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Western blot - EGFR (phospho S1047) antibody [1H9] (ab24918)
Western blot - EGFR (phospho S1047) antibody [1H9] (ab24918)
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Product Name 

EGFR (phospho S1047) antibody [1H9]

 

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Product type 

Primary antibodies

Description 

Mouse monoclonal [1H9] to EGFR (phospho S1047)

Immunogen 

Synthetic phosphopeptide (Human) corresponding to amino acid residues surrounding Serine 1047.

Reacts with 
(species key)

Hu, Ms, Dog

Specificity 

This antibody specifically recognizes EGFR phosphorylated at Serine 1047.

Tested applications 
(see key)

ELISA, ICC, WB


Abreviews 

Customer reviews
(see key)


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Application notes 
(see key)

Recommended dilutions
ELISA: Use at a concentration of 0.1 µg/ml. ICC: Use at a concentration of 0.1-1.0 µg/ml. WB: Use at a concentration of 0.5 µg/ml. Predicted molecular weight: 134 kDa. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

The following positive control is supplied with with this antibody:-

Cell lysate of 1 x 106 vanadate treated HepG2 cells.
Format: Cell lysate, liquid that has been reconstituted with 100µl water and 100ul of SDS-PAGE sample buffer.

Cellular localization 

Type I membrane protein (isoform 1) and secreted (isoform 2).

Research areas 

Cancer >> Tumor biomarkers >> Receptors
Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Growth factor receptors
Cancer >> Signal transduction >> Protein phosphorylation >> Tyrosine kinases >> Receptor tyrosine kinases
Signal Transduction >> Growth Factors/Hormones >> EGF
Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Receptor Tyrosine Kinases

Relevance 

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. The protein kinase family is one of the largest families of proteins in eukaryotes, classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. Epidermal Growth factor receptor (EGFR) is the prototype member of the type 1 receptor tyrosine kinases. EGFR overexpression in tumors indicates poor prognosis and is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, stomach and in squamous cell carcinoma.

 

EGFR (phospho S1047) antibody [1H9] (ab24918)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

404306    (Dog)
1956    (Human)
13649    (Mouse)

Omim

    

131550    (Human)

SwissProt

    

P00533    (Human)
Q01279    (Mouse)

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

1H9

Isotype 

IgG1

Storage buffer 

Preservative: 0.09% Sodium Azide
Constituents: 2X PBS, Polyethylene glycol, Sucrose

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Purification notes 

Thiophilic adsorption and size exclusion chromatography.

Form 

Liquid

Concentration 

0.100 mg/ml

Storage instructions 

Store at -20°C. Stable for 12 months at -20°C

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this EGFR (phospho S1047) antibody [1H9] is on this datasheet. But please do contact us if you would like any reassurance!


See below for EGFR (phospho S1047) antibody [1H9] images, references, products related to ab24918 and other tools.

 

EGFR (phospho S1047) antibody [1H9] images:

Product image  Western blot - EGFR (phospho S1047) antibody [1H9] (ab24918)

Western blot - EGFR (phospho S1047) antibody [1H9] (ab24918)

All lanes : EGFR (phospho S1047) antibody [1H9] (ab24918) at 0.5 µg/ml (Serum starved HepG2 cells were treated for 15 minutes as indicated. Whole cell lysates were separated by SDS-PAGE. )

Lane 1 : Control
Lane 2 : PMA
Lane 3 : Forskolin
Lane 4 : LPA
Lane 5 : Sorbit
Lane 6 : Anisomycin
Lane 7 : Lonomycin
Lane 8 : Taxol

Lysates/proteins at 20000 cells per lane.

Secondary
A HRP-conjugated antibody
developed using the ECL technique

Predicted band size : 134 kDa


Exposure time : 30 seconds

Recommended blocking buffer CPPT: 0.5% (w/v) casein, 1% (w/v) PEG 4000, 1% (w/v) Polyvinylpyrrolidone (PVP), 0.1% Tween 20, 10mM Tris/HCl, pH 7.4, 150mM Sodium Chloride.

 
 

Search PubMed (MEDLINE) for references to EGFR

 

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EGFR (phospho S1047) antibody [1H9] - related products:

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Loading controls

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