Anti-EGFR (phospho S1047) antibody [1H9] (ab24918)

Overview

  • Product nameAnti-EGFR (phospho S1047) antibody [1H9]
    See all EGFR primary antibodies
  • Description
    Mouse monoclonal [1H9] to EGFR (phospho S1047)
  • Tested applicationsWB, ELISA, ICCmore details
  • Species reactivity
    Reacts with: Mouse, Dog, Human
  • Immunogen

    Synthetic phosphopeptide (Human) corresponding to amino acid residues surrounding Serine 1047.

  • Positive control
    • The following positive control is supplied with with this antibody:- Cell lysate of 1 x 106 vanadate treated HepG2 cells. Format: Cell lysate, liquid that has been reconstituted with 100µl water and 100ul of SDS-PAGE sample buffer.

Properties

Applications

Our Abpromise guarantee covers the use of ab24918 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
ICC
  • Application notesELISA: Use at a concentration of 0.1 µg/ml. ICC: Use at a concentration of 0.1-1.0 µg/ml. WB: Use at a concentration of 0.5 µg/ml. Predicted molecular weight: 134 kDa. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Information by UniProt
    • Database links
    • Alternative names
      • Avian erythroblastic leukemia viral (v erb b) oncogene homolog antibody
      • Cell growth inhibiting protein 40 antibody
      • Cell proliferation inducing protein 61 antibody
      • EGF R antibody
      • EGFR antibody
      • EGFR_HUMAN antibody
      • Epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog) antibody
      • Epidermal growth factor receptor (erythroblastic leukemia viral (v erb b) oncogene homolog avian) antibody
      • Epidermal growth factor receptor antibody
      • erb-b2 receptor tyrosine kinase 1 antibody
      • ERBB antibody
      • ERBB1 antibody
      • Errp antibody
      • HER1 antibody
      • mENA antibody
      • NISBD2 antibody
      • Oncogen ERBB antibody
      • PIG61 antibody
      • Proto-oncogene c-ErbB-1 antibody
      • Receptor tyrosine protein kinase ErbB 1 antibody
      • Receptor tyrosine-protein kinase ErbB-1 antibody
      • SA7 antibody
      • Species antigen 7 antibody
      • Urogastrone antibody
      • v-erb-b Avian erythroblastic leukemia viral oncogen homolog antibody
      • wa2 antibody
      • Wa5 antibody
      see all

    Anti-EGFR (phospho S1047) antibody [1H9] images

    • All lanes : Anti-EGFR (phospho S1047) antibody [1H9] (ab24918) at 0.5 µg/ml (Serum starved HepG2 cells were treated for 15 minutes as indicated. Whole cell lysates were separated by SDS-PAGE. )

      Lane 1 : Control
      Lane 2 : PMA
      Lane 3 : Forskolin
      Lane 4 : LPA
      Lane 5 : Sorbit
      Lane 6 : Anisomycin
      Lane 7 : Lonomycin
      Lane 8 : Taxol

      Lysates/proteins at 20000 cells per lane.

      Secondary
      A HRP-conjugated antibody
      developed using the ECL technique

      Predicted band size : 134 kDa


      Exposure time : 30 seconds

    References for Anti-EGFR (phospho S1047) antibody [1H9] (ab24918)

    ab24918 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"