Overview

  • Product nameAnti-EGFR antibody
    See all EGFR primary antibodies
  • Description
    Rabbit polyclonal to EGFR
  • SpecificityThis antibody detects endogenous levels of total EGFR protein.
  • Tested applicationsELISA, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthesized non-phosphopeptide derived from human EGFR around the phosphorylation site of tyrosine 869 (K-E-Y-H-A).

  • Positive control
    • WB: A431 cells extracts. IHC-P: Human breast carcinoma tissue.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab47439 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/10000.
IHC-P Use at an assay dependent concentration.
WB 1/500 - 1/1000. Detects a band of approximately 170 kDa (predicted molecular weight: 134 kDa).

Target

  • Information by UniProt
  • Database links
  • Alternative names
    • Avian erythroblastic leukemia viral (v erb b) oncogene homolog antibody
    • Cell growth inhibiting protein 40 antibody
    • Cell proliferation inducing protein 61 antibody
    • EGF R antibody
    • EGFR antibody
    • EGFR_HUMAN antibody
    • Epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog) antibody
    • Epidermal growth factor receptor (erythroblastic leukemia viral (v erb b) oncogene homolog avian) antibody
    • Epidermal growth factor receptor antibody
    • erb-b2 receptor tyrosine kinase 1 antibody
    • ERBB antibody
    • ERBB1 antibody
    • Errp antibody
    • HER1 antibody
    • mENA antibody
    • NISBD2 antibody
    • Oncogen ERBB antibody
    • PIG61 antibody
    • Proto-oncogene c-ErbB-1 antibody
    • Receptor tyrosine protein kinase ErbB 1 antibody
    • Receptor tyrosine-protein kinase ErbB-1 antibody
    • SA7 antibody
    • Species antigen 7 antibody
    • Urogastrone antibody
    • v-erb-b Avian erythroblastic leukemia viral oncogen homolog antibody
    • wa2 antibody
    • Wa5 antibody
    see all

Anti-EGFR antibody images

  • Lane 1 : Anti-EGFR antibody (ab47439) at 1/500 dilution
    Lane 2 : Anti-EGFR antibody (ab47439) at 1/500 dilution (Pre-incubated with blocking peptide.)

    Lane 1 : Extracts from A431 cells treated with EGF (40µM, 10min).
    Lane 2 : Extracts from A431 cells treated with EGF (40µM, 10min).


    Predicted band size : 134 kDa
    Observed band size : 170 kDa (why is the actual band size different from the predicted?)
  • Ab47439, at dilution of 1/50, staining EFGR in paraffin embedded human breast carcinoma tissue by Immunohistochemistry.
    Right image: pre-incubation with blocking peptide was performed.

References for Anti-EGFR antibody (ab47439)

ab47439 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting Abcam.   I have confirmed with the laboratory that we have not tested the antibody for cross reactivity with erbb2 or 3. Based on the sequence homology, the antibody only can react with EGFR.   I hope this information i...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"