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Products:Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Receptor Tyrosine Kinases
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If your product does not perform as described on this datasheet, we will refund or replace your product...
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
![Western blot - EGFR (phospho S1047) antibody [1H9] (ab24918)](/ps/datasheet/Images/24/ab24918/ab24918_1.jpg)
Western blot - EGFR (phospho S1047) antibody [1H9] (ab24918)
All lanes : Anti-EGFR (phospho S1047) antibody [1H9] (ab24918) at 0.5 µg/ml (Serum starved HepG2 cells were treated for 15 minutes as indicated. Whole cell lysates were separated by SDS-PAGE. )
Lane 1 : Control
Lane 2 : PMA
Lane 3 : Forskolin
Lane 4 : LPA
Lane 5 : Sorbit
Lane 6 : Anisomycin
Lane 7 : Lonomycin
Lane 8 : Taxol
Lysates/proteins at 20000 cells per lane.
Secondary
A HRP-conjugated antibody
developed using the ECL technique
Predicted band size : 134 kDa
Exposure time : 30 seconds
Recommended blocking buffer CPPT: 0.5% (w/v) casein, 1% (w/v) PEG 4000, 1% (w/v) Polyvinylpyrrolidone (PVP), 0.1% Tween 20, 10mM Tris/HCl, pH 7.4, 150mM Sodium Chloride.
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