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Anti-EGFR antibody (ab51128)

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Overview

Product name

Anti-EGFR antibody
See all EGFR products (87) ...

Description

Rabbit polyclonal to EGFR

Specificity

ab51128 detects endogenous levels of total EGFR protein.

Tested applications

IHC-P, ELISAmore details

Cross reactivity

Reacts with

Mouse, Rat, Human

Immunogen

Synthesized non-phosphopeptide derived from human EGFR around the phosphorylation site of serine 1070-1071 (R-Y-SP-SP-D).

Positive control

Human breast carcinoma tissue.

Properties

Form

Liquid

Storage instructions

Store at -20°C. Stable for 12 months at -20°C

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Clonality

Polyclonal

Isotype

IgG

Research areas

Cancer >> Tumor biomarkers >> Receptors
Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Growth factor receptors
Cancer >> Signal transduction >> Protein phosphorylation >> Tyrosine kinases >> Receptor tyrosine kinases
Signal Transduction >> Growth Factors/Hormones >> EGF
Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Receptor Tyrosine Kinases

  • Immunohistochemistry (Paraffin-embedded sections) - EGFR antibody (ab51128)Immunohistochemistry (Paraffin-embedded sections) - EGFR antibody (ab51128) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab51128 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 1 Image

    IHC-P

     IHC-P: 1/50 - 1/100.

    IHC-P: 1/50 - 1/100.

  • ELISA

     ELISA: 1/20000

    ELISA: 1/20000

Target

Function

Receptor for EGF, but also for other members of the EGF family, as TGF-alpha, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. Is involved in the control of cell growth and differentiation. Phosphorylates MUC1 in breast cancer cells and increases the interaction of MUC1 with SRC and CTNNB1/beta-catenin.
Isoform 2 may act as an antagonist of EGF action.

Tissue specificity

Ubiquitously expressed. Isoform 2 is also expressed in ovarian cancers.

Involvement in disease

Defects in EGFR are associated with lung cancer (LNCR) [MIM:211980].

Sequence similarities

Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
Contains 1 protein kinase domain.

Post-translational
modifications

Phosphorylation of Ser-695 is partial and occurs only if Thr-693 is phosphorylated.
Monoubiquitinated and polyubiquitinated upon EGF stimulation; which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting. Polyubiquitin linkage is mainly through 'Lys-63', but linkage through 'Lys-48', 'Lys-11' and 'Lys-29' also occur.

Cellular localization

Secreted and Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus membrane. Nucleus membrane. Endosome. In response to EGF, translocated from the cell membrane to the nucleus via Golgi and ER. Co-localizes with TNK2 on the endosomes.

Target information above from: UniProt accessionP00533 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • EGFR antibody
  • Avian erythroblastic leukemia viral (v erb b) oncogene homolog antibody
  • Cell growth inhibiting protein 40 antibody
  • Cell proliferation inducing protein 61 antibody
  • EGF R antibody
  • EGFR antibody
  • EGFR_HUMAN antibody
  • Epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog) antibody
  • Epidermal growth factor receptor (erythroblastic leukemia viral (v erb b) oncogene homolog avian) antibody
  • Epidermal growth factor receptor antibody
  • ERBB antibody
  • ERBB1 antibody
  • HER1 antibody
  • mENA antibody
  • PIG61 antibody
  • Proto-oncogene c-ErbB-1 antibody
  • Receptor tyrosine protein kinase ErbB 1 antibody
  • Receptor tyrosine-protein kinase ErbB-1 antibody
  • wa2 antibody
  • Wa5 antibody
see all

Database links

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Anti-EGFR antibody images:

  Immunohistochemistry (Paraffin-embedded sections) - EGFR antibody (ab51128)

Immunohistochemistry (Paraffin-embedded sections) - EGFR antibody (ab51128)

This image shows paraffin-embedded human breast carcinoma tissue stained with ab51128 at a dilution of 1/50 - 1/100. Right hand image: sample treated with immunising peptide; left hand image: untreated sample.

References for Anti-EGFR antibody (ab51128)

ab51128 has not yet been referenced specifically in any publications.

Publishing research using ab51128? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"