![Western blot - EGFR (phospho S1047) antibody [1H9] (ab24918) image](#ab24918_1.jpg)
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Products:Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Receptor Tyrosine Kinases
Overview
Product name
Anti-EGFR (phospho S1047) antibody [1H9]
See all EGFR products (87) ...
Description
Mouse monoclonal [1H9] to EGFR (phospho S1047)
Specificity
This antibody specifically recognizes EGFR phosphorylated at Serine 1047.
Tested applications
WB, ELISA, ICCmore details
Cross reactivity
Reacts with
Mouse, Dog, Human
Immunogen
Synthetic phosphopeptide (Human) corresponding to amino acid residues surrounding Serine 1047.
Positive control
The following positive control is supplied with with this antibody:- Cell lysate of 1 x 106 vanadate treated HepG2 cells. Format: Cell lysate, liquid that has been reconstituted with 100µl water and 100ul of SDS-PAGE sample buffer.
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: 2X PBS, Polyethylene glycol, Sucrose
Concentration
Concentration information loading...
Purification notes
Thiophilic adsorption and size exclusion chromatography.
Clonality
Monoclonal
Clone number
1H9
Isotype
IgG1
Research areas
Cancer >> Tumor biomarkers >> Receptors
Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Growth factor receptors
Cancer >> Signal transduction >> Protein phosphorylation >> Tyrosine kinases >> Receptor tyrosine kinases
Signal Transduction >> Growth Factors/Hormones >> EGF
Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Receptor Tyrosine Kinases
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Western blot - EGFR (phospho S1047) antibody [1H9] (ab24918)
![Western blot - EGFR (phospho S1047) antibody [1H9] (ab24918) image](/ps/datasheet/Images/24/ab24918/ab24918_1.jpg)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab24918 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
ELISA
ICC
Application notes
ELISA: Use at a concentration of 0.1 µg/ml. ICC: Use at a concentration of 0.1-1.0 µg/ml. WB: Use at a concentration of 0.5 µg/ml. Predicted molecular weight: 134 kDa. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Receptor for EGF, but also for other members of the EGF family, as TGF-alpha, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. Is involved in the control of cell growth and differentiation. Phosphorylates MUC1 in breast cancer cells and increases the interaction of MUC1 with SRC and CTNNB1/beta-catenin.
Isoform 2 may act as an antagonist of EGF action.
Tissue specificity
Ubiquitously expressed. Isoform 2 is also expressed in ovarian cancers.
Involvement in disease
Defects in EGFR are associated with lung cancer (LNCR) [MIM:211980].
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
Contains 1 protein kinase domain.
Post-translational
modifications
Phosphorylation of Ser-695 is partial and occurs only if Thr-693 is phosphorylated.
Monoubiquitinated and polyubiquitinated upon EGF stimulation; which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting. Polyubiquitin linkage is mainly through 'Lys-63', but linkage through 'Lys-48', 'Lys-11' and 'Lys-29' also occur.
Cellular localization
Secreted and Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus membrane. Nucleus membrane. Endosome. In response to EGF, translocated from the cell membrane to the nucleus via Golgi and ER. Co-localizes with TNK2 on the endosomes.
Target information above from: UniProt accessionP00533
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- EGFR antibody
- Avian erythroblastic leukemia viral (v erb b) oncogene homolog antibody
- Avian erythroblastic leukemia viral (verbb) oncogene homolog antibody
see all
Database links
- Entrez Gene: 404306 Dog
- Entrez Gene: 1956 Human
- Entrez Gene: 13649 Mouse
- Omim: 131550 Human
- SwissProt: P00533 Human
- SwissProt: Q01279 Mouse
- Unigene: 488293 Human
- Unigene: 420648 Mouse
see all
Anti-EGFR (phospho S1047) antibody [1H9] images:
Western blot - EGFR (phospho S1047) antibody [1H9] (ab24918)
All lanes : Anti-EGFR (phospho S1047) antibody [1H9] (ab24918) at 0.5 µg/ml (Serum starved HepG2 cells were treated for 15 minutes as indicated. Whole cell lysates were separated by SDS-PAGE. )
Lane 1 : Control
Lane 2 : PMA
Lane 3 : Forskolin
Lane 4 : LPA
Lane 5 : Sorbit
Lane 6 : Anisomycin
Lane 7 : Lonomycin
Lane 8 : Taxol
Lysates/proteins at 20000 cells per lane.
Secondary
A HRP-conjugated antibody
developed using the ECL technique
Predicted band size : 134 kDa
Exposure time : 30 seconds
Recommended blocking buffer CPPT: 0.5% (w/v) casein, 1% (w/v) PEG 4000, 1% (w/v) Polyvinylpyrrolidone (PVP), 0.1% Tween 20, 10mM Tris/HCl, pH 7.4, 150mM Sodium Chloride.
References for Anti-EGFR (phospho S1047) antibody [1H9] (ab24918)
ab24918 has not yet been referenced specifically in any publications.
Publishing research using ab24918? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"