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Overview

  • Product nameEGFR (pY1045) + total EGFR Human ELISA Kit
  • Tests
    1 x 96 well plate
  • Sample type
    Cell Lysate
  • Assay typeSandwich
  • Assay time
    5h 00m
  • Species reactivity
    Reacts with: Human
  • Product overview

    ab126437 is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in cell lysates. By determining phosphorylated EGFR protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.

    This sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human phospho-EGFR (Tyr1045) and total EGFR (help normalize the results of phospho-EGFR from different cell lysate being compared). An anti-EGFR antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and phosphorylated and total EGFR present in a sample is bound to the wells by the immobilized antibody. The wells are washed and anti-EGFR (Tyr1045) or anti-total-EGFR antibody is used to detect phosphorylated or non-phosphorylated EGFR. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG or HRP-Streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of EGFR (Tyr1045) or total EGFR bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applicationsSandwich ELISA more details

Properties

  • FunctionReceptor for EGF, but also for other members of the EGF family, as TGF-alpha, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. Is involved in the control of cell growth and differentiation. Phosphorylates MUC1 in breast cancer cells and increases the interaction of MUC1 with SRC and CTNNB1/beta-catenin.
    Isoform 2 may act as an antagonist of EGF action.
  • Tissue specificityUbiquitously expressed. Isoform 2 is also expressed in ovarian cancers.
  • Involvement in diseaseDefects in EGFR are associated with lung cancer (LNCR) [MIM:211980].
  • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications    Phosphorylation of Ser-695 is partial and occurs only if Thr-693 is phosphorylated.
    Monoubiquitinated and polyubiquitinated upon EGF stimulation; which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting. Polyubiquitin linkage is mainly through 'Lys-63', but linkage through 'Lys-48', 'Lys-11' and 'Lys-29' also occur.
  • Cellular localizationSecreted and Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus membrane. Nucleus membrane. Endosome. In response to EGF, translocated from the cell membrane to the nucleus via Golgi and ER. Co-localizes with TNK2 on the endosomes.

    • Target information above from: UniProt accession P00533 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
    • Alternative names
        Avian erythroblastic leukemia viral (v erb b) oncogene homologCell growth inhibiting protein 40Cell proliferation inducing protein 61
        EGFREGFR_HUMANEpidermal growth factor receptorERBBERBB1HER1mENAPIG61Proto-oncogene c-ErbB-1Receptor tyrosine-protein kinase ErbB-1
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab126437 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      Sandwich ELISA sELISA

      EGFR (pY1045) + total EGFR Human ELISA Kit images

      • Sandwich ELISA - EGFR (pY1045) + total EGFR Human ELISA Kit (ab126437)
        Sandwich ELISA - EGFR (pY1045) + total EGFR Human ELISA Kit (ab126437)
        A431 cells were treated with recombinant Human EGF at 37°C for 20 min. Solubilize cells at 2 x 107 cells/ml in Cell Lysate Buffer. Serial dilutions of lysates were analyzed in this ELISA.
      • Sandwich ELISA - EGFR (pY1045) + total EGFR Human ELISA Kit (ab126437)
        Sandwich ELISA - EGFR (pY1045) + total EGFR Human ELISA Kit (ab126437)
        A431 cells were treated or untreated with 100 ng/ml recombinant Human EGF for 10 min. Cell lysates were analyzed using this phosphoELISA.
      • Western blot - EGFR (pY1045) + total EGFR Human ELISA Kit (ab126437)
        Western blot - EGFR (pY1045) + total EGFR Human ELISA Kit (ab126437)
        A431 cells were treated or untreated with 100 ng/ml recombinant human EGF for 10 min. Cell lysates were analyzed by Western Blot.

      Protocols

      References for EGFR (pY1045) + total EGFR Human ELISA Kit (ab126437)

      ab126437 has not yet been referenced specifically in any publications.

      Publishing research using ab126437 ? Please let us know so that we can cite the reference in this datasheet

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