EGFR (phospho S1047) antibody [1H9] (ab24918)

Product Name 

EGFR (phospho S1047) antibody [1H9]

Product type 

Primary antibodies

Description 

Mouse monoclonal [1H9] to EGFR (phospho S1047)

Immunogen 

Synthetic phosphopeptide (Human) corresponding to amino acid residues surrounding Serine 1047.

Reacts with 
(species key)

Hu, Ms, Dog

Specificity 

This antibody specifically recognizes EGFR phosphorylated at Serine 1047.

Tested applications 
(see key)

ELISA, ICC, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
ELISA: Use at a concentration of 0.1 µg/ml. ICC: Use at a concentration of 0.1-1.0 µg/ml. WB: Use at a concentration of 0.5 µg/ml. Predicted molecular weight: 134 kDa. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

The following positive control is supplied with with this antibody:-

Cell lysate of 1 x 10⁶ vanadate treated HepG2 cells.
Format: Cell lysate, liquid that has been reconstituted with 100µl water and 100ul of SDS-PAGE sample buffer.

Cellular localization 

Type I membrane protein (isoform 1) and secreted (isoform 2).

Research areas 

Cancer >> Tumor biomarkers >> Receptors
Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Growth factor receptors
Cancer >> Signal transduction >> Protein phosphorylation >> Tyrosine kinases >> Receptor tyrosine kinases
Signal Transduction >> Growth Factors/Hormones >> EGF
Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Receptor Tyrosine Kinases

Relevance 

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. The protein kinase family is one of the largest families of proteins in eukaryotes, classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. Epidermal Growth factor receptor (EGFR) is the prototype member of the type 1 receptor tyrosine kinases. EGFR overexpression in tumors indicates poor prognosis and is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, stomach and in squamous cell carcinoma.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

1H9

Isotype 

IgG1

Storage buffer 

Preservative: 0.09% Sodium Azide
Constituents: 2X PBS, Polyethylene glycol, Sucrose

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Purification notes 

Thiophilic adsorption and size exclusion chromatography.

Form 

Liquid

Concentration 

0.100 mg/ml

Storage instructions 

Store at -20°C. Stable for 12 months at -20°C

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