Overview

  • Product nameAnti-EGFR antibody
    See all EGFR primary antibodies
  • Description
    Rabbit polyclonal to EGFR
  • SpecificityAb47479 detects endogenous levels of total EGFR protein.
  • Tested applicationsELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    The antiserum was produced against synthesized non phosphopeptide derived from human EGFR around the phosphorylation site of tyrosine 1197 (A-E-YP-L-R).

  • Positive control
    • Extracts from A431 cells.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab47479 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/20000.
WB 1/500 - 1/1000. Detects a band of approximately 175 kDa (predicted molecular weight: 134 kDa).
  • Application notesIs unsuitable for IHC-P.
  • Target

    • Information by UniProt
    • Database links
    • Alternative names
      • Avian erythroblastic leukemia viral (v erb b) oncogene homolog antibody
      • Cell growth inhibiting protein 40 antibody
      • Cell proliferation inducing protein 61 antibody
      • EGF R antibody
      • EGFR antibody
      • EGFR_HUMAN antibody
      • Epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog) antibody
      • Epidermal growth factor receptor (erythroblastic leukemia viral (v erb b) oncogene homolog avian) antibody
      • Epidermal growth factor receptor antibody
      • erb-b2 receptor tyrosine kinase 1 antibody
      • ERBB antibody
      • ERBB1 antibody
      • Errp antibody
      • HER1 antibody
      • mENA antibody
      • NISBD2 antibody
      • Oncogen ERBB antibody
      • PIG61 antibody
      • Proto-oncogene c-ErbB-1 antibody
      • Receptor tyrosine protein kinase ErbB 1 antibody
      • Receptor tyrosine-protein kinase ErbB-1 antibody
      • SA7 antibody
      • Species antigen 7 antibody
      • Urogastrone antibody
      • v-erb-b Avian erythroblastic leukemia viral oncogen homolog antibody
      • wa2 antibody
      • Wa5 antibody
      see all

    Anti-EGFR antibody images

    • All lanes : Anti-EGFR antibody (ab47479) at 1/500 dilution

      Lane 1 : Extracts from A431 cells treated with EGF (40µM, 10min). No peptide.
      Lane 2 : Extracts from A431 cells treated with EGF (40µM, 10min). Synthetic peptide present.


      Predicted band size : 134 kDa
      Observed band size : 175 kDa (why is the actual band size different from the predicted?)

    References for Anti-EGFR antibody (ab47479)

    ab47479 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Mouse Tissue sections (Skin)
    Specification Skin
    Fixative Formaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate
    Permeabilization No
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
    Username

    Abcam user community

    Verified customer

    Submitted Apr 02 2008

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"