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Anti-EGFR (phospho S1047) antibody [1H9] (ab24918)

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Overview

  • Product nameAnti-EGFR (phospho S1047) antibody [1H9]See all EGFR primary antibodies ...
  • Description
    Mouse monoclonal [1H9] to EGFR (phospho S1047)
  • SpecificityThis antibody specifically recognizes EGFR phosphorylated at Serine 1047.
  • Tested applicationsWB, ELISA, ICC more details
  • Species reactivity
    Reacts with: Mouse, Dog, Human
  • Immunogen

    Synthetic phosphopeptide (Human) corresponding to amino acid residues surrounding Serine 1047.

  • Positive controlThe following positive control is supplied with with this antibody:- Cell lysate of 1 x 106 vanadate treated HepG2 cells. Format: Cell lysate, liquid that has been reconstituted with 100µl water and 100ul of SDS-PAGE sample buffer.

Properties

Applications

Our Abpromise guarantee covers the use of ab24918 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB
ELISA
ICC
  • Application notesELISA: Use at a concentration of 0.1 µg/ml. ICC: Use at a concentration of 0.1-1.0 µg/ml. WB: Use at a concentration of 0.5 µg/ml. Predicted molecular weight: 134 kDa. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • FunctionReceptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin.
      Isoform 2 may act as an antagonist of EGF action.
    • Tissue specificityUbiquitously expressed. Isoform 2 is also expressed in ovarian cancers.
    • Involvement in diseaseDefects in EGFR are associated with lung cancer (LNCR) [MIM:211980]. LNCR is a common malignancy affecting tissues of the lung. The most common form of lung cancer is non-small cell lung cancer (NSCLC) that can be divided into 3 major histologic subtypes: squamous cell carcinoma, adenocarcinoma, and large cell lung cancer. NSCLC is often diagnosed at an advanced stage and has a poor prognosis.
    • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications      Phosphorylation at Ser-695 is partial and occurs only if Thr-693 is phosphorylated. Phosphorylation at Thr-678 and Thr-693 by PRKD1 inhibits EGF-induced MAPK8/JNK1 activation. Dephosphorylation by PTPRJ prevents endocytosis and stabilizes the receptor at the plasma membrane. Autophosphorylation at Tyr-1197 is stimulated by methylation at Arg-1199 and enhances interaction with PTPN6. Autophosphorylation at Tyr-1092 and/or Tyr-1110 recruits STAT3.
      Monoubiquitinated and polyubiquitinated upon EGF stimulation; which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting. Polyubiquitin linkage is mainly through 'Lys-63', but linkage through 'Lys-48', 'Lys-11' and 'Lys-29' also occur. Deubiquitinated by OTUD7B, preventing degradation.
      Methylated. Methylation at Arg-1199 by PRMT5 positively stimulates phosphorylation at Tyr-1197.
    • Cellular localizationSecreted and Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus membrane. Nucleus membrane. Endosome. Endosome membrane. In response to EGF, translocated from the cell membrane to the nucleus via Golgi and ER. Endocytosed upon activation by ligand.

      • Target information above from: UniProt accession P00533 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
      see all
    • Alternative names
        Avian erythroblastic leukemia viral (v erb b) oncogene homolog antibodyAvian erythroblastic leukemia viral (verbb) oncogene homolog antibodyCell growth inhibiting protein 40 antibody
        Cell proliferation inducing protein 61 antibodyEGF R antibodyEGFR antibodyEGFR antibodyEGFR_HUMAN antibodyEpidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog) antibodyEpidermal growth factor receptor (erythroblastic leukemia viral (v erb b) oncogene homolog avian) antibodyEpidermal growth factor receptor antibodyerbb 1 antibodyErbb antibodyErbb1 antibodyERBB1 antibodyErrp antibodyHER1 antibodymENA antibodyOncogene ERBB antibodyPIG61 antibodyProto-oncogene c-ErbB-1 antibodyReceptor tyrosine protein kinase ErbB 1 antibodyReceptor tyrosine protein kinase ErbB1 antibodyReceptor tyrosine-protein kinase ErbB-1 antibodyUrogastrone antibodywa2 antibodyWa5 antibody
      see all

    Anti-EGFR (phospho S1047) antibody [1H9] images

    • Western blot - EGFR (phospho S1047) antibody [1H9] (ab24918)
      Western blot - EGFR (phospho S1047) antibody [1H9] (ab24918)
      All lanes : Anti-EGFR (phospho S1047) antibody [1H9] (ab24918) at 0.5 µg/ml (Serum starved HepG2 cells were treated for 15 minutes as indicated. Whole cell lysates were separated by SDS-PAGE. )

      Lane 1 : Control
      Lane 2 : PMA
      Lane 3 : Forskolin
      Lane 4 : LPA
      Lane 5 : Sorbit
      Lane 6 : Anisomycin
      Lane 7 : Lonomycin
      Lane 8 : Taxol

      Lysates/proteins at 20000 cells per lane.

      Secondary
      A HRP-conjugated antibody
      developed using the ECL technique

      Predicted band size : 134 kDa


      Exposure time : 30 seconds

    References for Anti-EGFR (phospho S1047) antibody [1H9] (ab24918)

    ab24918 has not yet been referenced specifically in any publications.

    Publishing research using ab24918 ? Please let us know so that we can cite the reference in this datasheet

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