigen retrieval in citrate buffer (pH 6.0) and blocked with 10% BSA for 1 hour prior to incubating with the primary antibody (diluted 1/200) for 12 hours at 4°C.  A biotinylated goat anti-rabbit antibody was used as the secondary.

    See Abreview

  • ab2430 staining EGFR in human stomach tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent formaldehyde fixation before heat mediated antigen retrieval in 10 mM Citrate pH 6.0 and then blocking with 5% serum for 1 hour at 23°C was performed. The primary antibody was diluted 1/100 and incubated with sample for 1 hour at 23°C. A HRP conjugated goat polyclonal to rabbit IgG was used undiluted as secondary antibody.

    See Abreview

  • ab2430 immunoprecipitate of EGFR in human AGS whole cell lysate. 100 µg of cell lysate was incubated with primary antibody (1/100 in RIPA buffer) and matrix (Protein A/G) for 16 hours at 4°C.
    For western blotting Ab6721 (1/1000) was used.

    See Abreview

  • Standard Curve for EGFR (Analyte: ab61264)dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [F4] to EGFR (ab62) at 5ug/ml and Detector Antibody Rabbit polyclonal to EGFR (ab2430) at 0.5ug/ml

References for Anti-EGFR antibody (ab2430)

This product has been referenced in:
  • Pan Y  et al. The expression of epidermal growth factor (EGF) and its receptor (EGFR) during post-natal testes development in the yak. Reprod Domest Anim 49:970-6 (2014). IHC . Read more (PubMed: 25263304) »
  • Harrison H  et al. Oestrogen increases the activity of oestrogen receptor negative breast cancer stem cells through paracrine EGFR and Notch signalling. Breast Cancer Res 15:R21 (2013). WB ; Human . Read more (PubMed: 23497505) »

See all 26 Publications for this product

Product Wall

Application Western blot
Loading amount 40 µg
Gel Running Conditions Reduced Denaturing (7.5%)
Sample Mouse Cell lysate - whole cell (brain)
Specification brain
Treatment EGF/FGF (20 ng/ml)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Dr. Robert Kupp

Verified customer

Submitted Mar 11 2015

Here is a more complete description of the EGFR fragment ab68104:

The EGFR contains the extracellular domain of the human EGFR (25-647 a.a.) excluding the signal peptide.

So, ab2430 and ab62 are not appropriate choices for detecti...

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Thank you for contacting us and for your patience.

We were looking for negative controls for EGFR. The EGFR is ubiquitously expressed and regrettably we were not able to find a good negative control to suggest on this occasion. However, we fou...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa)
Loading amount 50 µg
Specification HeLa
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Nov 06 2012

All of our EGFR proteins are recombinant fragments, and none are full-length, with the exception of ab132920. However, this is the truncated 405 amino acid isoform 2, not the larger 1,210 aa isoform 1. Descriptions of the isoforms of EGFR can be found ...

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Thank you for contacting us and your interest in our products.

The anti-EGFR antibody (ab2430) has been raised against a peptide taken from the region 1005-1016 of EGFR. I have checked from the SwissProt reference P00533 and could not find any...

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Can you please let me know whether the supplementary information we had sent in Julyresolvedthe problems withab2430? If yes, we would be happy to be able to update our records. Otherwise we would be pleased to offer you a replacement with an alternativ...

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Thank you for your e-mail.

This is to confirm that your Abreview (Id:30802) regarding ab2430 - Anti-EGFR antibody has been published on-line and the discount code is activated.

If you need any further assistance in the future, pleas...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Sheep Tissue sections (Lung)
Specification Lung
Fixative Ethanol
Permeabilization No
Blocking step !% BSA / 5% Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jul 24 2012

Thank you for your message and for providing this further information.

I am sorry to hear the suggestions made have not improved the results on this occasion. I appreciate the time your customer hasspent on these experiments and would be pleas...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"