![Western blot - EGFR (phospho S1070 + S1071) antibody [EP2259IHCY] (ab76300) image](#egfr1.gif)
![Immunocytochemistry/ Immunofluorescence - Anti-EGFR (phospho S1070 + S1071) antibody [EP2259IHCY] (ab76300) image](#egfr2.jpg)
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Products:Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Receptor Tyrosine Kinases
Overview
Product name
Anti-EGFR (phospho S1070 + S1071) antibody [EP2259IHCY]
See all EGFR products (87) ...
Description
Rabbit monoclonal [EP2259IHCY] to EGFR (phospho S1070 + S1071)
Tested applications
WB, IP, ICC/IFmore details
Cross reactivity
Reacts with
Human
Does not react with
Mouse, Rat
Immunogen
A synthetic phospho-peptide corresponding to residues surrounding Serine 1070 and 1071 of human EGF receptor.
Positive control
A431 cells adn A431 cell lysates
General notes
Produced under U.S. Patent No. 5,675,063.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, Tissue culture supernatant, 0.15M Sodium chloride, 50mM Tris glycine, pH 7.4
Purity
Tissue culture supernatant
Clonality
Monoclonal
Clone number
EP2259IHCY
Isotype
IgG
Research areas
Cancer >> Tumor biomarkers >> Receptors
Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Growth factor receptors
Cancer >> Signal transduction >> Protein phosphorylation >> Tyrosine kinases >> Receptor tyrosine kinases
Signal Transduction >> Growth Factors/Hormones >> EGF
Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Receptor Tyrosine Kinases
Applications
Show applications key
Our Abpromise guarantee covers the use of ab76300 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500000 - 1/1000000.Detects a band of approximately 150 kDa (predicted molecular weight: 134 kDa).
IP
IP: 1/30IP: 1/30
ICC/IF: 1/100 - 1/250.
Application notes
Is unsuitable for ,Flow Cyt or IHC-P.
Target
Function
Receptor for EGF, but also for other members of the EGF family, as TGF-alpha, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. Is involved in the control of cell growth and differentiation. Phosphorylates MUC1 in breast cancer cells and increases the interaction of MUC1 with SRC and CTNNB1/beta-catenin.
Isoform 2 may act as an antagonist of EGF action.
Tissue specificity
Ubiquitously expressed. Isoform 2 is also expressed in ovarian cancers.
Involvement in disease
Defects in EGFR are associated with lung cancer (LNCR) [MIM:211980].
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
Contains 1 protein kinase domain.
Post-translational
modifications
Phosphorylation of Ser-695 is partial and occurs only if Thr-693 is phosphorylated.
Monoubiquitinated and polyubiquitinated upon EGF stimulation; which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting. Polyubiquitin linkage is mainly through 'Lys-63', but linkage through 'Lys-48', 'Lys-11' and 'Lys-29' also occur.
Cellular localization
Secreted and Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus membrane. Nucleus membrane. Endosome. In response to EGF, translocated from the cell membrane to the nucleus via Golgi and ER. Co-localizes with TNK2 on the endosomes.
Target information above from: UniProt accessionP00533
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- EGFR antibody
- Avian erythroblastic leukemia viral (v erb b) oncogene homolog antibody
- Cell growth inhibiting protein 40 antibody
see all
Database links
- Entrez Gene: 1956 Human
- Omim: 131550 Human
- SwissProt: P00533 Human
- Unigene: 488293 Human
Anti-EGFR (phospho S1070 + S1071) antibody [EP2259IHCY] images:
Western blot - EGFR (phospho S1070 + S1071) antibody [EP2259IHCY] (ab76300)
![Western blot - EGFR (phospho S1070 + S1071) antibody [EP2259IHCY] (ab76300)](/ps/datasheet/Images/76/ab76300/egfr1.gif)
All lanes : Anti-EGFR (phospho S1070 + S1071) antibody [EP2259IHCY] (ab76300) at 1/1000000 dilution
Lane 1 : A431 cell lysate
Lane 2 : A431 cell lysate treated with EGF
Lysates/proteins at 10 µg per lane.
Secondary
HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size : 134 kDa
Observed band size : 180 kDa (why is the actual band size different from the predicted?)
Immunocytochemistry/ Immunofluorescence - Anti-EGFR (phospho S1070 + S1071) antibody [EP2259IHCY] (ab76300)
![Immunocytochemistry/ Immunofluorescence - Anti-EGFR (phospho S1070 + S1071) antibody [EP2259IHCY] (ab76300)](/ps/datasheet/Images/76/ab76300/egfr2.jpg)
Immunofluorescence staining of A431 cells using ab76300 at 1/100-1/250 dilution.
References for Anti-EGFR (phospho S1070 + S1071) antibody [EP2259IHCY] (ab76300)
ab76300 has not yet been referenced specifically in any publications.
Publishing research using ab76300? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"