EGFR (pY1045) + total EGFR Human ELISA Kit (ab126437)
- Product nameEGFR (pY1045) + total EGFR Human ELISA Kit
- Tests1 x 96 well plate
- Sample typeCell Lysate
- Assay typeSandwich
- Assay time5h 00m
- Species reactivityReacts with: Human
- Product overview
ab126437 is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in cell lysates. By determining phosphorylated EGFR protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.
This sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human phospho-EGFR (Tyr1045) and total EGFR (help normalize the results of phospho-EGFR from different cell lysate being compared). An anti-EGFR antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and phosphorylated and total EGFR present in a sample is bound to the wells by the immobilized antibody. The wells are washed and anti-EGFR (Tyr1045) or anti-total-EGFR antibody is used to detect phosphorylated or non-phosphorylated EGFR. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG or HRP-Streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of EGFR (Tyr1045) or total EGFR bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
- Tested applicationsSandwich ELISA more details
- Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 1 x 96 tests 20X Wash Buffer Concentrate Item B 1 x 25ml 2X Cell Lysate Buffer Item J 1 x 5ml 600X HRP-Streptavidin Concentrate Item G 1 x 200µl Anti-phospho-EGFR (Tyr 1045) 1 x 11µl Assay Diluent: 5x concentrated buffer 1 x 15ml Biotinylated Anti-EGFR: goat anti-human EGFR 1 vial EGFR Microplate (12 strips x 8 wells) coated with monoclonal anti-EGFR antibody 1 unit HRP-conjugated anti-rabbit IgG: 1000x HRP-conjugated anti-rabbit IgG concentrate 1 x 25µl Positive Control: lyophilized powder from A431 cell lysate 1 vial Stop Solution Item I 1 x 8ml TMB One-Step Substrate Reagent Item H 1 x 12ml
Isoform 2 may act as an antagonist of EGF action.
Contains 1 protein kinase domain.
modificationsPhosphorylation of Ser-695 is partial and occurs only if Thr-693 is phosphorylated.
Monoubiquitinated and polyubiquitinated upon EGF stimulation; which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting. Polyubiquitin linkage is mainly through 'Lys-63', but linkage through 'Lys-48', 'Lys-11' and 'Lys-29' also occur.
- Avian erythroblastic leukemia viral (v erb b) oncogene homologCell growth inhibiting protein 40Cell proliferation inducing protein 61
- EGFREGFR_HUMANEpidermal growth factor receptorERBBERBB1HER1mENAPIG61Proto-oncogene c-ErbB-1Receptor tyrosine-protein kinase ErbB-1
Our Abpromise guarantee covers the use of ab126437 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
EGFR (pY1045) + total EGFR Human ELISA Kit images
A431 cells were treated with recombinant Human EGF at 37°C for 20 min. Solubilize cells at 2 x 107 cells/ml in Cell Lysate Buffer. Serial dilutions of lysates were analyzed in this ELISA.
A431 cells were treated or untreated with 100 ng/ml recombinant Human EGF for 10 min. Cell lysates were analyzed using this phosphoELISA.
A431 cells were treated or untreated with 100 ng/ml recombinant human EGF for 10 min. Cell lysates were analyzed by Western Blot.
References for EGFR (pY1045) + total EGFR Human ELISA Kit (ab126437)
ab126437 has not yet been referenced specifically in any publications.